Influence of Citric Acid on the Vitality of Stem Cells from Apical Papilla

The endodontic treatment of immature permanent teeth with necrotic pulp is a serious clinical challenge. The chemical agents, used in regenerative procedures, should be selected not only based on their bactericidal/bacteriostatic properties, but also on their ability to ensure the survival of the pa...

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Autores principales:	Hristov Kr., Gateva N., Stanimirov P., Ishkitiev N., Tsikandelova R., Mihaylova Z.
Formato:	article
Lenguaje:	EN
Publicado:	Sciendo 2018
Materias:	apical papilla stem cells citric acid Medicine R
Acceso en línea:	https://doaj.org/article/d2660059c2ac41a59f6795cf5eb1f9df
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spelling	oai:doaj.org-article:d2660059c2ac41a59f6795cf5eb1f9df2021-12-02T14:59:50ZInfluence of Citric Acid on the Vitality of Stem Cells from Apical Papilla0324-175010.2478/amb-2018-0016https://doaj.org/article/d2660059c2ac41a59f6795cf5eb1f9df2018-12-01T00:00:00Zhttps://doi.org/10.2478/amb-2018-0016https://doaj.org/toc/0324-1750The endodontic treatment of immature permanent teeth with necrotic pulp is a serious clinical challenge. The chemical agents, used in regenerative procedures, should be selected not only based on their bactericidal/bacteriostatic properties, but also on their ability to ensure the survival of the patient’s stem cells. The aim of this study was to evaluate the effect of citric acid on the vitality of SCAP in a model of an immature tooth root. Models of immature roots were created from 12 freshly extracted teeth. The models were gas sterilized with ethylene oxide and they were separated into three groups, based on the used combinations of irrigants: 1) 1.5% sodium hypochlorite / 17% EDTA; 2) 1.5% sodium hypochlorite / 10% citric acid; 3) saline. SCAPs in a hyaluronic acid–based scaffold were seeded into the canals and cultured for 7 days. Viable cells were quantified using a colorimetric assay. There was no statistically significant difference between the groups, irrigated with NaOCl/EDTA and NaOCl/citric acid. The results from our experiment show that 10% citric acid can be used in combination with 1.5% NaOCl in a regenerative endodontic procedure.Hristov Kr.Gateva N.Stanimirov P.Ishkitiev N.Tsikandelova R.Mihaylova Z.Sciendoarticleapical papillastem cellscitric acidMedicineRENActa Medica Bulgarica, Vol 45, Iss 2, Pp 31-35 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	apical papilla stem cells citric acid Medicine R
spellingShingle	apical papilla stem cells citric acid Medicine R Hristov Kr. Gateva N. Stanimirov P. Ishkitiev N. Tsikandelova R. Mihaylova Z. Influence of Citric Acid on the Vitality of Stem Cells from Apical Papilla
description	The endodontic treatment of immature permanent teeth with necrotic pulp is a serious clinical challenge. The chemical agents, used in regenerative procedures, should be selected not only based on their bactericidal/bacteriostatic properties, but also on their ability to ensure the survival of the patient’s stem cells. The aim of this study was to evaluate the effect of citric acid on the vitality of SCAP in a model of an immature tooth root. Models of immature roots were created from 12 freshly extracted teeth. The models were gas sterilized with ethylene oxide and they were separated into three groups, based on the used combinations of irrigants: 1) 1.5% sodium hypochlorite / 17% EDTA; 2) 1.5% sodium hypochlorite / 10% citric acid; 3) saline. SCAPs in a hyaluronic acid–based scaffold were seeded into the canals and cultured for 7 days. Viable cells were quantified using a colorimetric assay. There was no statistically significant difference between the groups, irrigated with NaOCl/EDTA and NaOCl/citric acid. The results from our experiment show that 10% citric acid can be used in combination with 1.5% NaOCl in a regenerative endodontic procedure.
format	article
author	Hristov Kr. Gateva N. Stanimirov P. Ishkitiev N. Tsikandelova R. Mihaylova Z.
author_facet	Hristov Kr. Gateva N. Stanimirov P. Ishkitiev N. Tsikandelova R. Mihaylova Z.
author_sort	Hristov Kr.
title	Influence of Citric Acid on the Vitality of Stem Cells from Apical Papilla
title_short	Influence of Citric Acid on the Vitality of Stem Cells from Apical Papilla
title_full	Influence of Citric Acid on the Vitality of Stem Cells from Apical Papilla
title_fullStr	Influence of Citric Acid on the Vitality of Stem Cells from Apical Papilla
title_full_unstemmed	Influence of Citric Acid on the Vitality of Stem Cells from Apical Papilla
title_sort	influence of citric acid on the vitality of stem cells from apical papilla
publisher	Sciendo
publishDate	2018
url	https://doaj.org/article/d2660059c2ac41a59f6795cf5eb1f9df
work_keys_str_mv	AT hristovkr influenceofcitricacidonthevitalityofstemcellsfromapicalpapilla AT gatevan influenceofcitricacidonthevitalityofstemcellsfromapicalpapilla AT stanimirovp influenceofcitricacidonthevitalityofstemcellsfromapicalpapilla AT ishkitievn influenceofcitricacidonthevitalityofstemcellsfromapicalpapilla AT tsikandelovar influenceofcitricacidonthevitalityofstemcellsfromapicalpapilla AT mihaylovaz influenceofcitricacidonthevitalityofstemcellsfromapicalpapilla
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Influence of Citric Acid on the Vitality of Stem Cells from Apical Papilla

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