Defining the variety of cell types in developing and adult human kidneys by single-cell RNA sequencing

Abstract The kidney is among the most complex organs in terms of the variety of cell types. The cellular complexity of human kidneys is not fully unraveled and this challenge is further complicated by the existence of multiple progenitor pools and differentiation pathways. Researchers disagree on th...

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Autores principales:	A. Schumacher, M. B. Rookmaaker, J. A. Joles, R. Kramann, T. Q. Nguyen, M. van Griensven, V. L. S. LaPointe
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R
Acceso en línea:	https://doaj.org/article/d3043d76708045a19c3f96ec743a1615
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spelling	oai:doaj.org-article:d3043d76708045a19c3f96ec743a16152021-12-02T15:08:41ZDefining the variety of cell types in developing and adult human kidneys by single-cell RNA sequencing10.1038/s41536-021-00156-w2057-3995https://doaj.org/article/d3043d76708045a19c3f96ec743a16152021-08-01T00:00:00Zhttps://doi.org/10.1038/s41536-021-00156-whttps://doaj.org/toc/2057-3995Abstract The kidney is among the most complex organs in terms of the variety of cell types. The cellular complexity of human kidneys is not fully unraveled and this challenge is further complicated by the existence of multiple progenitor pools and differentiation pathways. Researchers disagree on the variety of renal cell types due to a lack of research providing a comprehensive picture and the challenge to translate findings between species. To find an answer to the number of human renal cell types, we discuss research that used single-cell RNA sequencing on developing and adult human kidney tissue and compares these findings to the literature of the pre-single-cell RNA sequencing era. We find that these publications show major steps towards the discovery of novel cell types and intermediate cell stages as well as complex molecular signatures and lineage pathways throughout development. The variety of cell types remains variable in the single-cell literature, which is due to the limitations of the technique. Nevertheless, our analysis approaches an accumulated number of 41 identified cell populations of renal lineage and 32 of non-renal lineage in the adult kidney, and there is certainly much more to discover. There is still a need for a consensus on a variety of definitions and standards in single-cell RNA sequencing research, such as the definition of what is a cell type. Nevertheless, this early-stage research already proves to be of significant impact for both clinical and regenerative medicine, and shows potential to enhance the generation of sophisticated in vitro kidney tissue.A. SchumacherM. B. RookmaakerJ. A. JolesR. KramannT. Q. NguyenM. van GriensvenV. L. S. LaPointeNature PortfolioarticleMedicineRENnpj Regenerative Medicine, Vol 6, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R
spellingShingle	Medicine R A. Schumacher M. B. Rookmaaker J. A. Joles R. Kramann T. Q. Nguyen M. van Griensven V. L. S. LaPointe Defining the variety of cell types in developing and adult human kidneys by single-cell RNA sequencing
description	Abstract The kidney is among the most complex organs in terms of the variety of cell types. The cellular complexity of human kidneys is not fully unraveled and this challenge is further complicated by the existence of multiple progenitor pools and differentiation pathways. Researchers disagree on the variety of renal cell types due to a lack of research providing a comprehensive picture and the challenge to translate findings between species. To find an answer to the number of human renal cell types, we discuss research that used single-cell RNA sequencing on developing and adult human kidney tissue and compares these findings to the literature of the pre-single-cell RNA sequencing era. We find that these publications show major steps towards the discovery of novel cell types and intermediate cell stages as well as complex molecular signatures and lineage pathways throughout development. The variety of cell types remains variable in the single-cell literature, which is due to the limitations of the technique. Nevertheless, our analysis approaches an accumulated number of 41 identified cell populations of renal lineage and 32 of non-renal lineage in the adult kidney, and there is certainly much more to discover. There is still a need for a consensus on a variety of definitions and standards in single-cell RNA sequencing research, such as the definition of what is a cell type. Nevertheless, this early-stage research already proves to be of significant impact for both clinical and regenerative medicine, and shows potential to enhance the generation of sophisticated in vitro kidney tissue.
format	article
author	A. Schumacher M. B. Rookmaaker J. A. Joles R. Kramann T. Q. Nguyen M. van Griensven V. L. S. LaPointe
author_facet	A. Schumacher M. B. Rookmaaker J. A. Joles R. Kramann T. Q. Nguyen M. van Griensven V. L. S. LaPointe
author_sort	A. Schumacher
title	Defining the variety of cell types in developing and adult human kidneys by single-cell RNA sequencing
title_short	Defining the variety of cell types in developing and adult human kidneys by single-cell RNA sequencing
title_full	Defining the variety of cell types in developing and adult human kidneys by single-cell RNA sequencing
title_fullStr	Defining the variety of cell types in developing and adult human kidneys by single-cell RNA sequencing
title_full_unstemmed	Defining the variety of cell types in developing and adult human kidneys by single-cell RNA sequencing
title_sort	defining the variety of cell types in developing and adult human kidneys by single-cell rna sequencing
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/d3043d76708045a19c3f96ec743a1615
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Defining the variety of cell types in developing and adult human kidneys by single-cell RNA sequencing

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