Both OsRecQ1 and OsRDR1 are required for the production of small RNA in response to DNA-damage in rice.

Small RNA-mediated gene silencing pathways play important roles in the regulation of development, genome stability and various stress responses in many eukaryotes. Recently, a new type of small interfering RNAs (qiRNAs) approximately 20-21 nucleotides long in Neurospora crassa have been shown to med...

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Autores principales:	Hui Chen, Kappei Kobayashi, Akio Miyao, Hirohiko Hirochika, Naoto Yamaoka, Masamichi Nishiguchi
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d2997d587b1e460ab20bc438f0ba39d6
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spelling	oai:doaj.org-article:d2997d587b1e460ab20bc438f0ba39d62021-11-18T07:59:26ZBoth OsRecQ1 and OsRDR1 are required for the production of small RNA in response to DNA-damage in rice.1932-620310.1371/journal.pone.0055252https://doaj.org/article/d2997d587b1e460ab20bc438f0ba39d62013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23383126/?tool=EBIhttps://doaj.org/toc/1932-6203Small RNA-mediated gene silencing pathways play important roles in the regulation of development, genome stability and various stress responses in many eukaryotes. Recently, a new type of small interfering RNAs (qiRNAs) approximately 20-21 nucleotides long in Neurospora crassa have been shown to mediate gene silencing in the DNA damage response (DDR) pathway. However, the mechanism for RNA silencing in the DDR pathway is largely unknown in plants. Here, we report that a class of small RNAs (qiRNAs) derived from rDNA was markedly induced after treatment by DNA-damaging agents [ethyl methanesulphonate (EMS and UV-C)], and that aberrant RNAs (aRNAs) as precursors were also highly induced after the DNA damage treatment in rice. However, these RNAs were completely abolished in OsRecQ1 (RecQ DNA helicase homologue) and OsRDR1 (RNA-dependent RNA polymerase homologue) mutant lines where either gene was disrupted by the insertion of rice retrotransposon Tos17 after the same treatment. DNA damage resulted in a more significant increase in cell death and a more severe inhibition of root growth in both mutant lines than in the WT. Together, these results strongly suggest that both OsRecQ1 and OsRDR1 play a pivotal role in the aRNA and qiRNA biogenesis required for the DDR and repair pathway in rice, and it may be a novel mechanism of regulation to the DDR through the production of qiRNA in plants.Hui ChenKappei KobayashiAkio MiyaoHirohiko HirochikaNaoto YamaokaMasamichi NishiguchiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 1, p e55252 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hui Chen Kappei Kobayashi Akio Miyao Hirohiko Hirochika Naoto Yamaoka Masamichi Nishiguchi Both OsRecQ1 and OsRDR1 are required for the production of small RNA in response to DNA-damage in rice.
description	Small RNA-mediated gene silencing pathways play important roles in the regulation of development, genome stability and various stress responses in many eukaryotes. Recently, a new type of small interfering RNAs (qiRNAs) approximately 20-21 nucleotides long in Neurospora crassa have been shown to mediate gene silencing in the DNA damage response (DDR) pathway. However, the mechanism for RNA silencing in the DDR pathway is largely unknown in plants. Here, we report that a class of small RNAs (qiRNAs) derived from rDNA was markedly induced after treatment by DNA-damaging agents [ethyl methanesulphonate (EMS and UV-C)], and that aberrant RNAs (aRNAs) as precursors were also highly induced after the DNA damage treatment in rice. However, these RNAs were completely abolished in OsRecQ1 (RecQ DNA helicase homologue) and OsRDR1 (RNA-dependent RNA polymerase homologue) mutant lines where either gene was disrupted by the insertion of rice retrotransposon Tos17 after the same treatment. DNA damage resulted in a more significant increase in cell death and a more severe inhibition of root growth in both mutant lines than in the WT. Together, these results strongly suggest that both OsRecQ1 and OsRDR1 play a pivotal role in the aRNA and qiRNA biogenesis required for the DDR and repair pathway in rice, and it may be a novel mechanism of regulation to the DDR through the production of qiRNA in plants.
format	article
author	Hui Chen Kappei Kobayashi Akio Miyao Hirohiko Hirochika Naoto Yamaoka Masamichi Nishiguchi
author_facet	Hui Chen Kappei Kobayashi Akio Miyao Hirohiko Hirochika Naoto Yamaoka Masamichi Nishiguchi
author_sort	Hui Chen
title	Both OsRecQ1 and OsRDR1 are required for the production of small RNA in response to DNA-damage in rice.
title_short	Both OsRecQ1 and OsRDR1 are required for the production of small RNA in response to DNA-damage in rice.
title_full	Both OsRecQ1 and OsRDR1 are required for the production of small RNA in response to DNA-damage in rice.
title_fullStr	Both OsRecQ1 and OsRDR1 are required for the production of small RNA in response to DNA-damage in rice.
title_full_unstemmed	Both OsRecQ1 and OsRDR1 are required for the production of small RNA in response to DNA-damage in rice.
title_sort	both osrecq1 and osrdr1 are required for the production of small rna in response to dna-damage in rice.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/d2997d587b1e460ab20bc438f0ba39d6
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Both OsRecQ1 and OsRDR1 are required for the production of small RNA in response to DNA-damage in rice.

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