Stem cell therapy and curcumin synergistically enhance recovery from spinal cord injury.

Acute traumatic spinal cord injury (SCI) is marked by the enhanced production of local cytokines and pro-inflammatory substances that induce gliosis and prevent reinnervation. The transplantation of stem cells is a promising treatment strategy for SCI. In order to facilitate functional recovery, we...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	D Ryan Ormond, Craig Shannon, Julius Oppenheim, Richard Zeman, Kaushik Das, Raj Murali, Meena Jhanwar-Uniyal
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d2f5fb096b4d435ca8a150fe4006b9f4
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:d2f5fb096b4d435ca8a150fe4006b9f4
record_format	dspace
spelling	oai:doaj.org-article:d2f5fb096b4d435ca8a150fe4006b9f42021-11-18T08:32:13ZStem cell therapy and curcumin synergistically enhance recovery from spinal cord injury.1932-620310.1371/journal.pone.0088916https://doaj.org/article/d2f5fb096b4d435ca8a150fe4006b9f42014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24558450/?tool=EBIhttps://doaj.org/toc/1932-6203Acute traumatic spinal cord injury (SCI) is marked by the enhanced production of local cytokines and pro-inflammatory substances that induce gliosis and prevent reinnervation. The transplantation of stem cells is a promising treatment strategy for SCI. In order to facilitate functional recovery, we employed stem cell therapy alone or in combination with curcumin, a naturally-occurring anti-inflammatory component of turmeric (Curcuma longa), which potently inhibits NF-κB. Spinal cord contusion following laminectomy (T9-10) was performed using a weight drop apparatus (10 g over a 12.5 or 25 mm distance, representing moderate or severe SCI, respectively) in Sprague-Dawley rats. Neural stem cells (NSC) were isolated from subventricular zone (SVZ) and transplanted at the site of injury with or without curcumin treatment. Functional recovery was assessed by BBB score and body weight gain measured up to 6 weeks following SCI. At the conclusion of the study, the mass of soleus muscle was correlated with BBB score and body weight. Stem cell therapy improved recovery from moderate SCI, however, it had a limited effect on recovery after severe SCI. Curcumin stimulated NSC proliferation in vitro, and in combination with stem cell therapy, induced profound recovery from severe SCI as evidenced by improved functional locomotor recovery, increased body weight, and soleus muscle mass. These findings demonstrate that curcumin in conjunction with stem cell therapy synergistically improves recovery from severe SCI. Furthermore, our results indicate that the effect of curcumin extends beyond its known anti-inflammatory properties to the regulation of stem cell proliferation.D Ryan OrmondCraig ShannonJulius OppenheimRichard ZemanKaushik DasRaj MuraliMeena Jhanwar-UniyalPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 2, p e88916 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q D Ryan Ormond Craig Shannon Julius Oppenheim Richard Zeman Kaushik Das Raj Murali Meena Jhanwar-Uniyal Stem cell therapy and curcumin synergistically enhance recovery from spinal cord injury.
description	Acute traumatic spinal cord injury (SCI) is marked by the enhanced production of local cytokines and pro-inflammatory substances that induce gliosis and prevent reinnervation. The transplantation of stem cells is a promising treatment strategy for SCI. In order to facilitate functional recovery, we employed stem cell therapy alone or in combination with curcumin, a naturally-occurring anti-inflammatory component of turmeric (Curcuma longa), which potently inhibits NF-κB. Spinal cord contusion following laminectomy (T9-10) was performed using a weight drop apparatus (10 g over a 12.5 or 25 mm distance, representing moderate or severe SCI, respectively) in Sprague-Dawley rats. Neural stem cells (NSC) were isolated from subventricular zone (SVZ) and transplanted at the site of injury with or without curcumin treatment. Functional recovery was assessed by BBB score and body weight gain measured up to 6 weeks following SCI. At the conclusion of the study, the mass of soleus muscle was correlated with BBB score and body weight. Stem cell therapy improved recovery from moderate SCI, however, it had a limited effect on recovery after severe SCI. Curcumin stimulated NSC proliferation in vitro, and in combination with stem cell therapy, induced profound recovery from severe SCI as evidenced by improved functional locomotor recovery, increased body weight, and soleus muscle mass. These findings demonstrate that curcumin in conjunction with stem cell therapy synergistically improves recovery from severe SCI. Furthermore, our results indicate that the effect of curcumin extends beyond its known anti-inflammatory properties to the regulation of stem cell proliferation.
format	article
author	D Ryan Ormond Craig Shannon Julius Oppenheim Richard Zeman Kaushik Das Raj Murali Meena Jhanwar-Uniyal
author_facet	D Ryan Ormond Craig Shannon Julius Oppenheim Richard Zeman Kaushik Das Raj Murali Meena Jhanwar-Uniyal
author_sort	D Ryan Ormond
title	Stem cell therapy and curcumin synergistically enhance recovery from spinal cord injury.
title_short	Stem cell therapy and curcumin synergistically enhance recovery from spinal cord injury.
title_full	Stem cell therapy and curcumin synergistically enhance recovery from spinal cord injury.
title_fullStr	Stem cell therapy and curcumin synergistically enhance recovery from spinal cord injury.
title_full_unstemmed	Stem cell therapy and curcumin synergistically enhance recovery from spinal cord injury.
title_sort	stem cell therapy and curcumin synergistically enhance recovery from spinal cord injury.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/d2f5fb096b4d435ca8a150fe4006b9f4
work_keys_str_mv	AT dryanormond stemcelltherapyandcurcuminsynergisticallyenhancerecoveryfromspinalcordinjury AT craigshannon stemcelltherapyandcurcuminsynergisticallyenhancerecoveryfromspinalcordinjury AT juliusoppenheim stemcelltherapyandcurcuminsynergisticallyenhancerecoveryfromspinalcordinjury AT richardzeman stemcelltherapyandcurcuminsynergisticallyenhancerecoveryfromspinalcordinjury AT kaushikdas stemcelltherapyandcurcuminsynergisticallyenhancerecoveryfromspinalcordinjury AT rajmurali stemcelltherapyandcurcuminsynergisticallyenhancerecoveryfromspinalcordinjury AT meenajhanwaruniyal stemcelltherapyandcurcuminsynergisticallyenhancerecoveryfromspinalcordinjury
_version_	1718421711060205568

Stem cell therapy and curcumin synergistically enhance recovery from spinal cord injury.

Ejemplares similares