Global Transcriptomic Analysis Reveals Differentially Expressed Genes Involved in Embryogenic Callus Induction in Drumstick (<i>Moringa oleifera</i> Lam.)

The plant embryogenic callus (EC) is an irregular embryogenic cell mass with strong regenerative ability that can be used for propagation and genetic transformation. However, difficulties with EC induction have hindered the breeding of drumstick, a tree with diverse potential commercial uses. In thi...

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Autores principales:	Endian Yang, Mingyang Zheng, Xuan Zou, Xiaoling Huang, Heyue Yang, Xiaoyang Chen, Junjie Zhang
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Moringa oleifera</i> embryogenic callus transcriptome differentially expressed genes Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/d300e2177e184322b00444c93be8d380
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spelling	oai:doaj.org-article:d300e2177e184322b00444c93be8d3802021-11-25T17:53:38ZGlobal Transcriptomic Analysis Reveals Differentially Expressed Genes Involved in Embryogenic Callus Induction in Drumstick (<i>Moringa oleifera</i> Lam.)10.3390/ijms2222121301422-00671661-6596https://doaj.org/article/d300e2177e184322b00444c93be8d3802021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/22/12130https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067The plant embryogenic callus (EC) is an irregular embryogenic cell mass with strong regenerative ability that can be used for propagation and genetic transformation. However, difficulties with EC induction have hindered the breeding of drumstick, a tree with diverse potential commercial uses. In this study, three drumstick EC cDNA libraries were sequenced using an Illumina NovaSeq 6000 system. A total of 7191 differentially expressed genes (DEGs) for embryogenic callus development were identified, of which 2325 were mapped to the KEGG database, with the categories of plant hormone signal transduction and Plant-pathogen interaction being well-represented. The results obtained suggest that auxin and cytokinin metabolism and several embryogenesis-labeled genes are involved in embryogenic callus induction. Additionally, 589 transcription factors from 20 different families were differentially expressed during EC formation. The differential expression of 16 unigenes related to auxin signaling pathways was validated experimentally by quantitative real time PCR (qRT-PCR) using samples representing three sequential developmental stages of drumstick EC, supporting their apparent involvement in drumstick EC formation. Our study provides valuable information about the molecular mechanism of EC formation and has revealed new genes involved in this process.Endian YangMingyang ZhengXuan ZouXiaoling HuangHeyue YangXiaoyang ChenJunjie ZhangMDPI AGarticle<i>Moringa oleifera</i>embryogenic callustranscriptomedifferentially expressed genesBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 12130, p 12130 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Moringa oleifera</i> embryogenic callus transcriptome differentially expressed genes Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	<i>Moringa oleifera</i> embryogenic callus transcriptome differentially expressed genes Biology (General) QH301-705.5 Chemistry QD1-999 Endian Yang Mingyang Zheng Xuan Zou Xiaoling Huang Heyue Yang Xiaoyang Chen Junjie Zhang Global Transcriptomic Analysis Reveals Differentially Expressed Genes Involved in Embryogenic Callus Induction in Drumstick (<i>Moringa oleifera</i> Lam.)
description	The plant embryogenic callus (EC) is an irregular embryogenic cell mass with strong regenerative ability that can be used for propagation and genetic transformation. However, difficulties with EC induction have hindered the breeding of drumstick, a tree with diverse potential commercial uses. In this study, three drumstick EC cDNA libraries were sequenced using an Illumina NovaSeq 6000 system. A total of 7191 differentially expressed genes (DEGs) for embryogenic callus development were identified, of which 2325 were mapped to the KEGG database, with the categories of plant hormone signal transduction and Plant-pathogen interaction being well-represented. The results obtained suggest that auxin and cytokinin metabolism and several embryogenesis-labeled genes are involved in embryogenic callus induction. Additionally, 589 transcription factors from 20 different families were differentially expressed during EC formation. The differential expression of 16 unigenes related to auxin signaling pathways was validated experimentally by quantitative real time PCR (qRT-PCR) using samples representing three sequential developmental stages of drumstick EC, supporting their apparent involvement in drumstick EC formation. Our study provides valuable information about the molecular mechanism of EC formation and has revealed new genes involved in this process.
format	article
author	Endian Yang Mingyang Zheng Xuan Zou Xiaoling Huang Heyue Yang Xiaoyang Chen Junjie Zhang
author_facet	Endian Yang Mingyang Zheng Xuan Zou Xiaoling Huang Heyue Yang Xiaoyang Chen Junjie Zhang
author_sort	Endian Yang
title	Global Transcriptomic Analysis Reveals Differentially Expressed Genes Involved in Embryogenic Callus Induction in Drumstick (<i>Moringa oleifera</i> Lam.)
title_short	Global Transcriptomic Analysis Reveals Differentially Expressed Genes Involved in Embryogenic Callus Induction in Drumstick (<i>Moringa oleifera</i> Lam.)
title_full	Global Transcriptomic Analysis Reveals Differentially Expressed Genes Involved in Embryogenic Callus Induction in Drumstick (<i>Moringa oleifera</i> Lam.)
title_fullStr	Global Transcriptomic Analysis Reveals Differentially Expressed Genes Involved in Embryogenic Callus Induction in Drumstick (<i>Moringa oleifera</i> Lam.)
title_full_unstemmed	Global Transcriptomic Analysis Reveals Differentially Expressed Genes Involved in Embryogenic Callus Induction in Drumstick (<i>Moringa oleifera</i> Lam.)
title_sort	global transcriptomic analysis reveals differentially expressed genes involved in embryogenic callus induction in drumstick (<i>moringa oleifera</i> lam.)
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/d300e2177e184322b00444c93be8d380
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Global Transcriptomic Analysis Reveals Differentially Expressed Genes Involved in Embryogenic Callus Induction in Drumstick (<i>Moringa oleifera</i> Lam.)

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