In vivo Raman spectral analysis of impaired cervical remodeling in a mouse model of delayed parturition

Abstract Monitoring cervical structure and composition during pregnancy has high potential for prediction of preterm birth (PTB), a problem affecting 15 million newborns annually. We use in vivo Raman spectroscopy, a label-free, light-based method that provides a molecular fingerprint to non-invasiv...

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Autores principales:	Christine M. O’Brien, Jennifer L. Herington, Naoko Brown, Isaac J. Pence, Bibhash C. Paria, James C. Slaughter, Jeff Reese, Anita Mahadevan-Jansen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d2ca99fcdf884331ba7dd18a534bec5d
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spelling	oai:doaj.org-article:d2ca99fcdf884331ba7dd18a534bec5d2021-12-02T11:52:27ZIn vivo Raman spectral analysis of impaired cervical remodeling in a mouse model of delayed parturition10.1038/s41598-017-07047-52045-2322https://doaj.org/article/d2ca99fcdf884331ba7dd18a534bec5d2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07047-5https://doaj.org/toc/2045-2322Abstract Monitoring cervical structure and composition during pregnancy has high potential for prediction of preterm birth (PTB), a problem affecting 15 million newborns annually. We use in vivo Raman spectroscopy, a label-free, light-based method that provides a molecular fingerprint to non-invasively investigate normal and impaired cervical remodeling. Prostaglandins stimulate uterine contractions and are clinically used for cervical ripening during pregnancy. Deletion of cyclooxygenase-1 (Cox-1), an enzyme involved in production of these prostaglandins, results in delayed parturition in mice. Contrary to expectation, Cox-1 null mice displayed normal uterine contractility; therefore, this study sought to determine whether cervical changes could explain the parturition differences in Cox-1 null mice and gestation-matched wild type (WT) controls. Raman spectral changes related to extracellular matrix proteins, lipids, and nucleic acids were tracked over pregnancy and found to be significantly delayed in Cox-1 null mice at term. A cervical basis for the parturition delay was confirmed by other ex vivo tests including decreased tissue distensibility, hydration, and elevated progesterone levels in the Cox-1 null mice at term. In conclusion, in vivo Raman spectroscopy non-invasively detected abnormal remodeling in the Cox-1 null mouse, and clearly demonstrated that the cervix plays a key role in their delayed parturition.Christine M. O’BrienJennifer L. HeringtonNaoko BrownIsaac J. PenceBibhash C. PariaJames C. SlaughterJeff ReeseAnita Mahadevan-JansenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-14 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Christine M. O’Brien Jennifer L. Herington Naoko Brown Isaac J. Pence Bibhash C. Paria James C. Slaughter Jeff Reese Anita Mahadevan-Jansen In vivo Raman spectral analysis of impaired cervical remodeling in a mouse model of delayed parturition
description	Abstract Monitoring cervical structure and composition during pregnancy has high potential for prediction of preterm birth (PTB), a problem affecting 15 million newborns annually. We use in vivo Raman spectroscopy, a label-free, light-based method that provides a molecular fingerprint to non-invasively investigate normal and impaired cervical remodeling. Prostaglandins stimulate uterine contractions and are clinically used for cervical ripening during pregnancy. Deletion of cyclooxygenase-1 (Cox-1), an enzyme involved in production of these prostaglandins, results in delayed parturition in mice. Contrary to expectation, Cox-1 null mice displayed normal uterine contractility; therefore, this study sought to determine whether cervical changes could explain the parturition differences in Cox-1 null mice and gestation-matched wild type (WT) controls. Raman spectral changes related to extracellular matrix proteins, lipids, and nucleic acids were tracked over pregnancy and found to be significantly delayed in Cox-1 null mice at term. A cervical basis for the parturition delay was confirmed by other ex vivo tests including decreased tissue distensibility, hydration, and elevated progesterone levels in the Cox-1 null mice at term. In conclusion, in vivo Raman spectroscopy non-invasively detected abnormal remodeling in the Cox-1 null mouse, and clearly demonstrated that the cervix plays a key role in their delayed parturition.
format	article
author	Christine M. O’Brien Jennifer L. Herington Naoko Brown Isaac J. Pence Bibhash C. Paria James C. Slaughter Jeff Reese Anita Mahadevan-Jansen
author_facet	Christine M. O’Brien Jennifer L. Herington Naoko Brown Isaac J. Pence Bibhash C. Paria James C. Slaughter Jeff Reese Anita Mahadevan-Jansen
author_sort	Christine M. O’Brien
title	In vivo Raman spectral analysis of impaired cervical remodeling in a mouse model of delayed parturition
title_short	In vivo Raman spectral analysis of impaired cervical remodeling in a mouse model of delayed parturition
title_full	In vivo Raman spectral analysis of impaired cervical remodeling in a mouse model of delayed parturition
title_fullStr	In vivo Raman spectral analysis of impaired cervical remodeling in a mouse model of delayed parturition
title_full_unstemmed	In vivo Raman spectral analysis of impaired cervical remodeling in a mouse model of delayed parturition
title_sort	in vivo raman spectral analysis of impaired cervical remodeling in a mouse model of delayed parturition
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/d2ca99fcdf884331ba7dd18a534bec5d
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In vivo Raman spectral analysis of impaired cervical remodeling in a mouse model of delayed parturition

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