SHOOT GROWTH1 maintains Arabidopsis epigenomes by regulating IBM1.

Maintaining correct DNA and histone methylation patterns is essential for the development of all eukaryotes. In Arabidopsis, we identified SHOOT GROWTH1 (SG1), a novel protein involved in the control of gene methylation. SG1 contains both a Bromo-Adjacent Homology (BAH) domain found in several chrom...

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Autores principales:	Vincent Coustham, Daniela Vlad, Aurélie Deremetz, Isabelle Gy, Francisco A Cubillos, Envel Kerdaffrec, Olivier Loudet, Nicolas Bouché
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d2e0e905b76d4c52b7bbb969e8623377
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spelling	oai:doaj.org-article:d2e0e905b76d4c52b7bbb969e86233772021-11-18T08:38:56ZSHOOT GROWTH1 maintains Arabidopsis epigenomes by regulating IBM1.1932-620310.1371/journal.pone.0084687https://doaj.org/article/d2e0e905b76d4c52b7bbb969e86233772014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24404182/?tool=EBIhttps://doaj.org/toc/1932-6203Maintaining correct DNA and histone methylation patterns is essential for the development of all eukaryotes. In Arabidopsis, we identified SHOOT GROWTH1 (SG1), a novel protein involved in the control of gene methylation. SG1 contains both a Bromo-Adjacent Homology (BAH) domain found in several chromatin regulators and an RNA-Recognition Motif (RRM). The sg1 mutations are associated with drastic pleiotropic phenotypes. The mutants degenerate after few generations and are similar to mutants of the histone demethylase INCREASE IN BONSAI METHYLATION1 (IBM1). A methylome analysis of sg1 mutants revealed a large number of gene bodies hypermethylated in the cytosine CHG context, associated with an increase in di-methylation of lysine 9 on histone H3 tail (H3K9me2), an epigenetic mark normally found in silenced transposons. The sg1 phenotype is suppressed by mutations in genes encoding the DNA methyltransferase CHROMOMETHYLASE3 (CMT3) or the histone methyltransferase KRYPTONITE (KYP), indicating that SG1 functions antagonistically to CMT3 or KYP. We further show that the IBM1 transcript is not correctly processed in sg1, and that the functional IBM1 transcript complements sg1. Altogether, our results suggest a function for SG1 in the maintenance of genome integrity by regulating IBM1.Vincent CousthamDaniela VladAurélie DeremetzIsabelle GyFrancisco A CubillosEnvel KerdaffrecOlivier LoudetNicolas BouchéPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 1, p e84687 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Vincent Coustham Daniela Vlad Aurélie Deremetz Isabelle Gy Francisco A Cubillos Envel Kerdaffrec Olivier Loudet Nicolas Bouché SHOOT GROWTH1 maintains Arabidopsis epigenomes by regulating IBM1.
description	Maintaining correct DNA and histone methylation patterns is essential for the development of all eukaryotes. In Arabidopsis, we identified SHOOT GROWTH1 (SG1), a novel protein involved in the control of gene methylation. SG1 contains both a Bromo-Adjacent Homology (BAH) domain found in several chromatin regulators and an RNA-Recognition Motif (RRM). The sg1 mutations are associated with drastic pleiotropic phenotypes. The mutants degenerate after few generations and are similar to mutants of the histone demethylase INCREASE IN BONSAI METHYLATION1 (IBM1). A methylome analysis of sg1 mutants revealed a large number of gene bodies hypermethylated in the cytosine CHG context, associated with an increase in di-methylation of lysine 9 on histone H3 tail (H3K9me2), an epigenetic mark normally found in silenced transposons. The sg1 phenotype is suppressed by mutations in genes encoding the DNA methyltransferase CHROMOMETHYLASE3 (CMT3) or the histone methyltransferase KRYPTONITE (KYP), indicating that SG1 functions antagonistically to CMT3 or KYP. We further show that the IBM1 transcript is not correctly processed in sg1, and that the functional IBM1 transcript complements sg1. Altogether, our results suggest a function for SG1 in the maintenance of genome integrity by regulating IBM1.
format	article
author	Vincent Coustham Daniela Vlad Aurélie Deremetz Isabelle Gy Francisco A Cubillos Envel Kerdaffrec Olivier Loudet Nicolas Bouché
author_facet	Vincent Coustham Daniela Vlad Aurélie Deremetz Isabelle Gy Francisco A Cubillos Envel Kerdaffrec Olivier Loudet Nicolas Bouché
author_sort	Vincent Coustham
title	SHOOT GROWTH1 maintains Arabidopsis epigenomes by regulating IBM1.
title_short	SHOOT GROWTH1 maintains Arabidopsis epigenomes by regulating IBM1.
title_full	SHOOT GROWTH1 maintains Arabidopsis epigenomes by regulating IBM1.
title_fullStr	SHOOT GROWTH1 maintains Arabidopsis epigenomes by regulating IBM1.
title_full_unstemmed	SHOOT GROWTH1 maintains Arabidopsis epigenomes by regulating IBM1.
title_sort	shoot growth1 maintains arabidopsis epigenomes by regulating ibm1.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/d2e0e905b76d4c52b7bbb969e8623377
work_keys_str_mv	AT vincentcoustham shootgrowth1maintainsarabidopsisepigenomesbyregulatingibm1 AT danielavlad shootgrowth1maintainsarabidopsisepigenomesbyregulatingibm1 AT aureliederemetz shootgrowth1maintainsarabidopsisepigenomesbyregulatingibm1 AT isabellegy shootgrowth1maintainsarabidopsisepigenomesbyregulatingibm1 AT franciscoacubillos shootgrowth1maintainsarabidopsisepigenomesbyregulatingibm1 AT envelkerdaffrec shootgrowth1maintainsarabidopsisepigenomesbyregulatingibm1 AT olivierloudet shootgrowth1maintainsarabidopsisepigenomesbyregulatingibm1 AT nicolasbouche shootgrowth1maintainsarabidopsisepigenomesbyregulatingibm1
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SHOOT GROWTH1 maintains Arabidopsis epigenomes by regulating IBM1.

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