Gelatin Methacryloyl Hydrogels for the Localized Delivery of Cefazolin

The tuneability of hydrogels renders them promising candidates for local drug delivery to prevent and treat local surgical site infection (SSI) while avoiding the systemic side-effects of intravenous antibiotic injections. Here, we present a newly developed gelatin methacryloyl (GelMA)-based hydroge...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Margaux Vigata, Cathal D. O’Connell, Silvia Cometta, Dietmar W. Hutmacher, Christoph Meinert, Nathalie Bock
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	gelatin methacryloyl cefazolin localized antibiotic therapy drug delivery surgery site infection Organic chemistry QD241-441
Acceso en línea:	https://doaj.org/article/d26694bab5be413ba0119dddfdea4148
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:d26694bab5be413ba0119dddfdea4148
record_format	dspace
spelling	oai:doaj.org-article:d26694bab5be413ba0119dddfdea41482021-11-25T18:48:46ZGelatin Methacryloyl Hydrogels for the Localized Delivery of Cefazolin10.3390/polym132239602073-4360https://doaj.org/article/d26694bab5be413ba0119dddfdea41482021-11-01T00:00:00Zhttps://www.mdpi.com/2073-4360/13/22/3960https://doaj.org/toc/2073-4360The tuneability of hydrogels renders them promising candidates for local drug delivery to prevent and treat local surgical site infection (SSI) while avoiding the systemic side-effects of intravenous antibiotic injections. Here, we present a newly developed gelatin methacryloyl (GelMA)-based hydrogel drug delivery system (GelMA-DDS) to locally deliver the broad-spectrum antibiotic cefazolin for SSI prophylaxis and treatment. Antibiotic doses from 3 µg to 90 µg were loaded in photocrosslinked GelMA hydrogel discs with 5 to 15% <i>w/v</i> polymer concentration and drug encapsulation efficiencies, mechanical properties, crosslinking and release kinetics, as well as bacterial growth inhibition were assessed. Our results demonstrate that all GelMA groups supported excellent drug encapsulation efficiencies of up to 99%. Mechanical properties of the GelMA-DDS were highly tuneable and unaffected by the loading of small to medium doses of cefazolin. The diffusive and the proteolytic in vitro drug delivery of all investigated cefazolin doses was characterized by a burst release, and the delivered cefazolin amount was directly proportional to the encapsulated dose. Accelerated enzymatic degradation of the GelMA-DDS followed zero-order kinetics and was dependent on both the cefazolin dose and GelMA concentration (3–13 h). Finally, we demonstrate that cefazolin delivered from GelMA induced a dose-dependent antibacterial efficacy against <i>S. aureus</i>, in both a broth and a diffusive assay. The cefazolin-loaded GelMA-DDS presented here provides a highly tuneable and easy-to-use local delivery system for the prophylaxis and treatment of SSI.Margaux VigataCathal D. O’ConnellSilvia ComettaDietmar W. HutmacherChristoph MeinertNathalie BockMDPI AGarticlegelatin methacryloylcefazolinlocalized antibiotic therapydrug deliverysurgery site infectionOrganic chemistryQD241-441ENPolymers, Vol 13, Iss 3960, p 3960 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	gelatin methacryloyl cefazolin localized antibiotic therapy drug delivery surgery site infection Organic chemistry QD241-441
spellingShingle	gelatin methacryloyl cefazolin localized antibiotic therapy drug delivery surgery site infection Organic chemistry QD241-441 Margaux Vigata Cathal D. O’Connell Silvia Cometta Dietmar W. Hutmacher Christoph Meinert Nathalie Bock Gelatin Methacryloyl Hydrogels for the Localized Delivery of Cefazolin
description	The tuneability of hydrogels renders them promising candidates for local drug delivery to prevent and treat local surgical site infection (SSI) while avoiding the systemic side-effects of intravenous antibiotic injections. Here, we present a newly developed gelatin methacryloyl (GelMA)-based hydrogel drug delivery system (GelMA-DDS) to locally deliver the broad-spectrum antibiotic cefazolin for SSI prophylaxis and treatment. Antibiotic doses from 3 µg to 90 µg were loaded in photocrosslinked GelMA hydrogel discs with 5 to 15% <i>w/v</i> polymer concentration and drug encapsulation efficiencies, mechanical properties, crosslinking and release kinetics, as well as bacterial growth inhibition were assessed. Our results demonstrate that all GelMA groups supported excellent drug encapsulation efficiencies of up to 99%. Mechanical properties of the GelMA-DDS were highly tuneable and unaffected by the loading of small to medium doses of cefazolin. The diffusive and the proteolytic in vitro drug delivery of all investigated cefazolin doses was characterized by a burst release, and the delivered cefazolin amount was directly proportional to the encapsulated dose. Accelerated enzymatic degradation of the GelMA-DDS followed zero-order kinetics and was dependent on both the cefazolin dose and GelMA concentration (3–13 h). Finally, we demonstrate that cefazolin delivered from GelMA induced a dose-dependent antibacterial efficacy against <i>S. aureus</i>, in both a broth and a diffusive assay. The cefazolin-loaded GelMA-DDS presented here provides a highly tuneable and easy-to-use local delivery system for the prophylaxis and treatment of SSI.
format	article
author	Margaux Vigata Cathal D. O’Connell Silvia Cometta Dietmar W. Hutmacher Christoph Meinert Nathalie Bock
author_facet	Margaux Vigata Cathal D. O’Connell Silvia Cometta Dietmar W. Hutmacher Christoph Meinert Nathalie Bock
author_sort	Margaux Vigata
title	Gelatin Methacryloyl Hydrogels for the Localized Delivery of Cefazolin
title_short	Gelatin Methacryloyl Hydrogels for the Localized Delivery of Cefazolin
title_full	Gelatin Methacryloyl Hydrogels for the Localized Delivery of Cefazolin
title_fullStr	Gelatin Methacryloyl Hydrogels for the Localized Delivery of Cefazolin
title_full_unstemmed	Gelatin Methacryloyl Hydrogels for the Localized Delivery of Cefazolin
title_sort	gelatin methacryloyl hydrogels for the localized delivery of cefazolin
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/d26694bab5be413ba0119dddfdea4148
work_keys_str_mv	AT margauxvigata gelatinmethacryloylhydrogelsforthelocalizeddeliveryofcefazolin AT cathaldoconnell gelatinmethacryloylhydrogelsforthelocalizeddeliveryofcefazolin AT silviacometta gelatinmethacryloylhydrogelsforthelocalizeddeliveryofcefazolin AT dietmarwhutmacher gelatinmethacryloylhydrogelsforthelocalizeddeliveryofcefazolin AT christophmeinert gelatinmethacryloylhydrogelsforthelocalizeddeliveryofcefazolin AT nathaliebock gelatinmethacryloylhydrogelsforthelocalizeddeliveryofcefazolin
_version_	1718410679278370816

Gelatin Methacryloyl Hydrogels for the Localized Delivery of Cefazolin

Ejemplares similares