Analysis of the role of Purα in the pathogenesis of Alzheimer's disease based on RNA-seq and ChIP-seq
Abstract Purine rich element binding protein A (Purα), encoded by the Purα gene, is an important transcriptional regulator that binds to DNA and RNA and is involved in processes such as DNA replication and RNA translation. Purα also plays an important role in the nervous system. To identify the func...
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2021
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oai:doaj.org-article:d2d187471144438c9ae14bd3f8a441e12021-12-02T17:30:53ZAnalysis of the role of Purα in the pathogenesis of Alzheimer's disease based on RNA-seq and ChIP-seq10.1038/s41598-021-90982-12045-2322https://doaj.org/article/d2d187471144438c9ae14bd3f8a441e12021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-90982-1https://doaj.org/toc/2045-2322Abstract Purine rich element binding protein A (Purα), encoded by the Purα gene, is an important transcriptional regulator that binds to DNA and RNA and is involved in processes such as DNA replication and RNA translation. Purα also plays an important role in the nervous system. To identify the function of Pura, we performed RNA sequence (RNA-seq) analysis of Purɑ-KO mouse hippocampal neuron cell line (HT22) to analyze the effect of Purα deletion on neuronal expression profiles. And combined with ChIP-seq analysis to explore the mechanism of Purα on gene regulation. In the end, totaly 656 differentially expressed genes between HT22 and Purα-KO HT22 cells have been found, which include 7 Alzheimer’s disease (AD)-related genes and 5 Aβ clearance related genes. 47 genes were regulated by Purα directly, the evidence based on CHIP-seq, which include Insr, Mapt, Vldlr, Jag1, etc. Our study provides the important informations of Purα in neuro-development. The possible regulative effects of Purα on AD-related genes consist inthe direct and indirect pathways of Purα in the pathogenesis of AD.Xiaoguang ShiShuanglai RenBingying ZhangShanshan GuoWenxin HeChengmin YuanXiaofan YangKevin Ig-lzevbekhaiTao SunQinwen WangJianqi CuiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021) |
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Medicine R Science Q Xiaoguang Shi Shuanglai Ren Bingying Zhang Shanshan Guo Wenxin He Chengmin Yuan Xiaofan Yang Kevin Ig-lzevbekhai Tao Sun Qinwen Wang Jianqi Cui Analysis of the role of Purα in the pathogenesis of Alzheimer's disease based on RNA-seq and ChIP-seq |
| description |
Abstract Purine rich element binding protein A (Purα), encoded by the Purα gene, is an important transcriptional regulator that binds to DNA and RNA and is involved in processes such as DNA replication and RNA translation. Purα also plays an important role in the nervous system. To identify the function of Pura, we performed RNA sequence (RNA-seq) analysis of Purɑ-KO mouse hippocampal neuron cell line (HT22) to analyze the effect of Purα deletion on neuronal expression profiles. And combined with ChIP-seq analysis to explore the mechanism of Purα on gene regulation. In the end, totaly 656 differentially expressed genes between HT22 and Purα-KO HT22 cells have been found, which include 7 Alzheimer’s disease (AD)-related genes and 5 Aβ clearance related genes. 47 genes were regulated by Purα directly, the evidence based on CHIP-seq, which include Insr, Mapt, Vldlr, Jag1, etc. Our study provides the important informations of Purα in neuro-development. The possible regulative effects of Purα on AD-related genes consist inthe direct and indirect pathways of Purα in the pathogenesis of AD. |
| format |
article |
| author |
Xiaoguang Shi Shuanglai Ren Bingying Zhang Shanshan Guo Wenxin He Chengmin Yuan Xiaofan Yang Kevin Ig-lzevbekhai Tao Sun Qinwen Wang Jianqi Cui |
| author_facet |
Xiaoguang Shi Shuanglai Ren Bingying Zhang Shanshan Guo Wenxin He Chengmin Yuan Xiaofan Yang Kevin Ig-lzevbekhai Tao Sun Qinwen Wang Jianqi Cui |
| author_sort |
Xiaoguang Shi |
| title |
Analysis of the role of Purα in the pathogenesis of Alzheimer's disease based on RNA-seq and ChIP-seq |
| title_short |
Analysis of the role of Purα in the pathogenesis of Alzheimer's disease based on RNA-seq and ChIP-seq |
| title_full |
Analysis of the role of Purα in the pathogenesis of Alzheimer's disease based on RNA-seq and ChIP-seq |
| title_fullStr |
Analysis of the role of Purα in the pathogenesis of Alzheimer's disease based on RNA-seq and ChIP-seq |
| title_full_unstemmed |
Analysis of the role of Purα in the pathogenesis of Alzheimer's disease based on RNA-seq and ChIP-seq |
| title_sort |
analysis of the role of purα in the pathogenesis of alzheimer's disease based on rna-seq and chip-seq |
| publisher |
Nature Portfolio |
| publishDate |
2021 |
| url |
https://doaj.org/article/d2d187471144438c9ae14bd3f8a441e1 |
| work_keys_str_mv |
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1718380757130412032 |