From intestinal permeability to dysmotility: the biobreeding rat as a model for functional gastrointestinal disorders.

<h4>Background</h4>Impaired intestinal barrier function, low-grade inflammation and altered neuronal control are reported in functional gastrointestinal disorders. However, the sequence of and causal relation between these events is unclear, necessitating a spontaneous animal model. The...

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Autores principales:	Tim Vanuytsel, Christophe Vanormelingen, Hanne Vanheel, Tatsuhiro Masaoka, Shadea Salim Rasoel, Joran Tóth, Els Houben, Kristin Verbeke, Gert De Hertogh, Pieter Vanden Berghe, Jan Tack, Ricard Farré
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d2fa767df3c64e2e9d3109e12721e31d
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spelling	oai:doaj.org-article:d2fa767df3c64e2e9d3109e12721e31d2021-11-25T05:55:06ZFrom intestinal permeability to dysmotility: the biobreeding rat as a model for functional gastrointestinal disorders.1932-620310.1371/journal.pone.0111132https://doaj.org/article/d2fa767df3c64e2e9d3109e12721e31d2014-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0111132https://doaj.org/toc/1932-6203<h4>Background</h4>Impaired intestinal barrier function, low-grade inflammation and altered neuronal control are reported in functional gastrointestinal disorders. However, the sequence of and causal relation between these events is unclear, necessitating a spontaneous animal model. The aim of this study was to describe the natural history of intestinal permeability, mucosal and neuromuscular inflammation and nitrergic motor neuron function during the lifetime of the BioBreeding (BB) rat.<h4>Methods</h4>Normoglycemic BB-diabetes prone (DP) and control rats were sacrificed at different ages and jejunum was harvested to characterize intestinal permeability, inflammation and neuromuscular function.<h4>Results</h4>Both structural and functional evidence of increased intestinal permeability was found in young BB-DP rats from the age of 50 days. In older animals, starting in the mucosa from 70 days and in half of the animals also in the muscularis propria from 110 days, an inflammatory reaction, characterized by an influx of polymorphonuclear cells and higher myeloperoxidase activity, was observed. Finally, in animals older than 110 days, coinciding with a myenteric ganglionitis, a loss of nitrergic neurons and motor function was demonstrated.<h4>Conclusion</h4>In the BB-rat, mucosal inflammatory cell infiltration is preceded by intestinal barrier dysfunction and followed by myenteric ganglionitis and loss of nitrergic function. This sequence supports a primary role for impaired barrier function and provides an insightful model for the pathogenesis of functional gastrointestinal disorders.Tim VanuytselChristophe VanormelingenHanne VanheelTatsuhiro MasaokaShadea Salim RasoelJoran TóthEls HoubenKristin VerbekeGert De HertoghPieter Vanden BergheJan TackRicard FarréPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 10, p e111132 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Tim Vanuytsel Christophe Vanormelingen Hanne Vanheel Tatsuhiro Masaoka Shadea Salim Rasoel Joran Tóth Els Houben Kristin Verbeke Gert De Hertogh Pieter Vanden Berghe Jan Tack Ricard Farré From intestinal permeability to dysmotility: the biobreeding rat as a model for functional gastrointestinal disorders.
description	<h4>Background</h4>Impaired intestinal barrier function, low-grade inflammation and altered neuronal control are reported in functional gastrointestinal disorders. However, the sequence of and causal relation between these events is unclear, necessitating a spontaneous animal model. The aim of this study was to describe the natural history of intestinal permeability, mucosal and neuromuscular inflammation and nitrergic motor neuron function during the lifetime of the BioBreeding (BB) rat.<h4>Methods</h4>Normoglycemic BB-diabetes prone (DP) and control rats were sacrificed at different ages and jejunum was harvested to characterize intestinal permeability, inflammation and neuromuscular function.<h4>Results</h4>Both structural and functional evidence of increased intestinal permeability was found in young BB-DP rats from the age of 50 days. In older animals, starting in the mucosa from 70 days and in half of the animals also in the muscularis propria from 110 days, an inflammatory reaction, characterized by an influx of polymorphonuclear cells and higher myeloperoxidase activity, was observed. Finally, in animals older than 110 days, coinciding with a myenteric ganglionitis, a loss of nitrergic neurons and motor function was demonstrated.<h4>Conclusion</h4>In the BB-rat, mucosal inflammatory cell infiltration is preceded by intestinal barrier dysfunction and followed by myenteric ganglionitis and loss of nitrergic function. This sequence supports a primary role for impaired barrier function and provides an insightful model for the pathogenesis of functional gastrointestinal disorders.
format	article
author	Tim Vanuytsel Christophe Vanormelingen Hanne Vanheel Tatsuhiro Masaoka Shadea Salim Rasoel Joran Tóth Els Houben Kristin Verbeke Gert De Hertogh Pieter Vanden Berghe Jan Tack Ricard Farré
author_facet	Tim Vanuytsel Christophe Vanormelingen Hanne Vanheel Tatsuhiro Masaoka Shadea Salim Rasoel Joran Tóth Els Houben Kristin Verbeke Gert De Hertogh Pieter Vanden Berghe Jan Tack Ricard Farré
author_sort	Tim Vanuytsel
title	From intestinal permeability to dysmotility: the biobreeding rat as a model for functional gastrointestinal disorders.
title_short	From intestinal permeability to dysmotility: the biobreeding rat as a model for functional gastrointestinal disorders.
title_full	From intestinal permeability to dysmotility: the biobreeding rat as a model for functional gastrointestinal disorders.
title_fullStr	From intestinal permeability to dysmotility: the biobreeding rat as a model for functional gastrointestinal disorders.
title_full_unstemmed	From intestinal permeability to dysmotility: the biobreeding rat as a model for functional gastrointestinal disorders.
title_sort	from intestinal permeability to dysmotility: the biobreeding rat as a model for functional gastrointestinal disorders.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/d2fa767df3c64e2e9d3109e12721e31d
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From intestinal permeability to dysmotility: the biobreeding rat as a model for functional gastrointestinal disorders.

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