Combination therapy with low-frequency ultrasound irradiation and radiofrequency ablation as a synergistic treatment for pancreatic cancer

We aim to evaluate the efficacies of combination therapy with low-frequency ultrasound-stimulated microbubbles (USMB) and radiofrequency ablation (RFA) on suppressing the proliferation of pancreatic cancer cell and treating Panc02 subcutaneous xenograft mice. The proliferation of HPDE6-C7 and Panc02...

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Autores principales:	Huiyang Wang, Wenxiu Ding, Hongwei Shi, Haiwei Bao, Yuting Lu, Tian an Jiang
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	low-frequency ultrasound-stimulated microbubbles radiofrequency ablation combined treatment panc02 cell apoptosis autophagy Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/d2e3617b830d4423b250c65fa8e7bac5
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spelling	oai:doaj.org-article:d2e3617b830d4423b250c65fa8e7bac52021-11-04T15:51:54ZCombination therapy with low-frequency ultrasound irradiation and radiofrequency ablation as a synergistic treatment for pancreatic cancer2165-59792165-598710.1080/21655979.2021.1995581https://doaj.org/article/d2e3617b830d4423b250c65fa8e7bac52021-10-01T00:00:00Zhttp://dx.doi.org/10.1080/21655979.2021.1995581https://doaj.org/toc/2165-5979https://doaj.org/toc/2165-5987We aim to evaluate the efficacies of combination therapy with low-frequency ultrasound-stimulated microbubbles (USMB) and radiofrequency ablation (RFA) on suppressing the proliferation of pancreatic cancer cell and treating Panc02 subcutaneous xenograft mice. The proliferation of HPDE6-C7 and Panc02 cells after the treatment of USMB and RFA alone or combination were evaluated by CCK-8 assay. Scratch test was performed to assess the cell migration capability. Panc02-bearing mice were received 14-day treatment of USMB and RFA alone or combination. Tumor size and survival rate was recorded once two days. The serum levels of immune-related factors and changes of apoptosis- and autophagy-related factors were detected by ELISA and western blotting methods. As a results, CKK-8 assays revealed significant inhibition on Panc02 cell proliferation in combination therapy with USMB and RFA relative to other groups (all p < 0.05). Strong synergistic effect of USMB combined with RFA was confirmed via the calculated combination index (CI) <0.4. In addition, combination therapy of USMB and RFA significantly inhibited the migration of Panc02 cells. Moreover, combined treatment remarkably inhibited the size and width of xenograft and improved the survival in Panc02-bearing mice. Furthermore, 14-day combination therapy of USMB and RFA in Panc02-bearing mice significantly facilitated the apoptosis and autophagy of tumor cells. In summary, combination therapy of USMB and RFA showed synergistic anti-tumor efficacies on Panc02 cells attributing to the promotion on apoptosis and autophagy in Panc02 subcutaneous xenograft mice.Huiyang WangWenxiu DingHongwei ShiHaiwei BaoYuting LuTian an JiangTaylor & Francis Grouparticlelow-frequency ultrasound-stimulated microbubblesradiofrequency ablationcombined treatmentpanc02 cellapoptosisautophagyBiotechnologyTP248.13-248.65ENBioengineered, Vol 0, Iss 0 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	low-frequency ultrasound-stimulated microbubbles radiofrequency ablation combined treatment panc02 cell apoptosis autophagy Biotechnology TP248.13-248.65
spellingShingle	low-frequency ultrasound-stimulated microbubbles radiofrequency ablation combined treatment panc02 cell apoptosis autophagy Biotechnology TP248.13-248.65 Huiyang Wang Wenxiu Ding Hongwei Shi Haiwei Bao Yuting Lu Tian an Jiang Combination therapy with low-frequency ultrasound irradiation and radiofrequency ablation as a synergistic treatment for pancreatic cancer
description	We aim to evaluate the efficacies of combination therapy with low-frequency ultrasound-stimulated microbubbles (USMB) and radiofrequency ablation (RFA) on suppressing the proliferation of pancreatic cancer cell and treating Panc02 subcutaneous xenograft mice. The proliferation of HPDE6-C7 and Panc02 cells after the treatment of USMB and RFA alone or combination were evaluated by CCK-8 assay. Scratch test was performed to assess the cell migration capability. Panc02-bearing mice were received 14-day treatment of USMB and RFA alone or combination. Tumor size and survival rate was recorded once two days. The serum levels of immune-related factors and changes of apoptosis- and autophagy-related factors were detected by ELISA and western blotting methods. As a results, CKK-8 assays revealed significant inhibition on Panc02 cell proliferation in combination therapy with USMB and RFA relative to other groups (all p < 0.05). Strong synergistic effect of USMB combined with RFA was confirmed via the calculated combination index (CI) <0.4. In addition, combination therapy of USMB and RFA significantly inhibited the migration of Panc02 cells. Moreover, combined treatment remarkably inhibited the size and width of xenograft and improved the survival in Panc02-bearing mice. Furthermore, 14-day combination therapy of USMB and RFA in Panc02-bearing mice significantly facilitated the apoptosis and autophagy of tumor cells. In summary, combination therapy of USMB and RFA showed synergistic anti-tumor efficacies on Panc02 cells attributing to the promotion on apoptosis and autophagy in Panc02 subcutaneous xenograft mice.
format	article
author	Huiyang Wang Wenxiu Ding Hongwei Shi Haiwei Bao Yuting Lu Tian an Jiang
author_facet	Huiyang Wang Wenxiu Ding Hongwei Shi Haiwei Bao Yuting Lu Tian an Jiang
author_sort	Huiyang Wang
title	Combination therapy with low-frequency ultrasound irradiation and radiofrequency ablation as a synergistic treatment for pancreatic cancer
title_short	Combination therapy with low-frequency ultrasound irradiation and radiofrequency ablation as a synergistic treatment for pancreatic cancer
title_full	Combination therapy with low-frequency ultrasound irradiation and radiofrequency ablation as a synergistic treatment for pancreatic cancer
title_fullStr	Combination therapy with low-frequency ultrasound irradiation and radiofrequency ablation as a synergistic treatment for pancreatic cancer
title_full_unstemmed	Combination therapy with low-frequency ultrasound irradiation and radiofrequency ablation as a synergistic treatment for pancreatic cancer
title_sort	combination therapy with low-frequency ultrasound irradiation and radiofrequency ablation as a synergistic treatment for pancreatic cancer
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/d2e3617b830d4423b250c65fa8e7bac5
work_keys_str_mv	AT huiyangwang combinationtherapywithlowfrequencyultrasoundirradiationandradiofrequencyablationasasynergistictreatmentforpancreaticcancer AT wenxiuding combinationtherapywithlowfrequencyultrasoundirradiationandradiofrequencyablationasasynergistictreatmentforpancreaticcancer AT hongweishi combinationtherapywithlowfrequencyultrasoundirradiationandradiofrequencyablationasasynergistictreatmentforpancreaticcancer AT haiweibao combinationtherapywithlowfrequencyultrasoundirradiationandradiofrequencyablationasasynergistictreatmentforpancreaticcancer AT yutinglu combinationtherapywithlowfrequencyultrasoundirradiationandradiofrequencyablationasasynergistictreatmentforpancreaticcancer AT tiananjiang combinationtherapywithlowfrequencyultrasoundirradiationandradiofrequencyablationasasynergistictreatmentforpancreaticcancer
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Combination therapy with low-frequency ultrasound irradiation and radiofrequency ablation as a synergistic treatment for pancreatic cancer

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