Use of electromagnetic stimulation on an Enterococcus faecalis biofilm on root canal treated teeth in vitro

Abstract Root canal disinfection is of utmost importance in the success of the treatment, thus, a novel method for achieving root canal disinfection by electromagnetic waves, creating a synergistic reaction via electric and thermal energy, was created. To study electromagnetic stimulation (EMS) for...

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Autores principales:	Beatriz H. D. Panariello, Justin K. Kindler, Kenneth J. Spolnik, Ygal Ehrlich, George J. Eckert, Simone Duarte
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d2b2abe8c7c249bcacd530b663d4ad32
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spelling	oai:doaj.org-article:d2b2abe8c7c249bcacd530b663d4ad322021-12-02T14:26:20ZUse of electromagnetic stimulation on an Enterococcus faecalis biofilm on root canal treated teeth in vitro10.1038/s41598-021-87922-42045-2322https://doaj.org/article/d2b2abe8c7c249bcacd530b663d4ad322021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-87922-4https://doaj.org/toc/2045-2322Abstract Root canal disinfection is of utmost importance in the success of the treatment, thus, a novel method for achieving root canal disinfection by electromagnetic waves, creating a synergistic reaction via electric and thermal energy, was created. To study electromagnetic stimulation (EMS) for the disinfection of root canal in vitro, single rooted teeth were instrumented with a 45.05 Wave One Gold reciprocating file. Specimens were sterilized and inoculated with Enterococcus faecalis ATCC 29,212, which grew for 15 days to form an established biofilm. Samples were treated with 6% sodium hypochlorite (NaOCl), 1.5% NaOCl 1.5% NaOCl with EMS, 0.9% saline with EMS or 0.9% saline. After treatments, the colony forming units (CFU) was determined. Data was analyzed by Wilcoxon Rank Sums Test (α = 0.05). One sample per group was scored and split for confocal laser scanning microscopy imaging. There was a significant effect with the use of NaOCl with or without EMS versus 0.9% saline with or without EMS (p = 0.012 and 0.003, respectively). CFUs were lower when using 0.9% saline with EMS versus 0.9% saline alone (p = 0.002). Confocal imaging confirmed CFU findings. EMS with saline has an antibiofilm effect against E. faecalis and can potentially be applied for endodontic disinfection.Beatriz H. D. PanarielloJustin K. KindlerKenneth J. SpolnikYgal EhrlichGeorge J. EckertSimone DuarteNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Beatriz H. D. Panariello Justin K. Kindler Kenneth J. Spolnik Ygal Ehrlich George J. Eckert Simone Duarte Use of electromagnetic stimulation on an Enterococcus faecalis biofilm on root canal treated teeth in vitro
description	Abstract Root canal disinfection is of utmost importance in the success of the treatment, thus, a novel method for achieving root canal disinfection by electromagnetic waves, creating a synergistic reaction via electric and thermal energy, was created. To study electromagnetic stimulation (EMS) for the disinfection of root canal in vitro, single rooted teeth were instrumented with a 45.05 Wave One Gold reciprocating file. Specimens were sterilized and inoculated with Enterococcus faecalis ATCC 29,212, which grew for 15 days to form an established biofilm. Samples were treated with 6% sodium hypochlorite (NaOCl), 1.5% NaOCl 1.5% NaOCl with EMS, 0.9% saline with EMS or 0.9% saline. After treatments, the colony forming units (CFU) was determined. Data was analyzed by Wilcoxon Rank Sums Test (α = 0.05). One sample per group was scored and split for confocal laser scanning microscopy imaging. There was a significant effect with the use of NaOCl with or without EMS versus 0.9% saline with or without EMS (p = 0.012 and 0.003, respectively). CFUs were lower when using 0.9% saline with EMS versus 0.9% saline alone (p = 0.002). Confocal imaging confirmed CFU findings. EMS with saline has an antibiofilm effect against E. faecalis and can potentially be applied for endodontic disinfection.
format	article
author	Beatriz H. D. Panariello Justin K. Kindler Kenneth J. Spolnik Ygal Ehrlich George J. Eckert Simone Duarte
author_facet	Beatriz H. D. Panariello Justin K. Kindler Kenneth J. Spolnik Ygal Ehrlich George J. Eckert Simone Duarte
author_sort	Beatriz H. D. Panariello
title	Use of electromagnetic stimulation on an Enterococcus faecalis biofilm on root canal treated teeth in vitro
title_short	Use of electromagnetic stimulation on an Enterococcus faecalis biofilm on root canal treated teeth in vitro
title_full	Use of electromagnetic stimulation on an Enterococcus faecalis biofilm on root canal treated teeth in vitro
title_fullStr	Use of electromagnetic stimulation on an Enterococcus faecalis biofilm on root canal treated teeth in vitro
title_full_unstemmed	Use of electromagnetic stimulation on an Enterococcus faecalis biofilm on root canal treated teeth in vitro
title_sort	use of electromagnetic stimulation on an enterococcus faecalis biofilm on root canal treated teeth in vitro
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/d2b2abe8c7c249bcacd530b663d4ad32
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Use of electromagnetic stimulation on an Enterococcus faecalis biofilm on root canal treated teeth in vitro

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