Shuang-Huang-Lian injection induces an immediate hypersensitivity reaction via C5a but not IgE

Abstract Among traditional Chinese medicine injections, intravenous Shuang-Huang-Lian (IV-SHL) has the highest incidence of injection-induced immediate hypersensitivity reactions (IHRs). The precise mechanisms of IV-SHL-induced IHRs remain ambiguous. In this study, we investigated the mechanisms of...

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Autores principales:	Yuan Gao, Rui Hou, Yixin Han, Qiaoling Fei, Runlan Cai, Yun Qi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d2848032da314aa5a6b768e7ee82c52c
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spelling	oai:doaj.org-article:d2848032da314aa5a6b768e7ee82c52c2021-12-02T12:33:00ZShuang-Huang-Lian injection induces an immediate hypersensitivity reaction via C5a but not IgE10.1038/s41598-018-21843-72045-2322https://doaj.org/article/d2848032da314aa5a6b768e7ee82c52c2018-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-21843-7https://doaj.org/toc/2045-2322Abstract Among traditional Chinese medicine injections, intravenous Shuang-Huang-Lian (IV-SHL) has the highest incidence of injection-induced immediate hypersensitivity reactions (IHRs). The precise mechanisms of IV-SHL-induced IHRs remain ambiguous. In this study, we investigated the mechanisms of SHL injection (SHLI)-induced IHRs. Our data showed that serum total IgE and mouse mast cell protease 1 (MMCP1) levels were higher in the SHLI antiserum; however, these effects of SHLI disappeared in the antibiotic-treated mice. SHLI caused intraplantar vasopermeability and shock during the first local or systemic injection. SHLI-induced nonallergic IHRs were attributed to its intermediate fraction F2 (the extract of Lonicerae Japonicae Flos and Fructus forsythiae), and could be blocked by antagonists for histamine or C5a, rather than PAF or C3a. Eight constituents of F2 were able to directly activate C5 to promote local vasopermeability at the mg/mL level. In conclusion, SHLI-induced IHRs are not mediated by IgE. SHLI or its F2 can directly activate blood C5. Subsequently, C5a is likely to provoke histamine release from its effector cells (e.g., mast cells and basophils), indicating that histamine is a principal effector of IHRs induced by SHLI.Yuan GaoRui HouYixin HanQiaoling FeiRunlan CaiYun QiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-10 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yuan Gao Rui Hou Yixin Han Qiaoling Fei Runlan Cai Yun Qi Shuang-Huang-Lian injection induces an immediate hypersensitivity reaction via C5a but not IgE
description	Abstract Among traditional Chinese medicine injections, intravenous Shuang-Huang-Lian (IV-SHL) has the highest incidence of injection-induced immediate hypersensitivity reactions (IHRs). The precise mechanisms of IV-SHL-induced IHRs remain ambiguous. In this study, we investigated the mechanisms of SHL injection (SHLI)-induced IHRs. Our data showed that serum total IgE and mouse mast cell protease 1 (MMCP1) levels were higher in the SHLI antiserum; however, these effects of SHLI disappeared in the antibiotic-treated mice. SHLI caused intraplantar vasopermeability and shock during the first local or systemic injection. SHLI-induced nonallergic IHRs were attributed to its intermediate fraction F2 (the extract of Lonicerae Japonicae Flos and Fructus forsythiae), and could be blocked by antagonists for histamine or C5a, rather than PAF or C3a. Eight constituents of F2 were able to directly activate C5 to promote local vasopermeability at the mg/mL level. In conclusion, SHLI-induced IHRs are not mediated by IgE. SHLI or its F2 can directly activate blood C5. Subsequently, C5a is likely to provoke histamine release from its effector cells (e.g., mast cells and basophils), indicating that histamine is a principal effector of IHRs induced by SHLI.
format	article
author	Yuan Gao Rui Hou Yixin Han Qiaoling Fei Runlan Cai Yun Qi
author_facet	Yuan Gao Rui Hou Yixin Han Qiaoling Fei Runlan Cai Yun Qi
author_sort	Yuan Gao
title	Shuang-Huang-Lian injection induces an immediate hypersensitivity reaction via C5a but not IgE
title_short	Shuang-Huang-Lian injection induces an immediate hypersensitivity reaction via C5a but not IgE
title_full	Shuang-Huang-Lian injection induces an immediate hypersensitivity reaction via C5a but not IgE
title_fullStr	Shuang-Huang-Lian injection induces an immediate hypersensitivity reaction via C5a but not IgE
title_full_unstemmed	Shuang-Huang-Lian injection induces an immediate hypersensitivity reaction via C5a but not IgE
title_sort	shuang-huang-lian injection induces an immediate hypersensitivity reaction via c5a but not ige
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/d2848032da314aa5a6b768e7ee82c52c
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Shuang-Huang-Lian injection induces an immediate hypersensitivity reaction via C5a but not IgE

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