Hippocampal inactivation with TTX impairs long-term spatial memory retrieval and modifies brain metabolic activity.

Functional inactivation techniques enable studying the hippocampal involvement in each phase of spatial memory formation in the rat. In this study, we applied tetrodotoxin unilaterally or bilaterally into the dorsal hippocampus to evaluate the role of this brain structure in retrieval of memories ac...

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Autores principales:	Nélida María Conejo, José Manuel Cimadevilla, Héctor González-Pardo, Marta Méndez-Couz, Jorge Luis Arias
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d2db8b80f4eb4d5991e6ed0f8ca303e6
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spelling	oai:doaj.org-article:d2db8b80f4eb4d5991e6ed0f8ca303e62021-11-18T07:44:04ZHippocampal inactivation with TTX impairs long-term spatial memory retrieval and modifies brain metabolic activity.1932-620310.1371/journal.pone.0064749https://doaj.org/article/d2db8b80f4eb4d5991e6ed0f8ca303e62013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23724089/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Functional inactivation techniques enable studying the hippocampal involvement in each phase of spatial memory formation in the rat. In this study, we applied tetrodotoxin unilaterally or bilaterally into the dorsal hippocampus to evaluate the role of this brain structure in retrieval of memories acquired 28 days before in the Morris water maze. We combined hippocampal inactivation with the assessment of brain metabolism using cytochrome oxidase histochemistry. Several brain regions were considered, including the hippocampus and other related structures. Results showed that both unilateral and bilateral hippocampal inactivation impaired spatial memory retrieval. Hence, whereas subjects with bilateral hippocampal inactivation showed a circular swim pattern at the side walls of the pool, unilateral inactivation favoured swimming in the quadrants adjacent to the target one. Analysis of cytochrome oxidase activity disclosed regional differences according to the degree of hippocampal functional blockade. In comparison to control group, animals with bilateral inactivation showed increased CO activity in CA1 and CA3 areas of the hippocampus during retrieval, while the activity of the dentate gyrus substantially decreased. However, unilateral inactivated animals showed decreased CO activity in Ammon's horn and the dentate gyrus. This study demonstrated that retrieval recruits differentially the hippocampal subregions and the balance between them is altered with hippocampal functional lesions.Nélida María ConejoJosé Manuel CimadevillaHéctor González-PardoMarta Méndez-CouzJorge Luis AriasPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 5, p e64749 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Nélida María Conejo José Manuel Cimadevilla Héctor González-Pardo Marta Méndez-Couz Jorge Luis Arias Hippocampal inactivation with TTX impairs long-term spatial memory retrieval and modifies brain metabolic activity.
description	Functional inactivation techniques enable studying the hippocampal involvement in each phase of spatial memory formation in the rat. In this study, we applied tetrodotoxin unilaterally or bilaterally into the dorsal hippocampus to evaluate the role of this brain structure in retrieval of memories acquired 28 days before in the Morris water maze. We combined hippocampal inactivation with the assessment of brain metabolism using cytochrome oxidase histochemistry. Several brain regions were considered, including the hippocampus and other related structures. Results showed that both unilateral and bilateral hippocampal inactivation impaired spatial memory retrieval. Hence, whereas subjects with bilateral hippocampal inactivation showed a circular swim pattern at the side walls of the pool, unilateral inactivation favoured swimming in the quadrants adjacent to the target one. Analysis of cytochrome oxidase activity disclosed regional differences according to the degree of hippocampal functional blockade. In comparison to control group, animals with bilateral inactivation showed increased CO activity in CA1 and CA3 areas of the hippocampus during retrieval, while the activity of the dentate gyrus substantially decreased. However, unilateral inactivated animals showed decreased CO activity in Ammon's horn and the dentate gyrus. This study demonstrated that retrieval recruits differentially the hippocampal subregions and the balance between them is altered with hippocampal functional lesions.
format	article
author	Nélida María Conejo José Manuel Cimadevilla Héctor González-Pardo Marta Méndez-Couz Jorge Luis Arias
author_facet	Nélida María Conejo José Manuel Cimadevilla Héctor González-Pardo Marta Méndez-Couz Jorge Luis Arias
author_sort	Nélida María Conejo
title	Hippocampal inactivation with TTX impairs long-term spatial memory retrieval and modifies brain metabolic activity.
title_short	Hippocampal inactivation with TTX impairs long-term spatial memory retrieval and modifies brain metabolic activity.
title_full	Hippocampal inactivation with TTX impairs long-term spatial memory retrieval and modifies brain metabolic activity.
title_fullStr	Hippocampal inactivation with TTX impairs long-term spatial memory retrieval and modifies brain metabolic activity.
title_full_unstemmed	Hippocampal inactivation with TTX impairs long-term spatial memory retrieval and modifies brain metabolic activity.
title_sort	hippocampal inactivation with ttx impairs long-term spatial memory retrieval and modifies brain metabolic activity.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/d2db8b80f4eb4d5991e6ed0f8ca303e6
work_keys_str_mv	AT nelidamariaconejo hippocampalinactivationwithttximpairslongtermspatialmemoryretrievalandmodifiesbrainmetabolicactivity AT josemanuelcimadevilla hippocampalinactivationwithttximpairslongtermspatialmemoryretrievalandmodifiesbrainmetabolicactivity AT hectorgonzalezpardo hippocampalinactivationwithttximpairslongtermspatialmemoryretrievalandmodifiesbrainmetabolicactivity AT martamendezcouz hippocampalinactivationwithttximpairslongtermspatialmemoryretrievalandmodifiesbrainmetabolicactivity AT jorgeluisarias hippocampalinactivationwithttximpairslongtermspatialmemoryretrievalandmodifiesbrainmetabolicactivity
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Hippocampal inactivation with TTX impairs long-term spatial memory retrieval and modifies brain metabolic activity.

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