A Cylindrical Molding Method for the Biofabrication of Plane-Shaped Skeletal Muscle Tissue

Muscle tissues can be fabricated in vitro by culturing myoblast-populated hydrogels. To counter the shrinkage of the myoblast-populated hydrogels during culture, a pair of anchors are generally utilized to fix the two ends of the hydrogel. Here, we propose an alternative method to counter the shrink...

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Autores principales:	Minghao Nie, Ai Shima, Kenta Fukushima, Yuya Morimoto, Shoji Takeuchi
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	biofabrication tissue engineering skeletal muscle microtissue assembly Mechanical engineering and machinery TJ1-1570
Acceso en línea:	https://doaj.org/article/d2ed996587a44f44a25479e629b686b0
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spelling	oai:doaj.org-article:d2ed996587a44f44a25479e629b686b02021-11-25T18:23:48ZA Cylindrical Molding Method for the Biofabrication of Plane-Shaped Skeletal Muscle Tissue10.3390/mi121114112072-666Xhttps://doaj.org/article/d2ed996587a44f44a25479e629b686b02021-11-01T00:00:00Zhttps://www.mdpi.com/2072-666X/12/11/1411https://doaj.org/toc/2072-666XMuscle tissues can be fabricated in vitro by culturing myoblast-populated hydrogels. To counter the shrinkage of the myoblast-populated hydrogels during culture, a pair of anchors are generally utilized to fix the two ends of the hydrogel. Here, we propose an alternative method to counter the shrinkage of the hydrogel and fabricate plane-shaped skeletal muscle tissues. The method forms myoblast-populated hydrogel in a cylindrical cavity with a central pillar, which can prevent tissue shrinkage along the circumferential direction. By eliminating the usages of the anchor pairs, our proposed method can produce plane-shaped skeletal muscle tissues with uniform width and thickness. In experiments, we demonstrate the fabrication of plane-shaped (length: ca. 10 mm, width: 5~15 mm) skeletal muscle tissue with submillimeter thickness. The tissues have uniform shapes and are populated with differentiated muscle cells stained positive for myogenic differentiation markers (i.e., myosin heavy chains). In addition, we show the assembly of subcentimeter-order tissue blocks by stacking the plane-shaped skeletal muscle tissues. The proposed method can be further optimized and scaled up to produce cultured animal products such as cultured meat.Minghao NieAi ShimaKenta FukushimaYuya MorimotoShoji TakeuchiMDPI AGarticlebiofabricationtissue engineeringskeletal musclemicrotissue assemblyMechanical engineering and machineryTJ1-1570ENMicromachines, Vol 12, Iss 1411, p 1411 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	biofabrication tissue engineering skeletal muscle microtissue assembly Mechanical engineering and machinery TJ1-1570
spellingShingle	biofabrication tissue engineering skeletal muscle microtissue assembly Mechanical engineering and machinery TJ1-1570 Minghao Nie Ai Shima Kenta Fukushima Yuya Morimoto Shoji Takeuchi A Cylindrical Molding Method for the Biofabrication of Plane-Shaped Skeletal Muscle Tissue
description	Muscle tissues can be fabricated in vitro by culturing myoblast-populated hydrogels. To counter the shrinkage of the myoblast-populated hydrogels during culture, a pair of anchors are generally utilized to fix the two ends of the hydrogel. Here, we propose an alternative method to counter the shrinkage of the hydrogel and fabricate plane-shaped skeletal muscle tissues. The method forms myoblast-populated hydrogel in a cylindrical cavity with a central pillar, which can prevent tissue shrinkage along the circumferential direction. By eliminating the usages of the anchor pairs, our proposed method can produce plane-shaped skeletal muscle tissues with uniform width and thickness. In experiments, we demonstrate the fabrication of plane-shaped (length: ca. 10 mm, width: 5~15 mm) skeletal muscle tissue with submillimeter thickness. The tissues have uniform shapes and are populated with differentiated muscle cells stained positive for myogenic differentiation markers (i.e., myosin heavy chains). In addition, we show the assembly of subcentimeter-order tissue blocks by stacking the plane-shaped skeletal muscle tissues. The proposed method can be further optimized and scaled up to produce cultured animal products such as cultured meat.
format	article
author	Minghao Nie Ai Shima Kenta Fukushima Yuya Morimoto Shoji Takeuchi
author_facet	Minghao Nie Ai Shima Kenta Fukushima Yuya Morimoto Shoji Takeuchi
author_sort	Minghao Nie
title	A Cylindrical Molding Method for the Biofabrication of Plane-Shaped Skeletal Muscle Tissue
title_short	A Cylindrical Molding Method for the Biofabrication of Plane-Shaped Skeletal Muscle Tissue
title_full	A Cylindrical Molding Method for the Biofabrication of Plane-Shaped Skeletal Muscle Tissue
title_fullStr	A Cylindrical Molding Method for the Biofabrication of Plane-Shaped Skeletal Muscle Tissue
title_full_unstemmed	A Cylindrical Molding Method for the Biofabrication of Plane-Shaped Skeletal Muscle Tissue
title_sort	cylindrical molding method for the biofabrication of plane-shaped skeletal muscle tissue
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/d2ed996587a44f44a25479e629b686b0
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A Cylindrical Molding Method for the Biofabrication of Plane-Shaped Skeletal Muscle Tissue

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