Murine cerebral organoids develop network of functional neurons and hippocampal brain region identity

Summary: Brain organoids are in vitro three-dimensional (3D) self-organized neural structures, which can enable disease modeling and drug screening. However, their use for standardized large-scale drug screening studies is limited by their high batch-to-batch variability, long differentiation time (...

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Autores principales:	Francesca Ciarpella, Raluca Georgiana Zamfir, Alessandra Campanelli, Elisa Ren, Giulia Pedrotti, Emanuela Bottani, Andrea Borioli, Davide Caron, Marzia Di Chio, Sissi Dolci, Annika Ahtiainen, Giorgio Malpeli, Giovanni Malerba, Rita Bardoni, Guido Fumagalli, Jari Hyttinen, Francesco Bifari, Gemma Palazzolo, Gabriella Panuccio, Giulia Curia, Ilaria Decimo
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Biological sciences Neuroscience Cell biology Developmental biology Science Q
Acceso en línea:	https://doaj.org/article/d26282c5638e451288e865f40866d9b8
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spelling	oai:doaj.org-article:d26282c5638e451288e865f40866d9b82021-12-02T05:03:30ZMurine cerebral organoids develop network of functional neurons and hippocampal brain region identity2589-004210.1016/j.isci.2021.103438https://doaj.org/article/d26282c5638e451288e865f40866d9b82021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2589004221014097https://doaj.org/toc/2589-0042Summary: Brain organoids are in vitro three-dimensional (3D) self-organized neural structures, which can enable disease modeling and drug screening. However, their use for standardized large-scale drug screening studies is limited by their high batch-to-batch variability, long differentiation time (10–20 weeks), and high production costs. This is particularly relevant when brain organoids are obtained from human induced pluripotent stem cells (iPSCs). Here, we developed, for the first time, a highly standardized, reproducible, and fast (5 weeks) murine brain organoid model starting from embryonic neural stem cells. We obtained brain organoids, which progressively differentiated and self-organized into 3D networks of functional neurons with dorsal forebrain phenotype. Furthermore, by adding the morphogen WNT3a, we generated brain organoids with specific hippocampal region identity. Overall, our results showed the establishment of a fast, robust and reproducible murine 3D in vitro brain model that may represent a useful tool for high-throughput drug screening and disease modeling.Francesca CiarpellaRaluca Georgiana ZamfirAlessandra CampanelliElisa RenGiulia PedrottiEmanuela BottaniAndrea BorioliDavide CaronMarzia Di ChioSissi DolciAnnika AhtiainenGiorgio MalpeliGiovanni MalerbaRita BardoniGuido FumagalliJari HyttinenFrancesco BifariGemma PalazzoloGabriella PanuccioGiulia CuriaIlaria DecimoElsevierarticleBiological sciencesNeuroscienceCell biologyDevelopmental biologyScienceQENiScience, Vol 24, Iss 12, Pp 103438- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biological sciences Neuroscience Cell biology Developmental biology Science Q
spellingShingle	Biological sciences Neuroscience Cell biology Developmental biology Science Q Francesca Ciarpella Raluca Georgiana Zamfir Alessandra Campanelli Elisa Ren Giulia Pedrotti Emanuela Bottani Andrea Borioli Davide Caron Marzia Di Chio Sissi Dolci Annika Ahtiainen Giorgio Malpeli Giovanni Malerba Rita Bardoni Guido Fumagalli Jari Hyttinen Francesco Bifari Gemma Palazzolo Gabriella Panuccio Giulia Curia Ilaria Decimo Murine cerebral organoids develop network of functional neurons and hippocampal brain region identity
description	Summary: Brain organoids are in vitro three-dimensional (3D) self-organized neural structures, which can enable disease modeling and drug screening. However, their use for standardized large-scale drug screening studies is limited by their high batch-to-batch variability, long differentiation time (10–20 weeks), and high production costs. This is particularly relevant when brain organoids are obtained from human induced pluripotent stem cells (iPSCs). Here, we developed, for the first time, a highly standardized, reproducible, and fast (5 weeks) murine brain organoid model starting from embryonic neural stem cells. We obtained brain organoids, which progressively differentiated and self-organized into 3D networks of functional neurons with dorsal forebrain phenotype. Furthermore, by adding the morphogen WNT3a, we generated brain organoids with specific hippocampal region identity. Overall, our results showed the establishment of a fast, robust and reproducible murine 3D in vitro brain model that may represent a useful tool for high-throughput drug screening and disease modeling.
format	article
author	Francesca Ciarpella Raluca Georgiana Zamfir Alessandra Campanelli Elisa Ren Giulia Pedrotti Emanuela Bottani Andrea Borioli Davide Caron Marzia Di Chio Sissi Dolci Annika Ahtiainen Giorgio Malpeli Giovanni Malerba Rita Bardoni Guido Fumagalli Jari Hyttinen Francesco Bifari Gemma Palazzolo Gabriella Panuccio Giulia Curia Ilaria Decimo
author_facet	Francesca Ciarpella Raluca Georgiana Zamfir Alessandra Campanelli Elisa Ren Giulia Pedrotti Emanuela Bottani Andrea Borioli Davide Caron Marzia Di Chio Sissi Dolci Annika Ahtiainen Giorgio Malpeli Giovanni Malerba Rita Bardoni Guido Fumagalli Jari Hyttinen Francesco Bifari Gemma Palazzolo Gabriella Panuccio Giulia Curia Ilaria Decimo
author_sort	Francesca Ciarpella
title	Murine cerebral organoids develop network of functional neurons and hippocampal brain region identity
title_short	Murine cerebral organoids develop network of functional neurons and hippocampal brain region identity
title_full	Murine cerebral organoids develop network of functional neurons and hippocampal brain region identity
title_fullStr	Murine cerebral organoids develop network of functional neurons and hippocampal brain region identity
title_full_unstemmed	Murine cerebral organoids develop network of functional neurons and hippocampal brain region identity
title_sort	murine cerebral organoids develop network of functional neurons and hippocampal brain region identity
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/d26282c5638e451288e865f40866d9b8
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Murine cerebral organoids develop network of functional neurons and hippocampal brain region identity

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