ID1/ID3 mediate the contribution of skin fibroblasts to local nerve regeneration through Itga6 in wound repair

Abstract Cutaneous wound healing requires intricate synchronization of several key processes. Among them, local nerve regeneration is known to be vitally important for proper repair. However, the underlying mechanisms of local nerve regeneration are still unclear. Fibroblasts are one of the key cell...

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Autores principales:	Zelin Chen, Gufang Shen, Xu Tan, Langfan Qu, Can Zhang, Le Ma, Peng Luo, Xiaohui Cao, Fan Yang, Yunsheng Liu, Yu Wang, Chunmeng Shi
Formato:	article
Lenguaje:	EN
Publicado:	Wiley 2021
Materias:	fibroblasts nerve regeneration skin Medicine (General) R5-920 Cytology QH573-671
Acceso en línea:	https://doaj.org/article/d3120c3e012040d28cc8d6cfa49b419e
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spelling	oai:doaj.org-article:d3120c3e012040d28cc8d6cfa49b419e2021-12-03T12:11:46ZID1/ID3 mediate the contribution of skin fibroblasts to local nerve regeneration through Itga6 in wound repair2157-65802157-656410.1002/sctm.21-0093https://doaj.org/article/d3120c3e012040d28cc8d6cfa49b419e2021-12-01T00:00:00Zhttps://doi.org/10.1002/sctm.21-0093https://doaj.org/toc/2157-6564https://doaj.org/toc/2157-6580Abstract Cutaneous wound healing requires intricate synchronization of several key processes. Among them, local nerve regeneration is known to be vitally important for proper repair. However, the underlying mechanisms of local nerve regeneration are still unclear. Fibroblasts are one of the key cell types within the skin whose role in local nerve regeneration has not been extensively studied. In our study, we found skin fibroblasts were in tight contact with regenerated nerves during wound healing, while rare interactions were shown under normal circumstances. Moreover, skin fibroblasts surrounding the nerves were shown to be activated and reprogrammed to exhibit neural cell‐like properties by upregulated expressing inhibitor of DNA binding 1 (ID1) and ID3. Furthermore, we identified the regulation of integrin α6 (Itga6) by ID1/ID3 in fibroblasts as the mechanism for axon guidance. Accordingly, transplantation of the ID1/ID3‐overexpressing fibroblasts or topical injection of ID1/ID3 lentivirus significantly promoted local nerve regeneration and wound healing following skin excision or sciatic nerve injury. Therefore, we demonstrated a new role for skin fibroblasts in nerve regeneration following local injury by directly contacting and guiding axon regrowth, which might hold therapeutic potential in peripheral nerve disorders and peripheral neuropathies in relatively chronic refractory wounds.Zelin ChenGufang ShenXu TanLangfan QuCan ZhangLe MaPeng LuoXiaohui CaoFan YangYunsheng LiuYu WangChunmeng ShiWileyarticlefibroblastsnerveregenerationskinMedicine (General)R5-920CytologyQH573-671ENStem Cells Translational Medicine, Vol 10, Iss 12, Pp 1637-1649 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	fibroblasts nerve regeneration skin Medicine (General) R5-920 Cytology QH573-671
spellingShingle	fibroblasts nerve regeneration skin Medicine (General) R5-920 Cytology QH573-671 Zelin Chen Gufang Shen Xu Tan Langfan Qu Can Zhang Le Ma Peng Luo Xiaohui Cao Fan Yang Yunsheng Liu Yu Wang Chunmeng Shi ID1/ID3 mediate the contribution of skin fibroblasts to local nerve regeneration through Itga6 in wound repair
description	Abstract Cutaneous wound healing requires intricate synchronization of several key processes. Among them, local nerve regeneration is known to be vitally important for proper repair. However, the underlying mechanisms of local nerve regeneration are still unclear. Fibroblasts are one of the key cell types within the skin whose role in local nerve regeneration has not been extensively studied. In our study, we found skin fibroblasts were in tight contact with regenerated nerves during wound healing, while rare interactions were shown under normal circumstances. Moreover, skin fibroblasts surrounding the nerves were shown to be activated and reprogrammed to exhibit neural cell‐like properties by upregulated expressing inhibitor of DNA binding 1 (ID1) and ID3. Furthermore, we identified the regulation of integrin α6 (Itga6) by ID1/ID3 in fibroblasts as the mechanism for axon guidance. Accordingly, transplantation of the ID1/ID3‐overexpressing fibroblasts or topical injection of ID1/ID3 lentivirus significantly promoted local nerve regeneration and wound healing following skin excision or sciatic nerve injury. Therefore, we demonstrated a new role for skin fibroblasts in nerve regeneration following local injury by directly contacting and guiding axon regrowth, which might hold therapeutic potential in peripheral nerve disorders and peripheral neuropathies in relatively chronic refractory wounds.
format	article
author	Zelin Chen Gufang Shen Xu Tan Langfan Qu Can Zhang Le Ma Peng Luo Xiaohui Cao Fan Yang Yunsheng Liu Yu Wang Chunmeng Shi
author_facet	Zelin Chen Gufang Shen Xu Tan Langfan Qu Can Zhang Le Ma Peng Luo Xiaohui Cao Fan Yang Yunsheng Liu Yu Wang Chunmeng Shi
author_sort	Zelin Chen
title	ID1/ID3 mediate the contribution of skin fibroblasts to local nerve regeneration through Itga6 in wound repair
title_short	ID1/ID3 mediate the contribution of skin fibroblasts to local nerve regeneration through Itga6 in wound repair
title_full	ID1/ID3 mediate the contribution of skin fibroblasts to local nerve regeneration through Itga6 in wound repair
title_fullStr	ID1/ID3 mediate the contribution of skin fibroblasts to local nerve regeneration through Itga6 in wound repair
title_full_unstemmed	ID1/ID3 mediate the contribution of skin fibroblasts to local nerve regeneration through Itga6 in wound repair
title_sort	id1/id3 mediate the contribution of skin fibroblasts to local nerve regeneration through itga6 in wound repair
publisher	Wiley
publishDate	2021
url	https://doaj.org/article/d3120c3e012040d28cc8d6cfa49b419e
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ID1/ID3 mediate the contribution of skin fibroblasts to local nerve regeneration through Itga6 in wound repair

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