Ascomycetes versus Spent Mushroom Substrate in Mycoremediation of Dredged Sediments Contaminated by Total Petroleum Hydrocarbons: The Involvement of the Bacterial Metabolism

Two mycoremediation approaches for the depletion of the total petroleum hydrocarbons in dredged sediments were compared: co-composting with spent mushroom substrate (SMS) from Pleurotus ostreatus and bioaugmentation with Lambertella sp. MUT 5852, an ascomycetes...

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Autores principales:	Simone Becarelli, Giovanna Siracusa, Ilaria Chicca, Giacomo Bernabei, Simona Di Gregorio
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	mycoremediation <i>Lambertella</i> sp. MUT 5852 <i>Pleurotus ostreatus</i> predictive functional metagenomic analysis synergism between fungi and bacteria total petroleum hydrocarbon depletion Hydraulic engineering TC1-978 Water supply for domestic and industrial purposes TD201-500
Acceso en línea:	https://doaj.org/article/d31b8ffb7b2046e084316b37954f14f2
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spelling	oai:doaj.org-article:d31b8ffb7b2046e084316b37954f14f22021-11-11T19:55:32ZAscomycetes <i>versus</i> Spent Mushroom Substrate in Mycoremediation of Dredged Sediments Contaminated by Total Petroleum Hydrocarbons: The Involvement of the Bacterial Metabolism10.3390/w132130402073-4441https://doaj.org/article/d31b8ffb7b2046e084316b37954f14f22021-11-01T00:00:00Zhttps://www.mdpi.com/2073-4441/13/21/3040https://doaj.org/toc/2073-4441Two mycoremediation approaches for the depletion of the total petroleum hydrocarbons in dredged sediments were compared: co-composting with spent mushroom substrate (SMS) from <i>Pleurotus ostreatus</i> and bioaugmentation with <i>Lambertella</i> sp. MUT 5852, an ascomycetes autochthonous to the sediment, capable of utilizing diesel oil its sole carbon source. After 28 days of incubation, 99% depletion was observed in presence of <i>Lambertella</i> sp. MUT 5852. No total petroleum hydrocarbon depletion was observed in sediment co-composting with the SMS after 60 days of incubation. 16S rDNA metabarcoding of the bacterial community was performed to evaluate the potential synergism between fungi and bacteria in the bioremediation process. The functional metagenomic prediction approach indicated that the biodiversity of the bacterial genera potentially involved in the degradation of TPH was higher in sediment bioaugmented with <i>Lambertella</i> sp. MUT 5852, which resulted in being mandatory for TPH depletion. Mechanisms of co-substrate inhibition of the hydrocarburoclastic bacterial species, due to the bioavailable organic matter of the SMS, are suggested to be involved in the observed kinetics of TPH depletion, failing in the case of SMS and successful in the case of <i>Lambertella</i> sp. MUT 5852.Simone BecarelliGiovanna SiracusaIlaria ChiccaGiacomo BernabeiSimona Di GregorioMDPI AGarticlemycoremediation<i>Lambertella</i> sp. MUT 5852<i>Pleurotus ostreatus</i>predictive functional metagenomic analysissynergism between fungi and bacteriatotal petroleum hydrocarbon depletionHydraulic engineeringTC1-978Water supply for domestic and industrial purposesTD201-500ENWater, Vol 13, Iss 3040, p 3040 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	mycoremediation <i>Lambertella</i> sp. MUT 5852 <i>Pleurotus ostreatus</i> predictive functional metagenomic analysis synergism between fungi and bacteria total petroleum hydrocarbon depletion Hydraulic engineering TC1-978 Water supply for domestic and industrial purposes TD201-500
spellingShingle	mycoremediation <i>Lambertella</i> sp. MUT 5852 <i>Pleurotus ostreatus</i> predictive functional metagenomic analysis synergism between fungi and bacteria total petroleum hydrocarbon depletion Hydraulic engineering TC1-978 Water supply for domestic and industrial purposes TD201-500 Simone Becarelli Giovanna Siracusa Ilaria Chicca Giacomo Bernabei Simona Di Gregorio Ascomycetes <i>versus</i> Spent Mushroom Substrate in Mycoremediation of Dredged Sediments Contaminated by Total Petroleum Hydrocarbons: The Involvement of the Bacterial Metabolism
description	Two mycoremediation approaches for the depletion of the total petroleum hydrocarbons in dredged sediments were compared: co-composting with spent mushroom substrate (SMS) from <i>Pleurotus ostreatus</i> and bioaugmentation with <i>Lambertella</i> sp. MUT 5852, an ascomycetes autochthonous to the sediment, capable of utilizing diesel oil its sole carbon source. After 28 days of incubation, 99% depletion was observed in presence of <i>Lambertella</i> sp. MUT 5852. No total petroleum hydrocarbon depletion was observed in sediment co-composting with the SMS after 60 days of incubation. 16S rDNA metabarcoding of the bacterial community was performed to evaluate the potential synergism between fungi and bacteria in the bioremediation process. The functional metagenomic prediction approach indicated that the biodiversity of the bacterial genera potentially involved in the degradation of TPH was higher in sediment bioaugmented with <i>Lambertella</i> sp. MUT 5852, which resulted in being mandatory for TPH depletion. Mechanisms of co-substrate inhibition of the hydrocarburoclastic bacterial species, due to the bioavailable organic matter of the SMS, are suggested to be involved in the observed kinetics of TPH depletion, failing in the case of SMS and successful in the case of <i>Lambertella</i> sp. MUT 5852.
format	article
author	Simone Becarelli Giovanna Siracusa Ilaria Chicca Giacomo Bernabei Simona Di Gregorio
author_facet	Simone Becarelli Giovanna Siracusa Ilaria Chicca Giacomo Bernabei Simona Di Gregorio
author_sort	Simone Becarelli
title	Ascomycetes <i>versus</i> Spent Mushroom Substrate in Mycoremediation of Dredged Sediments Contaminated by Total Petroleum Hydrocarbons: The Involvement of the Bacterial Metabolism
title_short	Ascomycetes <i>versus</i> Spent Mushroom Substrate in Mycoremediation of Dredged Sediments Contaminated by Total Petroleum Hydrocarbons: The Involvement of the Bacterial Metabolism
title_full	Ascomycetes <i>versus</i> Spent Mushroom Substrate in Mycoremediation of Dredged Sediments Contaminated by Total Petroleum Hydrocarbons: The Involvement of the Bacterial Metabolism
title_fullStr	Ascomycetes <i>versus</i> Spent Mushroom Substrate in Mycoremediation of Dredged Sediments Contaminated by Total Petroleum Hydrocarbons: The Involvement of the Bacterial Metabolism
title_full_unstemmed	Ascomycetes <i>versus</i> Spent Mushroom Substrate in Mycoremediation of Dredged Sediments Contaminated by Total Petroleum Hydrocarbons: The Involvement of the Bacterial Metabolism
title_sort	ascomycetes <i>versus</i> spent mushroom substrate in mycoremediation of dredged sediments contaminated by total petroleum hydrocarbons: the involvement of the bacterial metabolism
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/d31b8ffb7b2046e084316b37954f14f2
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Ascomycetes <i>versus</i> Spent Mushroom Substrate in Mycoremediation of Dredged Sediments Contaminated by Total Petroleum Hydrocarbons: The Involvement of the Bacterial Metabolism

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