Hapten synthesis and production of specific antibody against 3-amino-5-morpholinomethyl-2-oxazolidone for immunoassay without derivatisation

Immunoassay direct for 3-amino-5-morpholinomethyl-2-oxazolidone (AMOZ), the metabolite of furaltadone, based on a specific antibody was usually unavailable due to its small molecule weight. In this work, a hapten with a double bond and an active carboxyl group was designed and then conjugated to pep...

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Autores principales:	Fengyin Liu, Zijian Chen, Yudong Shen, Yuanming Sun, Jinyi Yang, Hong Wang, Hongtao Lei, Zhenlin Xu
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2018
Materias:	immunoassay 3-amino-5-morpholinomethyl-2-oxazolidone dendrimer multiple hapten Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/d2a4afa050884cc19bd7ad2f5557952a
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spelling	oai:doaj.org-article:d2a4afa050884cc19bd7ad2f5557952a2021-11-26T11:19:46ZHapten synthesis and production of specific antibody against 3-amino-5-morpholinomethyl-2-oxazolidone for immunoassay without derivatisation0954-01051465-344310.1080/09540105.2017.1376038https://doaj.org/article/d2a4afa050884cc19bd7ad2f5557952a2018-01-01T00:00:00Zhttp://dx.doi.org/10.1080/09540105.2017.1376038https://doaj.org/toc/0954-0105https://doaj.org/toc/1465-3443Immunoassay direct for 3-amino-5-morpholinomethyl-2-oxazolidone (AMOZ), the metabolite of furaltadone, based on a specific antibody was usually unavailable due to its small molecule weight. In this work, a hapten with a double bond and an active carboxyl group was designed and then conjugated to peptide dendrimer to obtain novel multiple haptens. The haptens and multiple haptens were coupled to bovine serum albumin as immunogens. Polyclonal antibodies showed binding to free AMOZ in a heterologous competitive indirect enzyme-linked immunosorbent assay were obtained. The assay showed IC50 (half maximal inhibitory concentration) of 4.1 μg/kg and limit of detection of 0.2 μg/kg for AMOZ. Recoveries of AMOZ from grass carp were tested from 83.1% to 117.0%, with the coefficient of variation below 12%. As no laborious derivatisation procedure was employed, the proposed assay should be useful for the screening of AMOZ for a large number of samples.Fengyin LiuZijian ChenYudong ShenYuanming SunJinyi YangHong WangHongtao LeiZhenlin XuTaylor & Francis Grouparticleimmunoassay3-amino-5-morpholinomethyl-2-oxazolidonedendrimermultiple haptenAgriculture (General)S1-972Immunologic diseases. AllergyRC581-607ENFood and Agricultural Immunology, Vol 29, Iss 1, Pp 332-345 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	immunoassay 3-amino-5-morpholinomethyl-2-oxazolidone dendrimer multiple hapten Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607
spellingShingle	immunoassay 3-amino-5-morpholinomethyl-2-oxazolidone dendrimer multiple hapten Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607 Fengyin Liu Zijian Chen Yudong Shen Yuanming Sun Jinyi Yang Hong Wang Hongtao Lei Zhenlin Xu Hapten synthesis and production of specific antibody against 3-amino-5-morpholinomethyl-2-oxazolidone for immunoassay without derivatisation
description	Immunoassay direct for 3-amino-5-morpholinomethyl-2-oxazolidone (AMOZ), the metabolite of furaltadone, based on a specific antibody was usually unavailable due to its small molecule weight. In this work, a hapten with a double bond and an active carboxyl group was designed and then conjugated to peptide dendrimer to obtain novel multiple haptens. The haptens and multiple haptens were coupled to bovine serum albumin as immunogens. Polyclonal antibodies showed binding to free AMOZ in a heterologous competitive indirect enzyme-linked immunosorbent assay were obtained. The assay showed IC50 (half maximal inhibitory concentration) of 4.1 μg/kg and limit of detection of 0.2 μg/kg for AMOZ. Recoveries of AMOZ from grass carp were tested from 83.1% to 117.0%, with the coefficient of variation below 12%. As no laborious derivatisation procedure was employed, the proposed assay should be useful for the screening of AMOZ for a large number of samples.
format	article
author	Fengyin Liu Zijian Chen Yudong Shen Yuanming Sun Jinyi Yang Hong Wang Hongtao Lei Zhenlin Xu
author_facet	Fengyin Liu Zijian Chen Yudong Shen Yuanming Sun Jinyi Yang Hong Wang Hongtao Lei Zhenlin Xu
author_sort	Fengyin Liu
title	Hapten synthesis and production of specific antibody against 3-amino-5-morpholinomethyl-2-oxazolidone for immunoassay without derivatisation
title_short	Hapten synthesis and production of specific antibody against 3-amino-5-morpholinomethyl-2-oxazolidone for immunoassay without derivatisation
title_full	Hapten synthesis and production of specific antibody against 3-amino-5-morpholinomethyl-2-oxazolidone for immunoassay without derivatisation
title_fullStr	Hapten synthesis and production of specific antibody against 3-amino-5-morpholinomethyl-2-oxazolidone for immunoassay without derivatisation
title_full_unstemmed	Hapten synthesis and production of specific antibody against 3-amino-5-morpholinomethyl-2-oxazolidone for immunoassay without derivatisation
title_sort	hapten synthesis and production of specific antibody against 3-amino-5-morpholinomethyl-2-oxazolidone for immunoassay without derivatisation
publisher	Taylor & Francis Group
publishDate	2018
url	https://doaj.org/article/d2a4afa050884cc19bd7ad2f5557952a
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Hapten synthesis and production of specific antibody against 3-amino-5-morpholinomethyl-2-oxazolidone for immunoassay without derivatisation

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