Analysis of enzyme activity and microbial community structure changes in the anaerobic digestion process of cattle manure at sub-mesophilic temperatures

To improve the biogas potential in anaerobic digestion of cattle manure in cold regions, we investigated, in this study, the potential of the anaerobic digestion of cattle manure through sequencing batch anaerobic digestion experiments at sub-mesophilic temperatures (15°C, 20°C, 25°C, and 37°C) for...

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Autores principales:	Zhen Xiaofei, Luo Miao, Dong Haiying, Fang Lei, Wang Weiwei, Feng Lei, Yu Qin
Formato:	article
Lenguaje:	EN
Publicado:	De Gruyter 2021
Materias:	anaerobic digestion cattle manure enzyme activity biogas production microbial community structure Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/d30ca069ec0f4eeaa53476c8d1d43bde
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spelling	oai:doaj.org-article:d30ca069ec0f4eeaa53476c8d1d43bde2021-12-05T14:10:50ZAnalysis of enzyme activity and microbial community structure changes in the anaerobic digestion process of cattle manure at sub-mesophilic temperatures2191-955010.1515/gps-2021-0064https://doaj.org/article/d30ca069ec0f4eeaa53476c8d1d43bde2021-10-01T00:00:00Zhttps://doi.org/10.1515/gps-2021-0064https://doaj.org/toc/2191-9550To improve the biogas potential in anaerobic digestion of cattle manure in cold regions, we investigated, in this study, the potential of the anaerobic digestion of cattle manure through sequencing batch anaerobic digestion experiments at sub-mesophilic temperatures (15°C, 20°C, 25°C, and 37°C) for 50 days. Specifically, the changes in the biogas yield, pH, sCOD concentration, enzyme activity, and microbial community structure were examined. The maximum daily yield of biogas was 19.18 mL·gVS−1, which was recorded on day 6 at 37°C, and the final biogas accumulation yield at this temperature was 403.64 mL·gTS−1. The concentration of sCOD gradually increased as enzyme activity increased. The maximum activities of cellulase, hemicellulase, protease, and amylase were recorded in the 37°C experimental group. The decrease in temperature severely limited the activity of different types of enzymes, resulting in a decrease in the activity of microorganisms, which greatly influenced the methanogenic reaction. The dominant bacteria at the phylum level were Bacteroidetes and Proteobacteria, and the dominant methanogen at the genus level was Methanosaeta.Zhen XiaofeiLuo MiaoDong HaiyingFang LeiWang WeiweiFeng LeiYu QinDe Gruyterarticleanaerobic digestioncattle manureenzyme activitybiogas productionmicrobial community structureChemistryQD1-999ENGreen Processing and Synthesis, Vol 10, Iss 1, Pp 644-657 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	anaerobic digestion cattle manure enzyme activity biogas production microbial community structure Chemistry QD1-999
spellingShingle	anaerobic digestion cattle manure enzyme activity biogas production microbial community structure Chemistry QD1-999 Zhen Xiaofei Luo Miao Dong Haiying Fang Lei Wang Weiwei Feng Lei Yu Qin Analysis of enzyme activity and microbial community structure changes in the anaerobic digestion process of cattle manure at sub-mesophilic temperatures
description	To improve the biogas potential in anaerobic digestion of cattle manure in cold regions, we investigated, in this study, the potential of the anaerobic digestion of cattle manure through sequencing batch anaerobic digestion experiments at sub-mesophilic temperatures (15°C, 20°C, 25°C, and 37°C) for 50 days. Specifically, the changes in the biogas yield, pH, sCOD concentration, enzyme activity, and microbial community structure were examined. The maximum daily yield of biogas was 19.18 mL·gVS−1, which was recorded on day 6 at 37°C, and the final biogas accumulation yield at this temperature was 403.64 mL·gTS−1. The concentration of sCOD gradually increased as enzyme activity increased. The maximum activities of cellulase, hemicellulase, protease, and amylase were recorded in the 37°C experimental group. The decrease in temperature severely limited the activity of different types of enzymes, resulting in a decrease in the activity of microorganisms, which greatly influenced the methanogenic reaction. The dominant bacteria at the phylum level were Bacteroidetes and Proteobacteria, and the dominant methanogen at the genus level was Methanosaeta.
format	article
author	Zhen Xiaofei Luo Miao Dong Haiying Fang Lei Wang Weiwei Feng Lei Yu Qin
author_facet	Zhen Xiaofei Luo Miao Dong Haiying Fang Lei Wang Weiwei Feng Lei Yu Qin
author_sort	Zhen Xiaofei
title	Analysis of enzyme activity and microbial community structure changes in the anaerobic digestion process of cattle manure at sub-mesophilic temperatures
title_short	Analysis of enzyme activity and microbial community structure changes in the anaerobic digestion process of cattle manure at sub-mesophilic temperatures
title_full	Analysis of enzyme activity and microbial community structure changes in the anaerobic digestion process of cattle manure at sub-mesophilic temperatures
title_fullStr	Analysis of enzyme activity and microbial community structure changes in the anaerobic digestion process of cattle manure at sub-mesophilic temperatures
title_full_unstemmed	Analysis of enzyme activity and microbial community structure changes in the anaerobic digestion process of cattle manure at sub-mesophilic temperatures
title_sort	analysis of enzyme activity and microbial community structure changes in the anaerobic digestion process of cattle manure at sub-mesophilic temperatures
publisher	De Gruyter
publishDate	2021
url	https://doaj.org/article/d30ca069ec0f4eeaa53476c8d1d43bde
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Analysis of enzyme activity and microbial community structure changes in the anaerobic digestion process of cattle manure at sub-mesophilic temperatures

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