Mapping of dendritic lesions in patients with herpes simplex keratitis using in vivo confocal microscopy

Hideaki Yokogawa, Akira Kobayashi, Natsuko Mori, Kazuhisa Sugiyama Department of Ophthalmology, Kanazawa University Graduate School of Medical Science, Kanazawa, Japan Purpose: To produce a two-dimensional reconstruction map of dendritic lesions in patients with herpes simplex keratitis (HSK) usin...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Yokogawa H, Kobayashi A, Mori N, Sugiyama K
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2015
Materias:	Ophthalmology RE1-994
Acceso en línea:	https://doaj.org/article/d2d1cd553c3d4eb58de583256f8567e0
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:d2d1cd553c3d4eb58de583256f8567e0
record_format	dspace
spelling	oai:doaj.org-article:d2d1cd553c3d4eb58de583256f8567e02021-12-02T00:38:25ZMapping of dendritic lesions in patients with herpes simplex keratitis using in vivo confocal microscopy1177-5483https://doaj.org/article/d2d1cd553c3d4eb58de583256f8567e02015-09-01T00:00:00Zhttps://www.dovepress.com/mapping-of-dendritic-lesions-in-patients-with-herpes-simplex-keratitis-peer-reviewed-article-OPTHhttps://doaj.org/toc/1177-5483Hideaki Yokogawa, Akira Kobayashi, Natsuko Mori, Kazuhisa Sugiyama Department of Ophthalmology, Kanazawa University Graduate School of Medical Science, Kanazawa, Japan Purpose: To produce a two-dimensional reconstruction map of dendritic lesions in patients with herpes simplex keratitis (HSK) using in vivo confocal microscopy.Methods: Four eyes of four patients (mean 65.8 years) with HSK presenting with a dendritic lesion were enrolled. Slit-lamp biomicroscopy and in vivo laser confocal microscopy were performed. Acquired confocal images at the level of the epithelium were arranged and mapped into subconfluent montages. Changes in the shape and degree of light reflection of abnormal cells and deposits around dendritic lesions as well as other corneal layers were qualitatively evaluated.Results: Mapping of dendritic lesion was successful in all cases, and the subconfluent montages clearly showed the larger image of dendritic lesion. In all cases, the dendritic lesion consisted of hyperreflective irregular epithelial cells, and was surrounded by distorted and elongated epithelial cells. In three cases, hyperreflective deposits were noted at the midline of the lesion. The corneal stroma showed a hyperreflective honeycomb pattern. In two cases, inflammatory cells were observed at the level of endothelial cell layer.Conclusion: Mapping of dendritic lesions in patients with HSK was successful in all patients using in vivo confocal microscopy. Cellular level observation of dendritic lesion at a relatively larger magnification may help understand the in vivo morphological change of HSK. Further study in more patients with HSK and nonherpetic dendritic lesion is needed to utilize confocal microscopy images in differential diagnosis and follow-up of the epithelial lesions with dendrite. Keywords: herpetic epithelial keratitis, in vivo confocal microscopy, fluorescein slit-lamp photograph, hyperreflective irregular epithelial cells, dendritic lesionYokogawa HKobayashi AMori NSugiyama KDove Medical PressarticleOphthalmologyRE1-994ENClinical Ophthalmology, Vol 2015, Iss default, Pp 1771-1777 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	Ophthalmology RE1-994
spellingShingle	Ophthalmology RE1-994 Yokogawa H Kobayashi A Mori N Sugiyama K Mapping of dendritic lesions in patients with herpes simplex keratitis using in vivo confocal microscopy
description	Hideaki Yokogawa, Akira Kobayashi, Natsuko Mori, Kazuhisa Sugiyama Department of Ophthalmology, Kanazawa University Graduate School of Medical Science, Kanazawa, Japan Purpose: To produce a two-dimensional reconstruction map of dendritic lesions in patients with herpes simplex keratitis (HSK) using in vivo confocal microscopy.Methods: Four eyes of four patients (mean 65.8 years) with HSK presenting with a dendritic lesion were enrolled. Slit-lamp biomicroscopy and in vivo laser confocal microscopy were performed. Acquired confocal images at the level of the epithelium were arranged and mapped into subconfluent montages. Changes in the shape and degree of light reflection of abnormal cells and deposits around dendritic lesions as well as other corneal layers were qualitatively evaluated.Results: Mapping of dendritic lesion was successful in all cases, and the subconfluent montages clearly showed the larger image of dendritic lesion. In all cases, the dendritic lesion consisted of hyperreflective irregular epithelial cells, and was surrounded by distorted and elongated epithelial cells. In three cases, hyperreflective deposits were noted at the midline of the lesion. The corneal stroma showed a hyperreflective honeycomb pattern. In two cases, inflammatory cells were observed at the level of endothelial cell layer.Conclusion: Mapping of dendritic lesions in patients with HSK was successful in all patients using in vivo confocal microscopy. Cellular level observation of dendritic lesion at a relatively larger magnification may help understand the in vivo morphological change of HSK. Further study in more patients with HSK and nonherpetic dendritic lesion is needed to utilize confocal microscopy images in differential diagnosis and follow-up of the epithelial lesions with dendrite. Keywords: herpetic epithelial keratitis, in vivo confocal microscopy, fluorescein slit-lamp photograph, hyperreflective irregular epithelial cells, dendritic lesion
format	article
author	Yokogawa H Kobayashi A Mori N Sugiyama K
author_facet	Yokogawa H Kobayashi A Mori N Sugiyama K
author_sort	Yokogawa H
title	Mapping of dendritic lesions in patients with herpes simplex keratitis using in vivo confocal microscopy
title_short	Mapping of dendritic lesions in patients with herpes simplex keratitis using in vivo confocal microscopy
title_full	Mapping of dendritic lesions in patients with herpes simplex keratitis using in vivo confocal microscopy
title_fullStr	Mapping of dendritic lesions in patients with herpes simplex keratitis using in vivo confocal microscopy
title_full_unstemmed	Mapping of dendritic lesions in patients with herpes simplex keratitis using in vivo confocal microscopy
title_sort	mapping of dendritic lesions in patients with herpes simplex keratitis using in vivo confocal microscopy
publisher	Dove Medical Press
publishDate	2015
url	https://doaj.org/article/d2d1cd553c3d4eb58de583256f8567e0
work_keys_str_mv	AT yokogawah mappingofdendriticlesionsinpatientswithherpessimplexkeratitisusinginvivoconfocalmicroscopy AT kobayashia mappingofdendriticlesionsinpatientswithherpessimplexkeratitisusinginvivoconfocalmicroscopy AT morin mappingofdendriticlesionsinpatientswithherpessimplexkeratitisusinginvivoconfocalmicroscopy AT sugiyamak mappingofdendriticlesionsinpatientswithherpessimplexkeratitisusinginvivoconfocalmicroscopy
_version_	1718403584281804800

Mapping of dendritic lesions in patients with herpes simplex keratitis using in vivo confocal microscopy

Ejemplares similares