A New Clark-Type Layered Double Hydroxides-Enzyme Biosensor for H<sub>2</sub>O<sub>2</sub> Determination in Highly Diluted Real Matrices: Milk and Cosmetics
A new catalase amperometric biosensor for hydroperoxides detection has been built as part of research aimed at the development of biosensors based on layered double hydroxides (LDH) used as support for enzyme immobilization. The fabricated device differs from those developed so far, usually based on...
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MDPI AG
2021
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oai:doaj.org-article:d2a74874ad124f158ee957b7ce32205d2021-11-25T18:50:00ZA New Clark-Type Layered Double Hydroxides-Enzyme Biosensor for H<sub>2</sub>O<sub>2</sub> Determination in Highly Diluted Real Matrices: Milk and Cosmetics10.3390/pr91118782227-9717https://doaj.org/article/d2a74874ad124f158ee957b7ce32205d2021-10-01T00:00:00Zhttps://www.mdpi.com/2227-9717/9/11/1878https://doaj.org/toc/2227-9717A new catalase amperometric biosensor for hydroperoxides detection has been built as part of research aimed at the development of biosensors based on layered double hydroxides (LDH) used as support for enzyme immobilization. The fabricated device differs from those developed so far, usually based on an LDH enzyme nanocomposite adsorbed on a glassy carbon (GC) electrode and cross-linked by glutaraldehyde, since it is based on an amperometric gas diffusion electrode (Clark type) instead of a GC electrode. The new biosensor, which still uses LDH synthesized by us and catalase enzyme, is robust and compact, shows a lower LOD (limit of detection) value and a linearity range shifted at lower concentrations than direct amperometric GC biosensor, but above all, it is not affected by turbidity or emulsions, or by the presence of possible soluble species, which are reduced to the cathode at the same redox potential. This made it possible to carry out accurate and efficient determination of H<sub>2</sub>O<sub>2</sub> even in complex or cloudy real matrices, also containing very low concentrations of hydrogen peroxide, such as milk and cosmetic products, i.e., matrices that would have been impossible to analyze otherwise, using conventional biosensors based on a GC–LDH enzyme. An inaccuracy ≤ 7.7% for cosmetic samples and ≤8.0% for milk samples and a precision between 0.7 and 1.5 (as RSD%), according to cosmetic or milk samples analyzed, were achieved.Mauro TomassettiRiccardo PezzilliGiuseppe PrestopinoFrancesco Di BiagioCorrado Di NatalePier Gianni MedagliaMDPI AGarticleclark-type LDH-catalase biosensorH<sub>2</sub>O<sub>2</sub> determinationreal samplescow milk and cosmeticno interference effectsChemical technologyTP1-1185ChemistryQD1-999ENProcesses, Vol 9, Iss 1878, p 1878 (2021) |
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clark-type LDH-catalase biosensor H<sub>2</sub>O<sub>2</sub> determination real samples cow milk and cosmetic no interference effects Chemical technology TP1-1185 Chemistry QD1-999 |
| spellingShingle |
clark-type LDH-catalase biosensor H<sub>2</sub>O<sub>2</sub> determination real samples cow milk and cosmetic no interference effects Chemical technology TP1-1185 Chemistry QD1-999 Mauro Tomassetti Riccardo Pezzilli Giuseppe Prestopino Francesco Di Biagio Corrado Di Natale Pier Gianni Medaglia A New Clark-Type Layered Double Hydroxides-Enzyme Biosensor for H<sub>2</sub>O<sub>2</sub> Determination in Highly Diluted Real Matrices: Milk and Cosmetics |
| description |
A new catalase amperometric biosensor for hydroperoxides detection has been built as part of research aimed at the development of biosensors based on layered double hydroxides (LDH) used as support for enzyme immobilization. The fabricated device differs from those developed so far, usually based on an LDH enzyme nanocomposite adsorbed on a glassy carbon (GC) electrode and cross-linked by glutaraldehyde, since it is based on an amperometric gas diffusion electrode (Clark type) instead of a GC electrode. The new biosensor, which still uses LDH synthesized by us and catalase enzyme, is robust and compact, shows a lower LOD (limit of detection) value and a linearity range shifted at lower concentrations than direct amperometric GC biosensor, but above all, it is not affected by turbidity or emulsions, or by the presence of possible soluble species, which are reduced to the cathode at the same redox potential. This made it possible to carry out accurate and efficient determination of H<sub>2</sub>O<sub>2</sub> even in complex or cloudy real matrices, also containing very low concentrations of hydrogen peroxide, such as milk and cosmetic products, i.e., matrices that would have been impossible to analyze otherwise, using conventional biosensors based on a GC–LDH enzyme. An inaccuracy ≤ 7.7% for cosmetic samples and ≤8.0% for milk samples and a precision between 0.7 and 1.5 (as RSD%), according to cosmetic or milk samples analyzed, were achieved. |
| format |
article |
| author |
Mauro Tomassetti Riccardo Pezzilli Giuseppe Prestopino Francesco Di Biagio Corrado Di Natale Pier Gianni Medaglia |
| author_facet |
Mauro Tomassetti Riccardo Pezzilli Giuseppe Prestopino Francesco Di Biagio Corrado Di Natale Pier Gianni Medaglia |
| author_sort |
Mauro Tomassetti |
| title |
A New Clark-Type Layered Double Hydroxides-Enzyme Biosensor for H<sub>2</sub>O<sub>2</sub> Determination in Highly Diluted Real Matrices: Milk and Cosmetics |
| title_short |
A New Clark-Type Layered Double Hydroxides-Enzyme Biosensor for H<sub>2</sub>O<sub>2</sub> Determination in Highly Diluted Real Matrices: Milk and Cosmetics |
| title_full |
A New Clark-Type Layered Double Hydroxides-Enzyme Biosensor for H<sub>2</sub>O<sub>2</sub> Determination in Highly Diluted Real Matrices: Milk and Cosmetics |
| title_fullStr |
A New Clark-Type Layered Double Hydroxides-Enzyme Biosensor for H<sub>2</sub>O<sub>2</sub> Determination in Highly Diluted Real Matrices: Milk and Cosmetics |
| title_full_unstemmed |
A New Clark-Type Layered Double Hydroxides-Enzyme Biosensor for H<sub>2</sub>O<sub>2</sub> Determination in Highly Diluted Real Matrices: Milk and Cosmetics |
| title_sort |
new clark-type layered double hydroxides-enzyme biosensor for h<sub>2</sub>o<sub>2</sub> determination in highly diluted real matrices: milk and cosmetics |
| publisher |
MDPI AG |
| publishDate |
2021 |
| url |
https://doaj.org/article/d2a74874ad124f158ee957b7ce32205d |
| work_keys_str_mv |
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| _version_ |
1718410679660052480 |