A shape-adjusted ellipse approach corrects for varied axonal dispersion angles and myelination in primate nerve roots

Abstract Segmentation of axons in light and electron micrographs allows for quantitative high-resolution analysis of nervous tissues, but varied axonal dispersion angles result in over-estimates of fiber sizes. To overcome this technical challenge, we developed a novel shape-adjusted ellipse (SAE) d...

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Autores principales:	Petra M. Bartmeyer, Natalia P. Biscola, Leif A. Havton
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d308ae6b0d4644f4b4372080c4c76db5
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spelling	oai:doaj.org-article:d308ae6b0d4644f4b4372080c4c76db52021-12-02T10:44:22ZA shape-adjusted ellipse approach corrects for varied axonal dispersion angles and myelination in primate nerve roots10.1038/s41598-021-82575-92045-2322https://doaj.org/article/d308ae6b0d4644f4b4372080c4c76db52021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-82575-9https://doaj.org/toc/2045-2322Abstract Segmentation of axons in light and electron micrographs allows for quantitative high-resolution analysis of nervous tissues, but varied axonal dispersion angles result in over-estimates of fiber sizes. To overcome this technical challenge, we developed a novel shape-adjusted ellipse (SAE) determination of axonal size and myelination as an all-inclusive and non-biased tool to correct for oblique nerve fiber presentations. Our new resource was validated by light and electron microscopy against traditional methods of determining nerve fiber size and myelination in rhesus macaques as a model system. We performed detailed segmental mapping and characterized the morphological signatures of autonomic and motor fibers in primate lumbosacral ventral roots (VRs). An en bloc inter-subject variability for the preganglionic parasympathetic fibers within the L7-S2 VRs was determined. The SAE approach allows for morphological ground truth data collection and assignment of individual axons to functional phenotypes with direct implications for fiber mapping and neuromodulation studies.Petra M. BartmeyerNatalia P. BiscolaLeif A. HavtonNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Petra M. Bartmeyer Natalia P. Biscola Leif A. Havton A shape-adjusted ellipse approach corrects for varied axonal dispersion angles and myelination in primate nerve roots
description	Abstract Segmentation of axons in light and electron micrographs allows for quantitative high-resolution analysis of nervous tissues, but varied axonal dispersion angles result in over-estimates of fiber sizes. To overcome this technical challenge, we developed a novel shape-adjusted ellipse (SAE) determination of axonal size and myelination as an all-inclusive and non-biased tool to correct for oblique nerve fiber presentations. Our new resource was validated by light and electron microscopy against traditional methods of determining nerve fiber size and myelination in rhesus macaques as a model system. We performed detailed segmental mapping and characterized the morphological signatures of autonomic and motor fibers in primate lumbosacral ventral roots (VRs). An en bloc inter-subject variability for the preganglionic parasympathetic fibers within the L7-S2 VRs was determined. The SAE approach allows for morphological ground truth data collection and assignment of individual axons to functional phenotypes with direct implications for fiber mapping and neuromodulation studies.
format	article
author	Petra M. Bartmeyer Natalia P. Biscola Leif A. Havton
author_facet	Petra M. Bartmeyer Natalia P. Biscola Leif A. Havton
author_sort	Petra M. Bartmeyer
title	A shape-adjusted ellipse approach corrects for varied axonal dispersion angles and myelination in primate nerve roots
title_short	A shape-adjusted ellipse approach corrects for varied axonal dispersion angles and myelination in primate nerve roots
title_full	A shape-adjusted ellipse approach corrects for varied axonal dispersion angles and myelination in primate nerve roots
title_fullStr	A shape-adjusted ellipse approach corrects for varied axonal dispersion angles and myelination in primate nerve roots
title_full_unstemmed	A shape-adjusted ellipse approach corrects for varied axonal dispersion angles and myelination in primate nerve roots
title_sort	shape-adjusted ellipse approach corrects for varied axonal dispersion angles and myelination in primate nerve roots
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/d308ae6b0d4644f4b4372080c4c76db5
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A shape-adjusted ellipse approach corrects for varied axonal dispersion angles and myelination in primate nerve roots

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