Transcriptional remodeling during metacyclogenesis in Trypanosoma cruzi I

Metacyclogenesis is one of the most important processes in the life cycle of Trypanosoma cruzi. In this stage, noninfective epimastigotes become infective metacyclic trypomastigotes. However, the transcriptomic changes that occur during this transformation remain uncertain. Illumina RNA-sequencing o...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Lissa Cruz-Saavedra, Gustavo A. Vallejo, Felipe Guhl, Louisa A. Messenger, Juan David Ramírez
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2020
Materias:	trypanosoma cruzi metacyclic trypomastigotes epimastigotes transcriptomic pathways Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/d253bc314b994eea92a8975b7d45e114
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:d253bc314b994eea92a8975b7d45e114
record_format	dspace
spelling	oai:doaj.org-article:d253bc314b994eea92a8975b7d45e1142021-11-17T14:21:58ZTranscriptional remodeling during metacyclogenesis in Trypanosoma cruzi I2150-55942150-560810.1080/21505594.2020.1797274https://doaj.org/article/d253bc314b994eea92a8975b7d45e1142020-12-01T00:00:00Zhttp://dx.doi.org/10.1080/21505594.2020.1797274https://doaj.org/toc/2150-5594https://doaj.org/toc/2150-5608Metacyclogenesis is one of the most important processes in the life cycle of Trypanosoma cruzi. In this stage, noninfective epimastigotes become infective metacyclic trypomastigotes. However, the transcriptomic changes that occur during this transformation remain uncertain. Illumina RNA-sequencing of epimastigotes and metacyclic trypomastigotes belonging to T. cruzi DTU I was undertaken. Sequencing reads were aligned and mapped against the reference genome, differentially expressed genes between the two life cycle stages were identified, and metabolic pathways were reconstructed. Gene expression differed significantly between epimastigotes and metacyclic trypomastigotes. The cellular pathways that were mostly downregulated during metacyclogenesis involved glucose energy metabolism (glycolysis, pyruvate metabolism, the Krebs cycle, and oxidative phosphorylation), amino acid metabolism, and DNA replication. By contrast, the processes where an increase in gene expression was observed included those related to autophagy (particularly Atg7 and Atg8 transcripts), corroborating its importance during metacyclogenesis, endocytosis, by an increase in the expression of the AP-2 complex subunit alpha, protein processing in the endoplasmic reticulum and meiosis. Study findings indicate that in T. cruzi metacyclic trypomastigotes, metabolic processes are decreased, and expression of genes involved in specific cell cycle processes is increased to facilitate transformation to this infective stage.Lissa Cruz-SaavedraGustavo A. VallejoFelipe GuhlLouisa A. MessengerJuan David RamírezTaylor & Francis Grouparticletrypanosoma cruzimetacyclic trypomastigotesepimastigotestranscriptomicpathwaysInfectious and parasitic diseasesRC109-216ENVirulence, Vol 11, Iss 1, Pp 968-979 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	trypanosoma cruzi metacyclic trypomastigotes epimastigotes transcriptomic pathways Infectious and parasitic diseases RC109-216
spellingShingle	trypanosoma cruzi metacyclic trypomastigotes epimastigotes transcriptomic pathways Infectious and parasitic diseases RC109-216 Lissa Cruz-Saavedra Gustavo A. Vallejo Felipe Guhl Louisa A. Messenger Juan David Ramírez Transcriptional remodeling during metacyclogenesis in Trypanosoma cruzi I
description	Metacyclogenesis is one of the most important processes in the life cycle of Trypanosoma cruzi. In this stage, noninfective epimastigotes become infective metacyclic trypomastigotes. However, the transcriptomic changes that occur during this transformation remain uncertain. Illumina RNA-sequencing of epimastigotes and metacyclic trypomastigotes belonging to T. cruzi DTU I was undertaken. Sequencing reads were aligned and mapped against the reference genome, differentially expressed genes between the two life cycle stages were identified, and metabolic pathways were reconstructed. Gene expression differed significantly between epimastigotes and metacyclic trypomastigotes. The cellular pathways that were mostly downregulated during metacyclogenesis involved glucose energy metabolism (glycolysis, pyruvate metabolism, the Krebs cycle, and oxidative phosphorylation), amino acid metabolism, and DNA replication. By contrast, the processes where an increase in gene expression was observed included those related to autophagy (particularly Atg7 and Atg8 transcripts), corroborating its importance during metacyclogenesis, endocytosis, by an increase in the expression of the AP-2 complex subunit alpha, protein processing in the endoplasmic reticulum and meiosis. Study findings indicate that in T. cruzi metacyclic trypomastigotes, metabolic processes are decreased, and expression of genes involved in specific cell cycle processes is increased to facilitate transformation to this infective stage.
format	article
author	Lissa Cruz-Saavedra Gustavo A. Vallejo Felipe Guhl Louisa A. Messenger Juan David Ramírez
author_facet	Lissa Cruz-Saavedra Gustavo A. Vallejo Felipe Guhl Louisa A. Messenger Juan David Ramírez
author_sort	Lissa Cruz-Saavedra
title	Transcriptional remodeling during metacyclogenesis in Trypanosoma cruzi I
title_short	Transcriptional remodeling during metacyclogenesis in Trypanosoma cruzi I
title_full	Transcriptional remodeling during metacyclogenesis in Trypanosoma cruzi I
title_fullStr	Transcriptional remodeling during metacyclogenesis in Trypanosoma cruzi I
title_full_unstemmed	Transcriptional remodeling during metacyclogenesis in Trypanosoma cruzi I
title_sort	transcriptional remodeling during metacyclogenesis in trypanosoma cruzi i
publisher	Taylor & Francis Group
publishDate	2020
url	https://doaj.org/article/d253bc314b994eea92a8975b7d45e114
work_keys_str_mv	AT lissacruzsaavedra transcriptionalremodelingduringmetacyclogenesisintrypanosomacruzii AT gustavoavallejo transcriptionalremodelingduringmetacyclogenesisintrypanosomacruzii AT felipeguhl transcriptionalremodelingduringmetacyclogenesisintrypanosomacruzii AT louisaamessenger transcriptionalremodelingduringmetacyclogenesisintrypanosomacruzii AT juandavidramirez transcriptionalremodelingduringmetacyclogenesisintrypanosomacruzii
_version_	1718425511759183872

Transcriptional remodeling during metacyclogenesis in Trypanosoma cruzi I

Ejemplares similares