Effect of silencing HDAC9 on the proliferation and differentiation of periodontal ligament stem cells

Objective To investigate the effect of silencing histone deacetylase 9 (HDAC9) expression on the proliferation and osteogenic differentiation of periodontal ligament stem cells (PDLSCs). Methods PDLSCs were isolated, cultured and identified in vitro. An siRNA construct specific for HDAC9 was transfe...

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Autores principales:	GE Wenbin, ZHANG Kun, LUO Shitong, ZHOU Zhi, LIU Yali
Formato:	article
Lenguaje:	ZH
Publicado:	Editorial Department of Journal of Prevention and Treatment for Stomatological Diseases 2022
Materias:	histone deacetylase 9 periodontal ligament stem cells proliferation osteogenic differentiation proliferating cell nuclear antigen mineralized nodules runt-related transcription factor 2 alkaline phosphatase Medicine R
Acceso en línea:	https://doaj.org/article/d32b0329ee7a465aac274ee432608829
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spelling	oai:doaj.org-article:d32b0329ee7a465aac274ee4326088292021-11-25T07:09:34ZEffect of silencing HDAC9 on the proliferation and differentiation of periodontal ligament stem cells10.12016/j.issn.2096-1456.2022.02.0032096-1456https://doaj.org/article/d32b0329ee7a465aac274ee4326088292022-02-01T00:00:00Zhttp://www.kqjbfz.com/CN/10.12016/j.issn.2096-1456.2022.02.003https://doaj.org/toc/2096-1456Objective To investigate the effect of silencing histone deacetylase 9 (HDAC9) expression on the proliferation and osteogenic differentiation of periodontal ligament stem cells (PDLSCs). Methods PDLSCs were isolated, cultured and identified in vitro. An siRNA construct specific for HDAC9 was transfected into PDLSCs (siHDAC9 group), and a nontargeting siRNA was used as a control (siNC group). The interference effect was determined by qRT-PCR. The cell cycle progression of PDLSCs was detected using flow cytometry. The proliferation activity of PDLSCs was detected via CCK-8 assay. Western blotting was used to detect the protein expression of proliferating cell nuclear antigen (PCNA). The mRNA expression of runt-related transcription factor 2 (RUNX2) and alkaline phosphatase (ALP) was investigated by qRT-PCR. The protein expression of RUNX2 was detected by western blotting. In addition, the formation of mineralized nodules was assessed by alizarin red staining. Results Compared with that in the siNC group, the mRNA expression of HDAC9 in the siHDAC9 group was lower (P < 0.01). Moreover, compared with those in the siNC group, the proliferation index (P<0.01), proliferation activity (P<0.05) and protein expression of PCNA (P<0.01) in the siHDAC9 group were all increased. Compared with the siNC group, the siHDAC9 group exhibited higher mRNA expression of RUNX2 and ALP (P < 0.05), and the protein expression of RUNX2 showed the same results (P < 0.01). The results of alizarin red staining showed that compared to the siNC group, the siHDAC9 group formed more mineralized nodules. Conclusion Silencing HDAC9 expression can promote the proliferation and osteogenic differentiation of PDLSCs.GE WenbinZHANG KunLUO ShitongZHOU ZhiLIU YaliEditorial Department of Journal of Prevention and Treatment for Stomatological Diseasesarticlehistone deacetylase 9periodontal ligament stem cellsproliferationosteogenic differentiationproliferating cell nuclear antigenmineralized nodulesrunt-related transcription factor 2alkaline phosphataseMedicineRZH口腔疾病防治, Vol 30, Iss 2, Pp 89-96 (2022)
institution	DOAJ
collection	DOAJ
language	ZH
topic	histone deacetylase 9 periodontal ligament stem cells proliferation osteogenic differentiation proliferating cell nuclear antigen mineralized nodules runt-related transcription factor 2 alkaline phosphatase Medicine R
spellingShingle	histone deacetylase 9 periodontal ligament stem cells proliferation osteogenic differentiation proliferating cell nuclear antigen mineralized nodules runt-related transcription factor 2 alkaline phosphatase Medicine R GE Wenbin ZHANG Kun LUO Shitong ZHOU Zhi LIU Yali Effect of silencing HDAC9 on the proliferation and differentiation of periodontal ligament stem cells
description	Objective To investigate the effect of silencing histone deacetylase 9 (HDAC9) expression on the proliferation and osteogenic differentiation of periodontal ligament stem cells (PDLSCs). Methods PDLSCs were isolated, cultured and identified in vitro. An siRNA construct specific for HDAC9 was transfected into PDLSCs (siHDAC9 group), and a nontargeting siRNA was used as a control (siNC group). The interference effect was determined by qRT-PCR. The cell cycle progression of PDLSCs was detected using flow cytometry. The proliferation activity of PDLSCs was detected via CCK-8 assay. Western blotting was used to detect the protein expression of proliferating cell nuclear antigen (PCNA). The mRNA expression of runt-related transcription factor 2 (RUNX2) and alkaline phosphatase (ALP) was investigated by qRT-PCR. The protein expression of RUNX2 was detected by western blotting. In addition, the formation of mineralized nodules was assessed by alizarin red staining. Results Compared with that in the siNC group, the mRNA expression of HDAC9 in the siHDAC9 group was lower (P < 0.01). Moreover, compared with those in the siNC group, the proliferation index (P<0.01), proliferation activity (P<0.05) and protein expression of PCNA (P<0.01) in the siHDAC9 group were all increased. Compared with the siNC group, the siHDAC9 group exhibited higher mRNA expression of RUNX2 and ALP (P < 0.05), and the protein expression of RUNX2 showed the same results (P < 0.01). The results of alizarin red staining showed that compared to the siNC group, the siHDAC9 group formed more mineralized nodules. Conclusion Silencing HDAC9 expression can promote the proliferation and osteogenic differentiation of PDLSCs.
format	article
author	GE Wenbin ZHANG Kun LUO Shitong ZHOU Zhi LIU Yali
author_facet	GE Wenbin ZHANG Kun LUO Shitong ZHOU Zhi LIU Yali
author_sort	GE Wenbin
title	Effect of silencing HDAC9 on the proliferation and differentiation of periodontal ligament stem cells
title_short	Effect of silencing HDAC9 on the proliferation and differentiation of periodontal ligament stem cells
title_full	Effect of silencing HDAC9 on the proliferation and differentiation of periodontal ligament stem cells
title_fullStr	Effect of silencing HDAC9 on the proliferation and differentiation of periodontal ligament stem cells
title_full_unstemmed	Effect of silencing HDAC9 on the proliferation and differentiation of periodontal ligament stem cells
title_sort	effect of silencing hdac9 on the proliferation and differentiation of periodontal ligament stem cells
publisher	Editorial Department of Journal of Prevention and Treatment for Stomatological Diseases
publishDate	2022
url	https://doaj.org/article/d32b0329ee7a465aac274ee432608829
work_keys_str_mv	AT gewenbin effectofsilencinghdac9ontheproliferationanddifferentiationofperiodontalligamentstemcells AT zhangkun effectofsilencinghdac9ontheproliferationanddifferentiationofperiodontalligamentstemcells AT luoshitong effectofsilencinghdac9ontheproliferationanddifferentiationofperiodontalligamentstemcells AT zhouzhi effectofsilencinghdac9ontheproliferationanddifferentiationofperiodontalligamentstemcells AT liuyali effectofsilencinghdac9ontheproliferationanddifferentiationofperiodontalligamentstemcells
_version_	1718413649294393344

Effect of silencing HDAC9 on the proliferation and differentiation of periodontal ligament stem cells

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