Coenzyme Q10 reduces ethanol-induced apoptosis in corneal fibroblasts.

Dilute ethanol (EtOH) is a widely used agent to remove the corneal epithelium during the modern refractive surgery. The application of EtOH may cause the underlying corneal fibroblasts to undergo apoptosis. This study was designed to investigate the protective effect and potential mechanism of the r...

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Autores principales:	Chun-Chen Chen, Shiow-Wen Liou, Chi-Chih Chen, Wen-Chung Chen, Fung-Rong Hu, I-Jong Wang, Shing-Jong Lin
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d27696fd80734035ad035a45eb06be61
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spelling	oai:doaj.org-article:d27696fd80734035ad035a45eb06be612021-11-18T06:55:03ZCoenzyme Q10 reduces ethanol-induced apoptosis in corneal fibroblasts.1932-620310.1371/journal.pone.0019111https://doaj.org/article/d27696fd80734035ad035a45eb06be612011-04-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21556371/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Dilute ethanol (EtOH) is a widely used agent to remove the corneal epithelium during the modern refractive surgery. The application of EtOH may cause the underlying corneal fibroblasts to undergo apoptosis. This study was designed to investigate the protective effect and potential mechanism of the respiratory chain coenzyme Q(10) (CoQ(10)), an electron transporter of the mitochondrial respiratory chain and a ubiquitous free radical scavenger, against EtOH-induced apoptosis of corneal fibroblasts. Corneal fibroblasts were pretreated with CoQ(10) (10 µM) for 2 h, followed by exposure to different concentrations of EtOH (0.4, 2, 4, and 20%) for 20 s. After indicated incubation period (2-12 h), MTT assay was used to examine cell viability. Treated cells were further assessed by flow cytometry to identify apoptosis. Reactive oxygen species (ROS) and the change in mitochondrial membrane potential were assessed using dichlorodihydrofluorescein diacetate/2',7'-dichlorofluorescein (DCFH-DA/DCF) assays and flow-cytometric analysis of JC-1 staining, respectively. The activity and expression of caspases 2, 3, 8, and 9 were evaluated with a colorimetric assay and western blot analysis. We found that EtOH treatment significantly decreased the viability of corneal fibroblasts characterized by a higher percentage of apoptotic cells. CoQ(10) could antagonize the apoptosis inducing effect of EtOH. The inhibition of cell apoptosis by CoQ(10) was significant at 8 and 12 h after EtOH exposure. In EtOH-exposed corneal fibroblasts, CoQ(10) pretreatment significantly reduced mitochondrial depolarization and ROS production at 30, 60, 90, and 120 min and inhibited the activation and expression of caspases 2 and 3 at 2 h after EtOH exposure. In summary, pretreatment with CoQ(10) can inhibit mitochondrial depolarization, caspase activation, and cell apoptosis. These findings support the proposition that CoQ(10) plays an antiapoptotic role in corneal fibroblasts after ethanol exposure.Chun-Chen ChenShiow-Wen LiouChi-Chih ChenWen-Chung ChenFung-Rong HuI-Jong WangShing-Jong LinPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 4, p e19111 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Chun-Chen Chen Shiow-Wen Liou Chi-Chih Chen Wen-Chung Chen Fung-Rong Hu I-Jong Wang Shing-Jong Lin Coenzyme Q10 reduces ethanol-induced apoptosis in corneal fibroblasts.
description	Dilute ethanol (EtOH) is a widely used agent to remove the corneal epithelium during the modern refractive surgery. The application of EtOH may cause the underlying corneal fibroblasts to undergo apoptosis. This study was designed to investigate the protective effect and potential mechanism of the respiratory chain coenzyme Q(10) (CoQ(10)), an electron transporter of the mitochondrial respiratory chain and a ubiquitous free radical scavenger, against EtOH-induced apoptosis of corneal fibroblasts. Corneal fibroblasts were pretreated with CoQ(10) (10 µM) for 2 h, followed by exposure to different concentrations of EtOH (0.4, 2, 4, and 20%) for 20 s. After indicated incubation period (2-12 h), MTT assay was used to examine cell viability. Treated cells were further assessed by flow cytometry to identify apoptosis. Reactive oxygen species (ROS) and the change in mitochondrial membrane potential were assessed using dichlorodihydrofluorescein diacetate/2',7'-dichlorofluorescein (DCFH-DA/DCF) assays and flow-cytometric analysis of JC-1 staining, respectively. The activity and expression of caspases 2, 3, 8, and 9 were evaluated with a colorimetric assay and western blot analysis. We found that EtOH treatment significantly decreased the viability of corneal fibroblasts characterized by a higher percentage of apoptotic cells. CoQ(10) could antagonize the apoptosis inducing effect of EtOH. The inhibition of cell apoptosis by CoQ(10) was significant at 8 and 12 h after EtOH exposure. In EtOH-exposed corneal fibroblasts, CoQ(10) pretreatment significantly reduced mitochondrial depolarization and ROS production at 30, 60, 90, and 120 min and inhibited the activation and expression of caspases 2 and 3 at 2 h after EtOH exposure. In summary, pretreatment with CoQ(10) can inhibit mitochondrial depolarization, caspase activation, and cell apoptosis. These findings support the proposition that CoQ(10) plays an antiapoptotic role in corneal fibroblasts after ethanol exposure.
format	article
author	Chun-Chen Chen Shiow-Wen Liou Chi-Chih Chen Wen-Chung Chen Fung-Rong Hu I-Jong Wang Shing-Jong Lin
author_facet	Chun-Chen Chen Shiow-Wen Liou Chi-Chih Chen Wen-Chung Chen Fung-Rong Hu I-Jong Wang Shing-Jong Lin
author_sort	Chun-Chen Chen
title	Coenzyme Q10 reduces ethanol-induced apoptosis in corneal fibroblasts.
title_short	Coenzyme Q10 reduces ethanol-induced apoptosis in corneal fibroblasts.
title_full	Coenzyme Q10 reduces ethanol-induced apoptosis in corneal fibroblasts.
title_fullStr	Coenzyme Q10 reduces ethanol-induced apoptosis in corneal fibroblasts.
title_full_unstemmed	Coenzyme Q10 reduces ethanol-induced apoptosis in corneal fibroblasts.
title_sort	coenzyme q10 reduces ethanol-induced apoptosis in corneal fibroblasts.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/d27696fd80734035ad035a45eb06be61
work_keys_str_mv	AT chunchenchen coenzymeq10reducesethanolinducedapoptosisincornealfibroblasts AT shiowwenliou coenzymeq10reducesethanolinducedapoptosisincornealfibroblasts AT chichihchen coenzymeq10reducesethanolinducedapoptosisincornealfibroblasts AT wenchungchen coenzymeq10reducesethanolinducedapoptosisincornealfibroblasts AT fungronghu coenzymeq10reducesethanolinducedapoptosisincornealfibroblasts AT ijongwang coenzymeq10reducesethanolinducedapoptosisincornealfibroblasts AT shingjonglin coenzymeq10reducesethanolinducedapoptosisincornealfibroblasts
_version_	1718424258748612608

Coenzyme Q10 reduces ethanol-induced apoptosis in corneal fibroblasts.

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