Fast genetic mapping using insertion-deletion polymorphisms in Caenorhabditis elegans

Abstract Genetic mapping is used in forward genetics to narrow the list of candidate mutations and genes corresponding to the mutant phenotype of interest. Even with modern advances in biology such as efficient identification of candidate mutations by whole-genome sequencing, mapping remains critica...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Ho-Yon Hwang, Jiou Wang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d2844a571cd94935bd1e7f8c598e31bd
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:d2844a571cd94935bd1e7f8c598e31bd
record_format	dspace
spelling	oai:doaj.org-article:d2844a571cd94935bd1e7f8c598e31bd2021-12-02T16:53:12ZFast genetic mapping using insertion-deletion polymorphisms in Caenorhabditis elegans10.1038/s41598-021-90190-x2045-2322https://doaj.org/article/d2844a571cd94935bd1e7f8c598e31bd2021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-90190-xhttps://doaj.org/toc/2045-2322Abstract Genetic mapping is used in forward genetics to narrow the list of candidate mutations and genes corresponding to the mutant phenotype of interest. Even with modern advances in biology such as efficient identification of candidate mutations by whole-genome sequencing, mapping remains critical in pinpointing the responsible mutation. Here we describe a simple, fast, and affordable mapping toolkit that is particularly suitable for mapping in Caenorhabditis elegans. This mapping method uses insertion-deletion polymorphisms or indels that could be easily detected instead of single nucleotide polymorphisms in commonly used Hawaiian CB4856 mapping strain. The materials and methods were optimized so that mapping could be performed using tiny amount of genetic material without growing many large populations of mutants for DNA purification. We performed mapping of previously known and unknown mutations to show strengths and weaknesses of this method and to present examples of completed mapping. For situations where Hawaiian CB4856 is unsuitable, we provide an annotated list of indels as a basis for fast and easy mapping using other wild isolates. Finally, we provide rationale for using this mapping method over other alternatives as a part of a comprehensive strategy also involving whole-genome sequencing and other methods.Ho-Yon HwangJiou WangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ho-Yon Hwang Jiou Wang Fast genetic mapping using insertion-deletion polymorphisms in Caenorhabditis elegans
description	Abstract Genetic mapping is used in forward genetics to narrow the list of candidate mutations and genes corresponding to the mutant phenotype of interest. Even with modern advances in biology such as efficient identification of candidate mutations by whole-genome sequencing, mapping remains critical in pinpointing the responsible mutation. Here we describe a simple, fast, and affordable mapping toolkit that is particularly suitable for mapping in Caenorhabditis elegans. This mapping method uses insertion-deletion polymorphisms or indels that could be easily detected instead of single nucleotide polymorphisms in commonly used Hawaiian CB4856 mapping strain. The materials and methods were optimized so that mapping could be performed using tiny amount of genetic material without growing many large populations of mutants for DNA purification. We performed mapping of previously known and unknown mutations to show strengths and weaknesses of this method and to present examples of completed mapping. For situations where Hawaiian CB4856 is unsuitable, we provide an annotated list of indels as a basis for fast and easy mapping using other wild isolates. Finally, we provide rationale for using this mapping method over other alternatives as a part of a comprehensive strategy also involving whole-genome sequencing and other methods.
format	article
author	Ho-Yon Hwang Jiou Wang
author_facet	Ho-Yon Hwang Jiou Wang
author_sort	Ho-Yon Hwang
title	Fast genetic mapping using insertion-deletion polymorphisms in Caenorhabditis elegans
title_short	Fast genetic mapping using insertion-deletion polymorphisms in Caenorhabditis elegans
title_full	Fast genetic mapping using insertion-deletion polymorphisms in Caenorhabditis elegans
title_fullStr	Fast genetic mapping using insertion-deletion polymorphisms in Caenorhabditis elegans
title_full_unstemmed	Fast genetic mapping using insertion-deletion polymorphisms in Caenorhabditis elegans
title_sort	fast genetic mapping using insertion-deletion polymorphisms in caenorhabditis elegans
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/d2844a571cd94935bd1e7f8c598e31bd
work_keys_str_mv	AT hoyonhwang fastgeneticmappingusinginsertiondeletionpolymorphismsincaenorhabditiselegans AT jiouwang fastgeneticmappingusinginsertiondeletionpolymorphismsincaenorhabditiselegans
_version_	1718382888485912576

Fast genetic mapping using insertion-deletion polymorphisms in Caenorhabditis elegans

Ejemplares similares