Impact of chromosomal inversions on the yeast DAL cluster.

Chromosomal rearrangements occur readily in nature and are a major reshaping force during genome evolution. Such large scale modifications are usually deleterious causing several fitness defects, but sometimes can confer an advantage and become adaptive. For example the DAL metabolic cluster in yeas...

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Autores principales:	Samina Naseeb, Daniela Delneri
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d2e0df89975c4043a015afc238b395f7
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spelling	oai:doaj.org-article:d2e0df89975c4043a015afc238b395f72021-11-18T07:08:45ZImpact of chromosomal inversions on the yeast DAL cluster.1932-620310.1371/journal.pone.0042022https://doaj.org/article/d2e0df89975c4043a015afc238b395f72012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22916115/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Chromosomal rearrangements occur readily in nature and are a major reshaping force during genome evolution. Such large scale modifications are usually deleterious causing several fitness defects, but sometimes can confer an advantage and become adaptive. For example the DAL metabolic cluster in yeast was assembled in recent evolutionary times in the Hemiascomycetes lineage, through a set of rearrangements that brought together the genes involved in the allantoin degradation pathway. In eukaryotes, the existence of physical clustering of genes with related functions supports the notion that neighbouring ORFs tend to be co-expressed and that the order of genes along the chromosomes may have biological significance, rather than being random as previously believed. In this study, we investigate the phenotypic effect that inversions have on the DAL gene cluster, expressed during nitrogen starvation. In all Saccharomyces "sensu stricto" species the order of the DAL cluster is conserved, while in the "sensu lato" species Naumovia castellii, which grows significantly worse than S. cerevisiae on allantoin, the cluster includes two nested inversions encompassing three DAL genes. We constructed several inverted and non-inverted S. cerevisiae strains possessing different inversions including those to mimic the configuration of the N. castellii DAL cluster. We showed that the inversion of DAL2 lower its own expression and reduces yeast fitness during nitrogen starvation. This rearrangement also altered the expression of the neighbouring genes DAL1 and DAL4. Moreover, we showed that the expression of the DAL4 anti-sense transcript (SUT614) does not change upon inversions of DAL2 and therefore is unlikely to be involved in its regulation. These results show that the order of the DAL cluster has an impact on the phenotype and gene expression, suggesting that these rearrangements may have been adaptive in the "sensu stricto" group in relation to the low availability of nitrogen in the environment.Samina NaseebDaniela DelneriPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 8, p e42022 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Samina Naseeb Daniela Delneri Impact of chromosomal inversions on the yeast DAL cluster.
description	Chromosomal rearrangements occur readily in nature and are a major reshaping force during genome evolution. Such large scale modifications are usually deleterious causing several fitness defects, but sometimes can confer an advantage and become adaptive. For example the DAL metabolic cluster in yeast was assembled in recent evolutionary times in the Hemiascomycetes lineage, through a set of rearrangements that brought together the genes involved in the allantoin degradation pathway. In eukaryotes, the existence of physical clustering of genes with related functions supports the notion that neighbouring ORFs tend to be co-expressed and that the order of genes along the chromosomes may have biological significance, rather than being random as previously believed. In this study, we investigate the phenotypic effect that inversions have on the DAL gene cluster, expressed during nitrogen starvation. In all Saccharomyces "sensu stricto" species the order of the DAL cluster is conserved, while in the "sensu lato" species Naumovia castellii, which grows significantly worse than S. cerevisiae on allantoin, the cluster includes two nested inversions encompassing three DAL genes. We constructed several inverted and non-inverted S. cerevisiae strains possessing different inversions including those to mimic the configuration of the N. castellii DAL cluster. We showed that the inversion of DAL2 lower its own expression and reduces yeast fitness during nitrogen starvation. This rearrangement also altered the expression of the neighbouring genes DAL1 and DAL4. Moreover, we showed that the expression of the DAL4 anti-sense transcript (SUT614) does not change upon inversions of DAL2 and therefore is unlikely to be involved in its regulation. These results show that the order of the DAL cluster has an impact on the phenotype and gene expression, suggesting that these rearrangements may have been adaptive in the "sensu stricto" group in relation to the low availability of nitrogen in the environment.
format	article
author	Samina Naseeb Daniela Delneri
author_facet	Samina Naseeb Daniela Delneri
author_sort	Samina Naseeb
title	Impact of chromosomal inversions on the yeast DAL cluster.
title_short	Impact of chromosomal inversions on the yeast DAL cluster.
title_full	Impact of chromosomal inversions on the yeast DAL cluster.
title_fullStr	Impact of chromosomal inversions on the yeast DAL cluster.
title_full_unstemmed	Impact of chromosomal inversions on the yeast DAL cluster.
title_sort	impact of chromosomal inversions on the yeast dal cluster.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/d2e0df89975c4043a015afc238b395f7
work_keys_str_mv	AT saminanaseeb impactofchromosomalinversionsontheyeastdalcluster AT danieladelneri impactofchromosomalinversionsontheyeastdalcluster
_version_	1718423883895275520

Impact of chromosomal inversions on the yeast DAL cluster.

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