Polycistronic Artificial microRNA-Mediated Resistance to Cucumber Green Mottle Mosaic Virus in Cucumber

Cucumber green mottle mosaic virus (CGMMV), as a typical seed-borne virus, causes costly and devastating diseases in the vegetable trade worldwide. Genetic sources for resistance to CGMMV in cucurbits are limited, and environmentally safe approaches for curbing the accumulation and spread of seed-tr...

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Autores principales:	Shuo Miao, Chaoqiong Liang, Jianqiang Li, Barbara Baker, Laixin Luo
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	seed-borne virus cucumber green mottle mosaic virus RNA silencing suppressor RNA interference polycistronic artificial microRNA <i>Cucumis sativus</i> L. Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/d333386b4f10428fb7292471cd868330
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spelling	oai:doaj.org-article:d333386b4f10428fb7292471cd8683302021-11-25T17:54:38ZPolycistronic Artificial microRNA-Mediated Resistance to Cucumber Green Mottle Mosaic Virus in Cucumber10.3390/ijms2222122371422-00671661-6596https://doaj.org/article/d333386b4f10428fb7292471cd8683302021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/22/12237https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Cucumber green mottle mosaic virus (CGMMV), as a typical seed-borne virus, causes costly and devastating diseases in the vegetable trade worldwide. Genetic sources for resistance to CGMMV in cucurbits are limited, and environmentally safe approaches for curbing the accumulation and spread of seed-transmitted viruses and cultivating completely resistant plants are needed. Here, we describe the design and application of RNA interference-based technologies, containing artificial microRNA (amiRNA) and synthetic <i>trans</i>-acting small interfering RNA (syn-tasiRNA), against conserved regions of different strains of the CGMMV genome. We used a rapid transient sensor system to identify effective anti-CGMMV amiRNAs. A virus seed transmission assay was developed, showing that the externally added polycistronic amiRNA and syn-tasiRNA can successfully block the accumulation of CGMMV in cucumber, but different virulent strains exhibited distinct influences on the expression of amiRNA due to the activity of the RNA-silencing suppressor. We also established stable transgenic cucumber plants expressing polycistronic amiRNA, which conferred disease resistance against CGMMV, and no sequence mutation was observed in CGMMV. This study demonstrates that RNA interference-based technologies can effectively prevent the occurrence and accumulation of CGMMV. The results provide a basis to establish and fine-tune approaches to prevent and treat seed-based transmission viral infections.Shuo MiaoChaoqiong LiangJianqiang LiBarbara BakerLaixin LuoMDPI AGarticleseed-borne viruscucumber green mottle mosaic virusRNA silencing suppressorRNA interferencepolycistronic artificial microRNA<i>Cucumis sativus</i> L.Biology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 12237, p 12237 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	seed-borne virus cucumber green mottle mosaic virus RNA silencing suppressor RNA interference polycistronic artificial microRNA <i>Cucumis sativus</i> L. Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	seed-borne virus cucumber green mottle mosaic virus RNA silencing suppressor RNA interference polycistronic artificial microRNA <i>Cucumis sativus</i> L. Biology (General) QH301-705.5 Chemistry QD1-999 Shuo Miao Chaoqiong Liang Jianqiang Li Barbara Baker Laixin Luo Polycistronic Artificial microRNA-Mediated Resistance to Cucumber Green Mottle Mosaic Virus in Cucumber
description	Cucumber green mottle mosaic virus (CGMMV), as a typical seed-borne virus, causes costly and devastating diseases in the vegetable trade worldwide. Genetic sources for resistance to CGMMV in cucurbits are limited, and environmentally safe approaches for curbing the accumulation and spread of seed-transmitted viruses and cultivating completely resistant plants are needed. Here, we describe the design and application of RNA interference-based technologies, containing artificial microRNA (amiRNA) and synthetic <i>trans</i>-acting small interfering RNA (syn-tasiRNA), against conserved regions of different strains of the CGMMV genome. We used a rapid transient sensor system to identify effective anti-CGMMV amiRNAs. A virus seed transmission assay was developed, showing that the externally added polycistronic amiRNA and syn-tasiRNA can successfully block the accumulation of CGMMV in cucumber, but different virulent strains exhibited distinct influences on the expression of amiRNA due to the activity of the RNA-silencing suppressor. We also established stable transgenic cucumber plants expressing polycistronic amiRNA, which conferred disease resistance against CGMMV, and no sequence mutation was observed in CGMMV. This study demonstrates that RNA interference-based technologies can effectively prevent the occurrence and accumulation of CGMMV. The results provide a basis to establish and fine-tune approaches to prevent and treat seed-based transmission viral infections.
format	article
author	Shuo Miao Chaoqiong Liang Jianqiang Li Barbara Baker Laixin Luo
author_facet	Shuo Miao Chaoqiong Liang Jianqiang Li Barbara Baker Laixin Luo
author_sort	Shuo Miao
title	Polycistronic Artificial microRNA-Mediated Resistance to Cucumber Green Mottle Mosaic Virus in Cucumber
title_short	Polycistronic Artificial microRNA-Mediated Resistance to Cucumber Green Mottle Mosaic Virus in Cucumber
title_full	Polycistronic Artificial microRNA-Mediated Resistance to Cucumber Green Mottle Mosaic Virus in Cucumber
title_fullStr	Polycistronic Artificial microRNA-Mediated Resistance to Cucumber Green Mottle Mosaic Virus in Cucumber
title_full_unstemmed	Polycistronic Artificial microRNA-Mediated Resistance to Cucumber Green Mottle Mosaic Virus in Cucumber
title_sort	polycistronic artificial microrna-mediated resistance to cucumber green mottle mosaic virus in cucumber
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/d333386b4f10428fb7292471cd868330
work_keys_str_mv	AT shuomiao polycistronicartificialmicrornamediatedresistancetocucumbergreenmottlemosaicvirusincucumber AT chaoqiongliang polycistronicartificialmicrornamediatedresistancetocucumbergreenmottlemosaicvirusincucumber AT jianqiangli polycistronicartificialmicrornamediatedresistancetocucumbergreenmottlemosaicvirusincucumber AT barbarabaker polycistronicartificialmicrornamediatedresistancetocucumbergreenmottlemosaicvirusincucumber AT laixinluo polycistronicartificialmicrornamediatedresistancetocucumbergreenmottlemosaicvirusincucumber
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Polycistronic Artificial microRNA-Mediated Resistance to Cucumber Green Mottle Mosaic Virus in Cucumber

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