High Temporal Resolution 3D Live-Cell Imaging of Budding Yeast Meiosis Defines Discontinuous Actin/Telomere-Mediated Chromosome Motion, Correlated Nuclear Envelope Deformation and Actin Filament Dynamics

Chromosome movement is prominent at mid-meiotic prophase and is proposed to enhance the efficiency and/or stringency of homolog pairing and/or to help prevent or resolve topological entanglements. Here, we combine fluorescent repressor operator system (FROS) labeling with three-dimensional (3D) live...

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Autores principales:	Tadasu Nozaki, Frederick Chang, Beth Weiner, Nancy Kleckner
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	3D time-lapse imaging chromosome motion FROS telomere actin homolog pairing Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/d32a1535efb34e5f8ba30c547e1e90d8
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spelling	oai:doaj.org-article:d32a1535efb34e5f8ba30c547e1e90d82021-12-01T01:50:44ZHigh Temporal Resolution 3D Live-Cell Imaging of Budding Yeast Meiosis Defines Discontinuous Actin/Telomere-Mediated Chromosome Motion, Correlated Nuclear Envelope Deformation and Actin Filament Dynamics2296-634X10.3389/fcell.2021.687132https://doaj.org/article/d32a1535efb34e5f8ba30c547e1e90d82021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fcell.2021.687132/fullhttps://doaj.org/toc/2296-634XChromosome movement is prominent at mid-meiotic prophase and is proposed to enhance the efficiency and/or stringency of homolog pairing and/or to help prevent or resolve topological entanglements. Here, we combine fluorescent repressor operator system (FROS) labeling with three-dimensional (3D) live-cell imaging at high spatio-temporal resolution to define the detailed kinetics of mid-meiotic prophase motion for a single telomere-proximal locus in budding yeast. Telomere motions can be grouped into three general categories: (i) pauses, in which the telomere “jiggles in place”; (ii) rapid, straight/curvilinear motion which reflects Myo2/actin-mediated transport of the monitored telomere; and (iii) slower directional motions, most of which likely reflect indirectly promoted motion of the monitored telomere in coordination with actin-mediated motion of an unmarked telomere. These and other findings highlight the importance of dynamic assembly/disassembly of telomere/LINC/actin ensembles and also suggest important roles for nuclear envelope deformations promoted by actin-mediated telomere/LINC movement. The presented low-SNR (signal-to-noise ratio) imaging methodology provides opportunities for future exploration of homolog pairing and related phenomena.Tadasu NozakiFrederick ChangBeth WeinerNancy KlecknerFrontiers Media S.A.article3D time-lapse imagingchromosome motionFROStelomereactinhomolog pairingBiology (General)QH301-705.5ENFrontiers in Cell and Developmental Biology, Vol 9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	3D time-lapse imaging chromosome motion FROS telomere actin homolog pairing Biology (General) QH301-705.5
spellingShingle	3D time-lapse imaging chromosome motion FROS telomere actin homolog pairing Biology (General) QH301-705.5 Tadasu Nozaki Frederick Chang Beth Weiner Nancy Kleckner High Temporal Resolution 3D Live-Cell Imaging of Budding Yeast Meiosis Defines Discontinuous Actin/Telomere-Mediated Chromosome Motion, Correlated Nuclear Envelope Deformation and Actin Filament Dynamics
description	Chromosome movement is prominent at mid-meiotic prophase and is proposed to enhance the efficiency and/or stringency of homolog pairing and/or to help prevent or resolve topological entanglements. Here, we combine fluorescent repressor operator system (FROS) labeling with three-dimensional (3D) live-cell imaging at high spatio-temporal resolution to define the detailed kinetics of mid-meiotic prophase motion for a single telomere-proximal locus in budding yeast. Telomere motions can be grouped into three general categories: (i) pauses, in which the telomere “jiggles in place”; (ii) rapid, straight/curvilinear motion which reflects Myo2/actin-mediated transport of the monitored telomere; and (iii) slower directional motions, most of which likely reflect indirectly promoted motion of the monitored telomere in coordination with actin-mediated motion of an unmarked telomere. These and other findings highlight the importance of dynamic assembly/disassembly of telomere/LINC/actin ensembles and also suggest important roles for nuclear envelope deformations promoted by actin-mediated telomere/LINC movement. The presented low-SNR (signal-to-noise ratio) imaging methodology provides opportunities for future exploration of homolog pairing and related phenomena.
format	article
author	Tadasu Nozaki Frederick Chang Beth Weiner Nancy Kleckner
author_facet	Tadasu Nozaki Frederick Chang Beth Weiner Nancy Kleckner
author_sort	Tadasu Nozaki
title	High Temporal Resolution 3D Live-Cell Imaging of Budding Yeast Meiosis Defines Discontinuous Actin/Telomere-Mediated Chromosome Motion, Correlated Nuclear Envelope Deformation and Actin Filament Dynamics
title_short	High Temporal Resolution 3D Live-Cell Imaging of Budding Yeast Meiosis Defines Discontinuous Actin/Telomere-Mediated Chromosome Motion, Correlated Nuclear Envelope Deformation and Actin Filament Dynamics
title_full	High Temporal Resolution 3D Live-Cell Imaging of Budding Yeast Meiosis Defines Discontinuous Actin/Telomere-Mediated Chromosome Motion, Correlated Nuclear Envelope Deformation and Actin Filament Dynamics
title_fullStr	High Temporal Resolution 3D Live-Cell Imaging of Budding Yeast Meiosis Defines Discontinuous Actin/Telomere-Mediated Chromosome Motion, Correlated Nuclear Envelope Deformation and Actin Filament Dynamics
title_full_unstemmed	High Temporal Resolution 3D Live-Cell Imaging of Budding Yeast Meiosis Defines Discontinuous Actin/Telomere-Mediated Chromosome Motion, Correlated Nuclear Envelope Deformation and Actin Filament Dynamics
title_sort	high temporal resolution 3d live-cell imaging of budding yeast meiosis defines discontinuous actin/telomere-mediated chromosome motion, correlated nuclear envelope deformation and actin filament dynamics
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/d32a1535efb34e5f8ba30c547e1e90d8
work_keys_str_mv	AT tadasunozaki hightemporalresolution3dlivecellimagingofbuddingyeastmeiosisdefinesdiscontinuousactintelomeremediatedchromosomemotioncorrelatednuclearenvelopedeformationandactinfilamentdynamics AT frederickchang hightemporalresolution3dlivecellimagingofbuddingyeastmeiosisdefinesdiscontinuousactintelomeremediatedchromosomemotioncorrelatednuclearenvelopedeformationandactinfilamentdynamics AT bethweiner hightemporalresolution3dlivecellimagingofbuddingyeastmeiosisdefinesdiscontinuousactintelomeremediatedchromosomemotioncorrelatednuclearenvelopedeformationandactinfilamentdynamics AT nancykleckner hightemporalresolution3dlivecellimagingofbuddingyeastmeiosisdefinesdiscontinuousactintelomeremediatedchromosomemotioncorrelatednuclearenvelopedeformationandactinfilamentdynamics
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High Temporal Resolution 3D Live-Cell Imaging of Budding Yeast Meiosis Defines Discontinuous Actin/Telomere-Mediated Chromosome Motion, Correlated Nuclear Envelope Deformation and Actin Filament Dynamics

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