Immunization of Pigs with Recombinant Plasmids Containing Genes of Ubiquitinated p30, p54 and CD2v Proteins of African Swine Fever Virus

Three recombinant plasmid constructs, expressing chimeric proteins containing human ubiquitin fused to an ectodomain of one of the potentially protective proteins (p30, p54 and CD2v) of the attenuated MK-200 strain of African swine fever virus (ASFV), were created as potential inductors of specific...

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Autores principales:	Imatdinov Almaz R, Kazakova Anna S, Šekler Milanko, Morozova Diana Yu, Lyska Valentina M, Titov Ilya A, Sevskikh Timofey, Sereda Alexey D
Formato:	article
Lenguaje:	EN
Publicado:	Sciendo 2020
Materias:	african swine fever recombinant plasmids immunogenicity protectivity ubiquitin Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/d268fd22d28c466485b13ec17cb6ea6c
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spelling	oai:doaj.org-article:d268fd22d28c466485b13ec17cb6ea6c2021-11-17T21:27:53ZImmunization of Pigs with Recombinant Plasmids Containing Genes of Ubiquitinated p30, p54 and CD2v Proteins of African Swine Fever Virus1820-744810.2478/acve-2020-0007https://doaj.org/article/d268fd22d28c466485b13ec17cb6ea6c2020-03-01T00:00:00Zhttps://doi.org/10.2478/acve-2020-0007https://doaj.org/toc/1820-7448Three recombinant plasmid constructs, expressing chimeric proteins containing human ubiquitin fused to an ectodomain of one of the potentially protective proteins (p30, p54 and CD2v) of the attenuated MK-200 strain of African swine fever virus (ASFV), were created as potential inductors of specific antiviral cellular immunity. Three-time immunization of pigs with the mixture of these plasmids led to the formation of virus-specific cytotoxic T-lymphocytes (CTL), but did not induce production of virus-specific antibodies. After challenge with the homologous parental virulent ASFV strain M-78 at a dose of 103 HAD50, all five animals (four immunized pigs and one naïve) fell between the 4th and 7th days post infection. The obtained results demonstrated that induction of CTL did not protect pigs against challenge with the virulent ASFV. Balanced activation of CTL and antibody-mediated cellular mechanisms should be investigated.Imatdinov Almaz RKazakova Anna SŠekler MilankoMorozova Diana YuLyska Valentina MTitov Ilya ASevskikh TimofeySereda Alexey DSciendoarticleafrican swine feverrecombinant plasmidsimmunogenicityprotectivityubiquitinVeterinary medicineSF600-1100ENActa Veterinaria, Vol 70, Iss 1, Pp 92-109 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	african swine fever recombinant plasmids immunogenicity protectivity ubiquitin Veterinary medicine SF600-1100
spellingShingle	african swine fever recombinant plasmids immunogenicity protectivity ubiquitin Veterinary medicine SF600-1100 Imatdinov Almaz R Kazakova Anna S Šekler Milanko Morozova Diana Yu Lyska Valentina M Titov Ilya A Sevskikh Timofey Sereda Alexey D Immunization of Pigs with Recombinant Plasmids Containing Genes of Ubiquitinated p30, p54 and CD2v Proteins of African Swine Fever Virus
description	Three recombinant plasmid constructs, expressing chimeric proteins containing human ubiquitin fused to an ectodomain of one of the potentially protective proteins (p30, p54 and CD2v) of the attenuated MK-200 strain of African swine fever virus (ASFV), were created as potential inductors of specific antiviral cellular immunity. Three-time immunization of pigs with the mixture of these plasmids led to the formation of virus-specific cytotoxic T-lymphocytes (CTL), but did not induce production of virus-specific antibodies. After challenge with the homologous parental virulent ASFV strain M-78 at a dose of 103 HAD50, all five animals (four immunized pigs and one naïve) fell between the 4th and 7th days post infection. The obtained results demonstrated that induction of CTL did not protect pigs against challenge with the virulent ASFV. Balanced activation of CTL and antibody-mediated cellular mechanisms should be investigated.
format	article
author	Imatdinov Almaz R Kazakova Anna S Šekler Milanko Morozova Diana Yu Lyska Valentina M Titov Ilya A Sevskikh Timofey Sereda Alexey D
author_facet	Imatdinov Almaz R Kazakova Anna S Šekler Milanko Morozova Diana Yu Lyska Valentina M Titov Ilya A Sevskikh Timofey Sereda Alexey D
author_sort	Imatdinov Almaz R
title	Immunization of Pigs with Recombinant Plasmids Containing Genes of Ubiquitinated p30, p54 and CD2v Proteins of African Swine Fever Virus
title_short	Immunization of Pigs with Recombinant Plasmids Containing Genes of Ubiquitinated p30, p54 and CD2v Proteins of African Swine Fever Virus
title_full	Immunization of Pigs with Recombinant Plasmids Containing Genes of Ubiquitinated p30, p54 and CD2v Proteins of African Swine Fever Virus
title_fullStr	Immunization of Pigs with Recombinant Plasmids Containing Genes of Ubiquitinated p30, p54 and CD2v Proteins of African Swine Fever Virus
title_full_unstemmed	Immunization of Pigs with Recombinant Plasmids Containing Genes of Ubiquitinated p30, p54 and CD2v Proteins of African Swine Fever Virus
title_sort	immunization of pigs with recombinant plasmids containing genes of ubiquitinated p30, p54 and cd2v proteins of african swine fever virus
publisher	Sciendo
publishDate	2020
url	https://doaj.org/article/d268fd22d28c466485b13ec17cb6ea6c
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Immunization of Pigs with Recombinant Plasmids Containing Genes of Ubiquitinated p30, p54 and CD2v Proteins of African Swine Fever Virus

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