Stem cell-derived polarized hepatocytes

To model hepatocyte function accurately in vitro, it is necessary to generate and maintain a polarized epithelium. Here, the authors describe a protocol to generate polarized human pluripotent stem cell-derived hepatocyte-like cells (HLCs) to model enteric virus production and drug secretion in vitr...

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Autores principales:	Viet Loan Dao Thi, Xianfang Wu, Rachel L. Belote, Ursula Andreo, Constantin N. Takacs, Joseph P. Fernandez, Luis Andre Vale-Silva, Sarah Prallet, Charlotte C. Decker, Rebecca M. Fu, Bingqian Qu, Kunihiro Uryu, Henrik Molina, Mohsan Saeed, Eike Steinmann, Stephan Urban, Roshni R. Singaraja, William M. Schneider, Sanford M. Simon, Charles M. Rice
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/d2bbfc7838fb493fa97035285b6d1783
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spelling	oai:doaj.org-article:d2bbfc7838fb493fa97035285b6d17832021-12-02T17:31:56ZStem cell-derived polarized hepatocytes10.1038/s41467-020-15337-22041-1723https://doaj.org/article/d2bbfc7838fb493fa97035285b6d17832020-04-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-15337-2https://doaj.org/toc/2041-1723To model hepatocyte function accurately in vitro, it is necessary to generate and maintain a polarized epithelium. Here, the authors describe a protocol to generate polarized human pluripotent stem cell-derived hepatocyte-like cells (HLCs) to model enteric virus production and drug secretion in vitro.Viet Loan Dao ThiXianfang WuRachel L. BeloteUrsula AndreoConstantin N. TakacsJoseph P. FernandezLuis Andre Vale-SilvaSarah PralletCharlotte C. DeckerRebecca M. FuBingqian QuKunihiro UryuHenrik MolinaMohsan SaeedEike SteinmannStephan UrbanRoshni R. SingarajaWilliam M. SchneiderSanford M. SimonCharles M. RiceNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-13 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Viet Loan Dao Thi Xianfang Wu Rachel L. Belote Ursula Andreo Constantin N. Takacs Joseph P. Fernandez Luis Andre Vale-Silva Sarah Prallet Charlotte C. Decker Rebecca M. Fu Bingqian Qu Kunihiro Uryu Henrik Molina Mohsan Saeed Eike Steinmann Stephan Urban Roshni R. Singaraja William M. Schneider Sanford M. Simon Charles M. Rice Stem cell-derived polarized hepatocytes
description	To model hepatocyte function accurately in vitro, it is necessary to generate and maintain a polarized epithelium. Here, the authors describe a protocol to generate polarized human pluripotent stem cell-derived hepatocyte-like cells (HLCs) to model enteric virus production and drug secretion in vitro.
format	article
author	Viet Loan Dao Thi Xianfang Wu Rachel L. Belote Ursula Andreo Constantin N. Takacs Joseph P. Fernandez Luis Andre Vale-Silva Sarah Prallet Charlotte C. Decker Rebecca M. Fu Bingqian Qu Kunihiro Uryu Henrik Molina Mohsan Saeed Eike Steinmann Stephan Urban Roshni R. Singaraja William M. Schneider Sanford M. Simon Charles M. Rice
author_facet	Viet Loan Dao Thi Xianfang Wu Rachel L. Belote Ursula Andreo Constantin N. Takacs Joseph P. Fernandez Luis Andre Vale-Silva Sarah Prallet Charlotte C. Decker Rebecca M. Fu Bingqian Qu Kunihiro Uryu Henrik Molina Mohsan Saeed Eike Steinmann Stephan Urban Roshni R. Singaraja William M. Schneider Sanford M. Simon Charles M. Rice
author_sort	Viet Loan Dao Thi
title	Stem cell-derived polarized hepatocytes
title_short	Stem cell-derived polarized hepatocytes
title_full	Stem cell-derived polarized hepatocytes
title_fullStr	Stem cell-derived polarized hepatocytes
title_full_unstemmed	Stem cell-derived polarized hepatocytes
title_sort	stem cell-derived polarized hepatocytes
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/d2bbfc7838fb493fa97035285b6d1783
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Stem cell-derived polarized hepatocytes

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