First molecular-based detection of SARS-CoV-2 virus in the field-collected houseflies

Abstract This is the first report of SARS-CoV-2 detection on field-collected Musca domestica housefly surface and tissue samples using the high-sensitive PCR assay which suggests the possible insect-borne transmission. The study was conducted in Shiraz city, southern Iran, in May and Jun 2020. Adult...

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Autores principales:	Aboozar Soltani, Marzieh Jamalidoust, Amin Hosseinpour, Mozaffar Vahedi, Hadi Ashraf, Saeed Yousefinejad
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d26f70bf88e345469ff71f2eb7652cf5
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spelling	oai:doaj.org-article:d26f70bf88e345469ff71f2eb7652cf52021-12-02T16:14:47ZFirst molecular-based detection of SARS-CoV-2 virus in the field-collected houseflies10.1038/s41598-021-93439-72045-2322https://doaj.org/article/d26f70bf88e345469ff71f2eb7652cf52021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-93439-7https://doaj.org/toc/2045-2322Abstract This is the first report of SARS-CoV-2 detection on field-collected Musca domestica housefly surface and tissue samples using the high-sensitive PCR assay which suggests the possible insect-borne transmission. The study was conducted in Shiraz city, southern Iran, in May and Jun 2020. Adult flies were sampled at the outdoor areas of two hospitals treating COVID-19 patients. Fly samples were first washed twice to remove the insect surface attached to SARS-CoV-2 virions. After that, the disinfected fly samples were homogenized. Fly surface washout and homogenate samples were tested using Taq Man real-time PCR assay for the SARS-CoV-2 virus. In a total of 156 houseflies, 75% of samples from the body washout samples were positive for SARS-CoV-2. Strikingly, 37% of the homogenized specimens were positive for the SARS-CoV-2, suggesting the possible infection of the insects or uptake of the virion to the insect metabolism. The other possibility is the houseflies up took the blood or blood fluids of the patients and the RNA of the SARS-CoV-2 survived in the insect body without replicating. Our preliminary findings suggest that the houseflies could transmit SARS-CoV-2 as a mechanical or biological vector especially during the warm seasons while increasing the population and activity of houseflies.Aboozar SoltaniMarzieh JamalidoustAmin HosseinpourMozaffar VahediHadi AshrafSaeed YousefinejadNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-7 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Aboozar Soltani Marzieh Jamalidoust Amin Hosseinpour Mozaffar Vahedi Hadi Ashraf Saeed Yousefinejad First molecular-based detection of SARS-CoV-2 virus in the field-collected houseflies
description	Abstract This is the first report of SARS-CoV-2 detection on field-collected Musca domestica housefly surface and tissue samples using the high-sensitive PCR assay which suggests the possible insect-borne transmission. The study was conducted in Shiraz city, southern Iran, in May and Jun 2020. Adult flies were sampled at the outdoor areas of two hospitals treating COVID-19 patients. Fly samples were first washed twice to remove the insect surface attached to SARS-CoV-2 virions. After that, the disinfected fly samples were homogenized. Fly surface washout and homogenate samples were tested using Taq Man real-time PCR assay for the SARS-CoV-2 virus. In a total of 156 houseflies, 75% of samples from the body washout samples were positive for SARS-CoV-2. Strikingly, 37% of the homogenized specimens were positive for the SARS-CoV-2, suggesting the possible infection of the insects or uptake of the virion to the insect metabolism. The other possibility is the houseflies up took the blood or blood fluids of the patients and the RNA of the SARS-CoV-2 survived in the insect body without replicating. Our preliminary findings suggest that the houseflies could transmit SARS-CoV-2 as a mechanical or biological vector especially during the warm seasons while increasing the population and activity of houseflies.
format	article
author	Aboozar Soltani Marzieh Jamalidoust Amin Hosseinpour Mozaffar Vahedi Hadi Ashraf Saeed Yousefinejad
author_facet	Aboozar Soltani Marzieh Jamalidoust Amin Hosseinpour Mozaffar Vahedi Hadi Ashraf Saeed Yousefinejad
author_sort	Aboozar Soltani
title	First molecular-based detection of SARS-CoV-2 virus in the field-collected houseflies
title_short	First molecular-based detection of SARS-CoV-2 virus in the field-collected houseflies
title_full	First molecular-based detection of SARS-CoV-2 virus in the field-collected houseflies
title_fullStr	First molecular-based detection of SARS-CoV-2 virus in the field-collected houseflies
title_full_unstemmed	First molecular-based detection of SARS-CoV-2 virus in the field-collected houseflies
title_sort	first molecular-based detection of sars-cov-2 virus in the field-collected houseflies
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/d26f70bf88e345469ff71f2eb7652cf5
work_keys_str_mv	AT aboozarsoltani firstmolecularbaseddetectionofsarscov2virusinthefieldcollectedhouseflies AT marziehjamalidoust firstmolecularbaseddetectionofsarscov2virusinthefieldcollectedhouseflies AT aminhosseinpour firstmolecularbaseddetectionofsarscov2virusinthefieldcollectedhouseflies AT mozaffarvahedi firstmolecularbaseddetectionofsarscov2virusinthefieldcollectedhouseflies AT hadiashraf firstmolecularbaseddetectionofsarscov2virusinthefieldcollectedhouseflies AT saeedyousefinejad firstmolecularbaseddetectionofsarscov2virusinthefieldcollectedhouseflies
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First molecular-based detection of SARS-CoV-2 virus in the field-collected houseflies

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