Bioactivity Screening of Endophytic Fungus Eutypa linearis isolated from Coleus amboinicus (Lour.)

Coleus amboinicus (Lour.) is a medicinal plant containing various bioactive compounds. Endophytes are microorganisms living inside intracellular tissue of plants and known as the source of bioactive compounds. In order to explore the potential of endophyte in producing novel bioactive compounds, thi...

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Autores principales:	Baiq Maylinda Gemantari, Fitra Romadhonsyah, Arief Nurrochmad, Subagus Wahyuono, Puji Astuti
Formato:	article
Lenguaje:	EN
Publicado:	Universitas Gadjah Mada 2021
Materias:	eutypa linearis, submerged fermentation, cytotoxicity, antioxidant, endophyte. Pharmacy and materia medica RS1-441
Acceso en línea:	https://doaj.org/article/d2d627ee7b1d4e968f97d6ac107daece
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spelling	oai:doaj.org-article:d2d627ee7b1d4e968f97d6ac107daece2021-11-15T06:08:47ZBioactivity Screening of Endophytic Fungus Eutypa linearis isolated from Coleus amboinicus (Lour.)2338-94272338-948610.22146/ijp.1077https://doaj.org/article/d2d627ee7b1d4e968f97d6ac107daece2021-03-01T00:00:00Zhttps://jurnal.ugm.ac.id/v3/IJP/article/view/1077https://doaj.org/toc/2338-9427https://doaj.org/toc/2338-9486Coleus amboinicus (Lour.) is a medicinal plant containing various bioactive compounds. Endophytes are microorganisms living inside intracellular tissue of plants and known as the source of bioactive compounds. In order to explore the potential of endophyte in producing novel bioactive compounds, this study focused on isolating endophytic fungi from the leaves of C.amboinicus, characterisation and screening their metabolite bioactivity during submerged culture fermentation. Isolation of endophytic fungi from the leaves segment of C.amboinicus was conducted on PDA media and fungus identification was carried out by analyzing its morphology and molecular examination. The production of metabolites was examined using thin layer chromatography and gas chromatography. Identification of the endophytic fungus showed 98.84% similarity with Eutypa linearis. This species was fermented submergedly in PDB medium for 14 days under dark and exposed to light and the fermentation broth was extracted using ethyl acetate. The results showed that exposure to the light did not significantly influence metabolite production. The ethyl acetate extract exhibited antioxidant and cytotoxic activities. Antioxidant activity of this extract as examined by DPPH assay showed IC50 of 105.31 ± 2.11 µg/mL. Cytotoxic activity against Hela cell line was known to be the best among other cell lines (IC50 301.53 ± 11.34 µg/mL) although it was found to be non selective (SI<1). The extract contained five major compounds namely Benzenemethanol, 4-nitro-(CAS) p-Nitrobenzyl alcohol; 2-Pentadecanone (CAS) Pentadecan-2-one; (1R,6S,10R*)-5,5-Dimethyl-11,12-dioxatricyclo[8.2.1.0(1,6)] tridecan-10-ol; 9,12-Octadecadienoic acid (Z,Z)-, methyl ester (CAS) Methyl linoleate; and 3-Furanacetic acid, 4-hexyl-2,5-dihydro-2,5-dioxo- (CAS) 2-carboxymethyl-3-N-hexyl-maleic anhydride.Baiq Maylinda GemantariFitra RomadhonsyahArief NurrochmadSubagus WahyuonoPuji AstutiUniversitas Gadjah Madaarticleeutypa linearis, submerged fermentation, cytotoxicity, antioxidant, endophyte.Pharmacy and materia medicaRS1-441ENIndonesian Journal of Pharmacy, Pp 86-95 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	eutypa linearis, submerged fermentation, cytotoxicity, antioxidant, endophyte. Pharmacy and materia medica RS1-441
spellingShingle	eutypa linearis, submerged fermentation, cytotoxicity, antioxidant, endophyte. Pharmacy and materia medica RS1-441 Baiq Maylinda Gemantari Fitra Romadhonsyah Arief Nurrochmad Subagus Wahyuono Puji Astuti Bioactivity Screening of Endophytic Fungus Eutypa linearis isolated from Coleus amboinicus (Lour.)
description	Coleus amboinicus (Lour.) is a medicinal plant containing various bioactive compounds. Endophytes are microorganisms living inside intracellular tissue of plants and known as the source of bioactive compounds. In order to explore the potential of endophyte in producing novel bioactive compounds, this study focused on isolating endophytic fungi from the leaves of C.amboinicus, characterisation and screening their metabolite bioactivity during submerged culture fermentation. Isolation of endophytic fungi from the leaves segment of C.amboinicus was conducted on PDA media and fungus identification was carried out by analyzing its morphology and molecular examination. The production of metabolites was examined using thin layer chromatography and gas chromatography. Identification of the endophytic fungus showed 98.84% similarity with Eutypa linearis. This species was fermented submergedly in PDB medium for 14 days under dark and exposed to light and the fermentation broth was extracted using ethyl acetate. The results showed that exposure to the light did not significantly influence metabolite production. The ethyl acetate extract exhibited antioxidant and cytotoxic activities. Antioxidant activity of this extract as examined by DPPH assay showed IC50 of 105.31 ± 2.11 µg/mL. Cytotoxic activity against Hela cell line was known to be the best among other cell lines (IC50 301.53 ± 11.34 µg/mL) although it was found to be non selective (SI<1). The extract contained five major compounds namely Benzenemethanol, 4-nitro-(CAS) p-Nitrobenzyl alcohol; 2-Pentadecanone (CAS) Pentadecan-2-one; (1R,6S,10R*)-5,5-Dimethyl-11,12-dioxatricyclo[8.2.1.0(1,6)] tridecan-10-ol; 9,12-Octadecadienoic acid (Z,Z)-, methyl ester (CAS) Methyl linoleate; and 3-Furanacetic acid, 4-hexyl-2,5-dihydro-2,5-dioxo- (CAS) 2-carboxymethyl-3-N-hexyl-maleic anhydride.
format	article
author	Baiq Maylinda Gemantari Fitra Romadhonsyah Arief Nurrochmad Subagus Wahyuono Puji Astuti
author_facet	Baiq Maylinda Gemantari Fitra Romadhonsyah Arief Nurrochmad Subagus Wahyuono Puji Astuti
author_sort	Baiq Maylinda Gemantari
title	Bioactivity Screening of Endophytic Fungus Eutypa linearis isolated from Coleus amboinicus (Lour.)
title_short	Bioactivity Screening of Endophytic Fungus Eutypa linearis isolated from Coleus amboinicus (Lour.)
title_full	Bioactivity Screening of Endophytic Fungus Eutypa linearis isolated from Coleus amboinicus (Lour.)
title_fullStr	Bioactivity Screening of Endophytic Fungus Eutypa linearis isolated from Coleus amboinicus (Lour.)
title_full_unstemmed	Bioactivity Screening of Endophytic Fungus Eutypa linearis isolated from Coleus amboinicus (Lour.)
title_sort	bioactivity screening of endophytic fungus eutypa linearis isolated from coleus amboinicus (lour.)
publisher	Universitas Gadjah Mada
publishDate	2021
url	https://doaj.org/article/d2d627ee7b1d4e968f97d6ac107daece
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Bioactivity Screening of Endophytic Fungus Eutypa linearis isolated from Coleus amboinicus (Lour.)

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