Engineer chimeric Cas9 to expand PAM recognition based on evolutionary information

The genomic locations that can be targeted for editing by CRISPR are limited by the presence of the nuclease-specific PAM sequence. Here, the authors show PAM recognition can be expanded by replacing the key region in the PAM interaction domain of SaCas9 with the corresponding region of SaCas9 ortho...

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Autores principales:	Dacheng Ma, Zhimeng Xu, Zhaoyu Zhang, Xi Chen, Xiangzhi Zeng, Yiyang Zhang, Tingyue Deng, Mengfei Ren, Zheng Sun, Rui Jiang, Zhen Xie
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/d2dba04bf729424ba3ba8546bebedf29
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spelling	oai:doaj.org-article:d2dba04bf729424ba3ba8546bebedf292021-12-02T17:33:10ZEngineer chimeric Cas9 to expand PAM recognition based on evolutionary information10.1038/s41467-019-08395-82041-1723https://doaj.org/article/d2dba04bf729424ba3ba8546bebedf292019-02-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-08395-8https://doaj.org/toc/2041-1723The genomic locations that can be targeted for editing by CRISPR are limited by the presence of the nuclease-specific PAM sequence. Here, the authors show PAM recognition can be expanded by replacing the key region in the PAM interaction domain of SaCas9 with the corresponding region of SaCas9 orthologs.Dacheng MaZhimeng XuZhaoyu ZhangXi ChenXiangzhi ZengYiyang ZhangTingyue DengMengfei RenZheng SunRui JiangZhen XieNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-9 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Dacheng Ma Zhimeng Xu Zhaoyu Zhang Xi Chen Xiangzhi Zeng Yiyang Zhang Tingyue Deng Mengfei Ren Zheng Sun Rui Jiang Zhen Xie Engineer chimeric Cas9 to expand PAM recognition based on evolutionary information
description	The genomic locations that can be targeted for editing by CRISPR are limited by the presence of the nuclease-specific PAM sequence. Here, the authors show PAM recognition can be expanded by replacing the key region in the PAM interaction domain of SaCas9 with the corresponding region of SaCas9 orthologs.
format	article
author	Dacheng Ma Zhimeng Xu Zhaoyu Zhang Xi Chen Xiangzhi Zeng Yiyang Zhang Tingyue Deng Mengfei Ren Zheng Sun Rui Jiang Zhen Xie
author_facet	Dacheng Ma Zhimeng Xu Zhaoyu Zhang Xi Chen Xiangzhi Zeng Yiyang Zhang Tingyue Deng Mengfei Ren Zheng Sun Rui Jiang Zhen Xie
author_sort	Dacheng Ma
title	Engineer chimeric Cas9 to expand PAM recognition based on evolutionary information
title_short	Engineer chimeric Cas9 to expand PAM recognition based on evolutionary information
title_full	Engineer chimeric Cas9 to expand PAM recognition based on evolutionary information
title_fullStr	Engineer chimeric Cas9 to expand PAM recognition based on evolutionary information
title_full_unstemmed	Engineer chimeric Cas9 to expand PAM recognition based on evolutionary information
title_sort	engineer chimeric cas9 to expand pam recognition based on evolutionary information
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/d2dba04bf729424ba3ba8546bebedf29
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_version_	1718380091103248384

Engineer chimeric Cas9 to expand PAM recognition based on evolutionary information

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