Electrospun fiber membranes enable proliferation of genetically modified cells

Mandula Borjigin*, Chris Eskridge*, Rohina Niamat, Bryan Strouse, Pawel Bialk, Eric B KmiecDepartment of Chemistry, Delaware State University, Dover, DE, USA *These authors contributed equally to this work Abstract: Polycaprolactone (PCL) and its blended composites (chitosan, gelatin, and lecithin)...

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Autores principales:	Borjigin M, Eskridge C, Niamat R, Strouse B, Bialk P, Kmiec EB
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2013
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/d33029c3ff6941f78faefea7c8fb08a9
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spelling	oai:doaj.org-article:d33029c3ff6941f78faefea7c8fb08a92021-12-02T08:08:31ZElectrospun fiber membranes enable proliferation of genetically modified cells1176-91141178-2013https://doaj.org/article/d33029c3ff6941f78faefea7c8fb08a92013-02-01T00:00:00Zhttp://www.dovepress.com/electrospun-fiber-membranes-enable-proliferation-of-genetically-modifi-a12312https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Mandula Borjigin, Chris Eskridge, Rohina Niamat, Bryan Strouse, Pawel Bialk, Eric B KmiecDepartment of Chemistry, Delaware State University, Dover, DE, USA *These authors contributed equally to this work Abstract: Polycaprolactone (PCL) and its blended composites (chitosan, gelatin, and lecithin) are well-established biomaterials that can enrich cell growth and enable tissue engineering. However, their application in the recovery and proliferation of genetically modified cells has not been studied. In the study reported here, we fabricated PCL-biomaterial blended fiber membranes, characterized them using physicochemical techniques, and used them as templates for the growth of genetically modified HCT116-19 colon cancer cells. Our data show that the blended polymers are highly miscible and form homogenous electrospun fiber membranes of uniform texture. The aligned PCL nanofibers support robust cell growth, yielding a 2.5-fold higher proliferation rate than cells plated on standard plastic plate surfaces. PCL-lecithin fiber membranes yielded a 2.7-fold higher rate of proliferation, while PCL-chitosan supported a more modest growth rate (1.5-fold higher). Surprisingly, PCL-gelatin did not enhance cell proliferation when compared to the rate of cell growth on plastic surfaces. Keywords: nanofibers, PCL-biomaterial blends, miscibility, gene editing, cell proliferationBorjigin MEskridge CNiamat RStrouse BBialk PKmiec EBDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2013, Iss default, Pp 855-864 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Borjigin M Eskridge C Niamat R Strouse B Bialk P Kmiec EB Electrospun fiber membranes enable proliferation of genetically modified cells
description	Mandula Borjigin, Chris Eskridge, Rohina Niamat, Bryan Strouse, Pawel Bialk, Eric B KmiecDepartment of Chemistry, Delaware State University, Dover, DE, USA *These authors contributed equally to this work Abstract: Polycaprolactone (PCL) and its blended composites (chitosan, gelatin, and lecithin) are well-established biomaterials that can enrich cell growth and enable tissue engineering. However, their application in the recovery and proliferation of genetically modified cells has not been studied. In the study reported here, we fabricated PCL-biomaterial blended fiber membranes, characterized them using physicochemical techniques, and used them as templates for the growth of genetically modified HCT116-19 colon cancer cells. Our data show that the blended polymers are highly miscible and form homogenous electrospun fiber membranes of uniform texture. The aligned PCL nanofibers support robust cell growth, yielding a 2.5-fold higher proliferation rate than cells plated on standard plastic plate surfaces. PCL-lecithin fiber membranes yielded a 2.7-fold higher rate of proliferation, while PCL-chitosan supported a more modest growth rate (1.5-fold higher). Surprisingly, PCL-gelatin did not enhance cell proliferation when compared to the rate of cell growth on plastic surfaces. Keywords: nanofibers, PCL-biomaterial blends, miscibility, gene editing, cell proliferation
format	article
author	Borjigin M Eskridge C Niamat R Strouse B Bialk P Kmiec EB
author_facet	Borjigin M Eskridge C Niamat R Strouse B Bialk P Kmiec EB
author_sort	Borjigin M
title	Electrospun fiber membranes enable proliferation of genetically modified cells
title_short	Electrospun fiber membranes enable proliferation of genetically modified cells
title_full	Electrospun fiber membranes enable proliferation of genetically modified cells
title_fullStr	Electrospun fiber membranes enable proliferation of genetically modified cells
title_full_unstemmed	Electrospun fiber membranes enable proliferation of genetically modified cells
title_sort	electrospun fiber membranes enable proliferation of genetically modified cells
publisher	Dove Medical Press
publishDate	2013
url	https://doaj.org/article/d33029c3ff6941f78faefea7c8fb08a9
work_keys_str_mv	AT borjiginm electrospunfibermembranesenableproliferationofgeneticallymodifiedcells AT eskridgec electrospunfibermembranesenableproliferationofgeneticallymodifiedcells AT niamatr electrospunfibermembranesenableproliferationofgeneticallymodifiedcells AT strouseb electrospunfibermembranesenableproliferationofgeneticallymodifiedcells AT bialkp electrospunfibermembranesenableproliferationofgeneticallymodifiedcells AT kmieceb electrospunfibermembranesenableproliferationofgeneticallymodifiedcells
_version_	1718398628215652352

Electrospun fiber membranes enable proliferation of genetically modified cells

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