Novel LysM motifs for antigen display on lactobacilli for mucosal immunization

Abstract We characterized two LysM domains of Limosilactobacillus fermentum, belonging to proteins Acglu (GenBank: KPH22907.1) and Pgb (GenBank: KPH22047.1) and bacterium like particles (BLP) derived from the immunomodulatory strain Lacticaseibacillus rhamnosus IBL027 (BLPs027) as an antigen display...

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Autores principales:	Fernanda Raya-Tonetti, Melisa Müller, Jacinto Sacur, Haruki Kitazawa, Julio Villena, Maria Guadalupe Vizoso-Pinto
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d2eb770083574aacb0a410eea2ec33cb
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spelling	oai:doaj.org-article:d2eb770083574aacb0a410eea2ec33cb2021-11-08T10:50:34ZNovel LysM motifs for antigen display on lactobacilli for mucosal immunization10.1038/s41598-021-01087-82045-2322https://doaj.org/article/d2eb770083574aacb0a410eea2ec33cb2021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-01087-8https://doaj.org/toc/2045-2322Abstract We characterized two LysM domains of Limosilactobacillus fermentum, belonging to proteins Acglu (GenBank: KPH22907.1) and Pgb (GenBank: KPH22047.1) and bacterium like particles (BLP) derived from the immunomodulatory strain Lacticaseibacillus rhamnosus IBL027 (BLPs027) as an antigen display platform. The fluorescence protein Venus fused to the novel LysM domains could bind to the peptidoglycan shell of lactobacilli and resisted harsh conditions such as high NaCl and urea concentrations. Acglu with five LysM domains was a better anchor than Pgb baring only one domain. Six-week-old BALB/c mice were nasally immunized with the complex Venus-Acglu-BLPs027 at days 0, 14 and 28. The levels of specific serum IgG, IgG1 and IgG2a and the levels of total immunoglobulins (IgT) and IgA in broncho-alveolar lavage (BAL) were evaluated ten days after the last boosting. Venus-Acglu-BLPs027, nasally administered, significantly increased specific BAL IgT and IgA, and serum IgG levels. In addition, spleen cells of mice immunized with Venus-Acglu-BLPs027 secreted TNF-α, IFN-γ and IL-4 when stimulated ex vivo in a dose-dependent manner. We constructed a Gateway compatible destination vector to easily fuse the selected LysM domain to proteins of interest for antigen display to develop mucosal subunit vaccines.Fernanda Raya-TonettiMelisa MüllerJacinto SacurHaruki KitazawaJulio VillenaMaria Guadalupe Vizoso-PintoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Fernanda Raya-Tonetti Melisa Müller Jacinto Sacur Haruki Kitazawa Julio Villena Maria Guadalupe Vizoso-Pinto Novel LysM motifs for antigen display on lactobacilli for mucosal immunization
description	Abstract We characterized two LysM domains of Limosilactobacillus fermentum, belonging to proteins Acglu (GenBank: KPH22907.1) and Pgb (GenBank: KPH22047.1) and bacterium like particles (BLP) derived from the immunomodulatory strain Lacticaseibacillus rhamnosus IBL027 (BLPs027) as an antigen display platform. The fluorescence protein Venus fused to the novel LysM domains could bind to the peptidoglycan shell of lactobacilli and resisted harsh conditions such as high NaCl and urea concentrations. Acglu with five LysM domains was a better anchor than Pgb baring only one domain. Six-week-old BALB/c mice were nasally immunized with the complex Venus-Acglu-BLPs027 at days 0, 14 and 28. The levels of specific serum IgG, IgG1 and IgG2a and the levels of total immunoglobulins (IgT) and IgA in broncho-alveolar lavage (BAL) were evaluated ten days after the last boosting. Venus-Acglu-BLPs027, nasally administered, significantly increased specific BAL IgT and IgA, and serum IgG levels. In addition, spleen cells of mice immunized with Venus-Acglu-BLPs027 secreted TNF-α, IFN-γ and IL-4 when stimulated ex vivo in a dose-dependent manner. We constructed a Gateway compatible destination vector to easily fuse the selected LysM domain to proteins of interest for antigen display to develop mucosal subunit vaccines.
format	article
author	Fernanda Raya-Tonetti Melisa Müller Jacinto Sacur Haruki Kitazawa Julio Villena Maria Guadalupe Vizoso-Pinto
author_facet	Fernanda Raya-Tonetti Melisa Müller Jacinto Sacur Haruki Kitazawa Julio Villena Maria Guadalupe Vizoso-Pinto
author_sort	Fernanda Raya-Tonetti
title	Novel LysM motifs for antigen display on lactobacilli for mucosal immunization
title_short	Novel LysM motifs for antigen display on lactobacilli for mucosal immunization
title_full	Novel LysM motifs for antigen display on lactobacilli for mucosal immunization
title_fullStr	Novel LysM motifs for antigen display on lactobacilli for mucosal immunization
title_full_unstemmed	Novel LysM motifs for antigen display on lactobacilli for mucosal immunization
title_sort	novel lysm motifs for antigen display on lactobacilli for mucosal immunization
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/d2eb770083574aacb0a410eea2ec33cb
work_keys_str_mv	AT fernandarayatonetti novellysmmotifsforantigendisplayonlactobacilliformucosalimmunization AT melisamuller novellysmmotifsforantigendisplayonlactobacilliformucosalimmunization AT jacintosacur novellysmmotifsforantigendisplayonlactobacilliformucosalimmunization AT harukikitazawa novellysmmotifsforantigendisplayonlactobacilliformucosalimmunization AT juliovillena novellysmmotifsforantigendisplayonlactobacilliformucosalimmunization AT mariaguadalupevizosopinto novellysmmotifsforantigendisplayonlactobacilliformucosalimmunization
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Novel LysM motifs for antigen display on lactobacilli for mucosal immunization

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