Immunomodulatory effects of mesenchymal stem cell-conditioned media on lipopolysaccharide of Vibrio cholerae as a vaccine candidate

Abstract Background Vibrio cholerae is the causative agent of cholera, which is commonly associated with high morbidity and mortality, and presents a major challenge to healthcare systems throughout the world. Lipopolysaccharide (LPS) is required for full protection against V. cholerae but can induc...

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Autores principales:	Mahboube Bahroudi, Bita Bakhshi, Sara Soudi, Shahin Najar-peerayeh
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	LPS Vibrio cholerae Mesenchymal stem cells Cytokine Antibody Medicine (General) R5-920 Biochemistry QD415-436
Acceso en línea:	https://doaj.org/article/d2a136b9d3f849d7b1b0e3851ad75473
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spelling	oai:doaj.org-article:d2a136b9d3f849d7b1b0e3851ad754732021-11-07T12:07:32ZImmunomodulatory effects of mesenchymal stem cell-conditioned media on lipopolysaccharide of Vibrio cholerae as a vaccine candidate10.1186/s13287-021-02622-01757-6512https://doaj.org/article/d2a136b9d3f849d7b1b0e3851ad754732021-11-01T00:00:00Zhttps://doi.org/10.1186/s13287-021-02622-0https://doaj.org/toc/1757-6512Abstract Background Vibrio cholerae is the causative agent of cholera, which is commonly associated with high morbidity and mortality, and presents a major challenge to healthcare systems throughout the world. Lipopolysaccharide (LPS) is required for full protection against V. cholerae but can induce inflammation and septic shock. Mesenchymal stem cells (MSCs) are currently used to treat infectious and inflammatory diseases. Therefore, this study aimed to evaluate the immune-modulating effects of the LPS‐MSC‐conditioned medium (CM) on V. cholerae LPS immunization in a murine model. Methods After preconditioning MSCs with LPS, mice were immunized intraperitoneally on days 0 and 14 with the following combinations: LPS + LPS-MSC-CM; detoxified LPS (DLPS) + MSC-CM; LPS + MSC sup; LPS; LPS-MSC-CM; MSC supernatant (MSC sup); and PBS. The mouse serum and saliva samples were collected to evaluate antibody (serum IgG and saliva IgA) and cytokine responses (TNF-α, IL-10, IL-6, TGF-β, IL-4, IL-5, and B-cell activating factor (BAFF)). Results The LPS + LPS-MSC-CM significantly increased total IgG and IgA compared to other combinations (P < 0.001). TNF-α levels, in contrast to IL-10 and TGF-β, were reduced significantly in mice receiving the LPS + LPS-MSC-CM compared to mice receiving only LPS. IL-4, IL-5, and BAFF levels significantly increased in mice receiving increased doses of LPS + LPS-MSC-CM compared to those who received only LPS. The highest vibriocidal antibody titer (1:64) was observed in LPS + LPS-MSC-CM-immunized mice and resulted in a significant improvement in survival in infant mice infected by V. cholerae O1. Conclusions The LPS-MSC-CM modulates the immune response to V. cholerae LPS by regulating inflammatory and anti-inflammatory responses and inducing vibriocidal antibodies, which protect neonate mice against V. cholerae infection.Mahboube BahroudiBita BakhshiSara SoudiShahin Najar-peerayehBMCarticleLPSVibrio choleraeMesenchymal stem cellsCytokineAntibodyMedicine (General)R5-920BiochemistryQD415-436ENStem Cell Research & Therapy, Vol 12, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	LPS Vibrio cholerae Mesenchymal stem cells Cytokine Antibody Medicine (General) R5-920 Biochemistry QD415-436
spellingShingle	LPS Vibrio cholerae Mesenchymal stem cells Cytokine Antibody Medicine (General) R5-920 Biochemistry QD415-436 Mahboube Bahroudi Bita Bakhshi Sara Soudi Shahin Najar-peerayeh Immunomodulatory effects of mesenchymal stem cell-conditioned media on lipopolysaccharide of Vibrio cholerae as a vaccine candidate
description	Abstract Background Vibrio cholerae is the causative agent of cholera, which is commonly associated with high morbidity and mortality, and presents a major challenge to healthcare systems throughout the world. Lipopolysaccharide (LPS) is required for full protection against V. cholerae but can induce inflammation and septic shock. Mesenchymal stem cells (MSCs) are currently used to treat infectious and inflammatory diseases. Therefore, this study aimed to evaluate the immune-modulating effects of the LPS‐MSC‐conditioned medium (CM) on V. cholerae LPS immunization in a murine model. Methods After preconditioning MSCs with LPS, mice were immunized intraperitoneally on days 0 and 14 with the following combinations: LPS + LPS-MSC-CM; detoxified LPS (DLPS) + MSC-CM; LPS + MSC sup; LPS; LPS-MSC-CM; MSC supernatant (MSC sup); and PBS. The mouse serum and saliva samples were collected to evaluate antibody (serum IgG and saliva IgA) and cytokine responses (TNF-α, IL-10, IL-6, TGF-β, IL-4, IL-5, and B-cell activating factor (BAFF)). Results The LPS + LPS-MSC-CM significantly increased total IgG and IgA compared to other combinations (P < 0.001). TNF-α levels, in contrast to IL-10 and TGF-β, were reduced significantly in mice receiving the LPS + LPS-MSC-CM compared to mice receiving only LPS. IL-4, IL-5, and BAFF levels significantly increased in mice receiving increased doses of LPS + LPS-MSC-CM compared to those who received only LPS. The highest vibriocidal antibody titer (1:64) was observed in LPS + LPS-MSC-CM-immunized mice and resulted in a significant improvement in survival in infant mice infected by V. cholerae O1. Conclusions The LPS-MSC-CM modulates the immune response to V. cholerae LPS by regulating inflammatory and anti-inflammatory responses and inducing vibriocidal antibodies, which protect neonate mice against V. cholerae infection.
format	article
author	Mahboube Bahroudi Bita Bakhshi Sara Soudi Shahin Najar-peerayeh
author_facet	Mahboube Bahroudi Bita Bakhshi Sara Soudi Shahin Najar-peerayeh
author_sort	Mahboube Bahroudi
title	Immunomodulatory effects of mesenchymal stem cell-conditioned media on lipopolysaccharide of Vibrio cholerae as a vaccine candidate
title_short	Immunomodulatory effects of mesenchymal stem cell-conditioned media on lipopolysaccharide of Vibrio cholerae as a vaccine candidate
title_full	Immunomodulatory effects of mesenchymal stem cell-conditioned media on lipopolysaccharide of Vibrio cholerae as a vaccine candidate
title_fullStr	Immunomodulatory effects of mesenchymal stem cell-conditioned media on lipopolysaccharide of Vibrio cholerae as a vaccine candidate
title_full_unstemmed	Immunomodulatory effects of mesenchymal stem cell-conditioned media on lipopolysaccharide of Vibrio cholerae as a vaccine candidate
title_sort	immunomodulatory effects of mesenchymal stem cell-conditioned media on lipopolysaccharide of vibrio cholerae as a vaccine candidate
publisher	BMC
publishDate	2021
url	https://doaj.org/article/d2a136b9d3f849d7b1b0e3851ad75473
work_keys_str_mv	AT mahboubebahroudi immunomodulatoryeffectsofmesenchymalstemcellconditionedmediaonlipopolysaccharideofvibriocholeraeasavaccinecandidate AT bitabakhshi immunomodulatoryeffectsofmesenchymalstemcellconditionedmediaonlipopolysaccharideofvibriocholeraeasavaccinecandidate AT sarasoudi immunomodulatoryeffectsofmesenchymalstemcellconditionedmediaonlipopolysaccharideofvibriocholeraeasavaccinecandidate AT shahinnajarpeerayeh immunomodulatoryeffectsofmesenchymalstemcellconditionedmediaonlipopolysaccharideofvibriocholeraeasavaccinecandidate
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Immunomodulatory effects of mesenchymal stem cell-conditioned media on lipopolysaccharide of Vibrio cholerae as a vaccine candidate

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