Engineer chimeric Cas9 to expand PAM recognition based on evolutionary information

The genomic locations that can be targeted for editing by CRISPR are limited by the presence of the nuclease-specific PAM sequence. Here, the authors show PAM recognition can be expanded by replacing the key region in the PAM interaction domain of SaCas9 with the corresponding region of SaCas9 ortho...

Description complète

Enregistré dans:

Détails bibliographiques
Auteurs principaux:	Dacheng Ma, Zhimeng Xu, Zhaoyu Zhang, Xi Chen, Xiangzhi Zeng, Yiyang Zhang, Tingyue Deng, Mengfei Ren, Zheng Sun, Rui Jiang, Zhen Xie
Format:	article
Langue:	EN
Publié:	Nature Portfolio 2019
Sujets:	Science Q
Accès en ligne:	https://doaj.org/article/d2dba04bf729424ba3ba8546bebedf29
Tags:	Ajouter un tag Pas de tags, Soyez le premier à ajouter un tag!

id	oai:doaj.org-article:d2dba04bf729424ba3ba8546bebedf29
record_format	dspace
spelling	oai:doaj.org-article:d2dba04bf729424ba3ba8546bebedf292021-12-02T17:33:10ZEngineer chimeric Cas9 to expand PAM recognition based on evolutionary information10.1038/s41467-019-08395-82041-1723https://doaj.org/article/d2dba04bf729424ba3ba8546bebedf292019-02-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-08395-8https://doaj.org/toc/2041-1723The genomic locations that can be targeted for editing by CRISPR are limited by the presence of the nuclease-specific PAM sequence. Here, the authors show PAM recognition can be expanded by replacing the key region in the PAM interaction domain of SaCas9 with the corresponding region of SaCas9 orthologs.Dacheng MaZhimeng XuZhaoyu ZhangXi ChenXiangzhi ZengYiyang ZhangTingyue DengMengfei RenZheng SunRui JiangZhen XieNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-9 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Dacheng Ma Zhimeng Xu Zhaoyu Zhang Xi Chen Xiangzhi Zeng Yiyang Zhang Tingyue Deng Mengfei Ren Zheng Sun Rui Jiang Zhen Xie Engineer chimeric Cas9 to expand PAM recognition based on evolutionary information
description	The genomic locations that can be targeted for editing by CRISPR are limited by the presence of the nuclease-specific PAM sequence. Here, the authors show PAM recognition can be expanded by replacing the key region in the PAM interaction domain of SaCas9 with the corresponding region of SaCas9 orthologs.
format	article
author	Dacheng Ma Zhimeng Xu Zhaoyu Zhang Xi Chen Xiangzhi Zeng Yiyang Zhang Tingyue Deng Mengfei Ren Zheng Sun Rui Jiang Zhen Xie
author_facet	Dacheng Ma Zhimeng Xu Zhaoyu Zhang Xi Chen Xiangzhi Zeng Yiyang Zhang Tingyue Deng Mengfei Ren Zheng Sun Rui Jiang Zhen Xie
author_sort	Dacheng Ma
title	Engineer chimeric Cas9 to expand PAM recognition based on evolutionary information
title_short	Engineer chimeric Cas9 to expand PAM recognition based on evolutionary information
title_full	Engineer chimeric Cas9 to expand PAM recognition based on evolutionary information
title_fullStr	Engineer chimeric Cas9 to expand PAM recognition based on evolutionary information
title_full_unstemmed	Engineer chimeric Cas9 to expand PAM recognition based on evolutionary information
title_sort	engineer chimeric cas9 to expand pam recognition based on evolutionary information
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/d2dba04bf729424ba3ba8546bebedf29
work_keys_str_mv	AT dachengma engineerchimericcas9toexpandpamrecognitionbasedonevolutionaryinformation AT zhimengxu engineerchimericcas9toexpandpamrecognitionbasedonevolutionaryinformation AT zhaoyuzhang engineerchimericcas9toexpandpamrecognitionbasedonevolutionaryinformation AT xichen engineerchimericcas9toexpandpamrecognitionbasedonevolutionaryinformation AT xiangzhizeng engineerchimericcas9toexpandpamrecognitionbasedonevolutionaryinformation AT yiyangzhang engineerchimericcas9toexpandpamrecognitionbasedonevolutionaryinformation AT tingyuedeng engineerchimericcas9toexpandpamrecognitionbasedonevolutionaryinformation AT mengfeiren engineerchimericcas9toexpandpamrecognitionbasedonevolutionaryinformation AT zhengsun engineerchimericcas9toexpandpamrecognitionbasedonevolutionaryinformation AT ruijiang engineerchimericcas9toexpandpamrecognitionbasedonevolutionaryinformation AT zhenxie engineerchimericcas9toexpandpamrecognitionbasedonevolutionaryinformation
_version_	1718380091103248384

Engineer chimeric Cas9 to expand PAM recognition based on evolutionary information

Documents similaires