Genetic analysis and QTL mapping of seed coat color in sesame (Sesamum indicum L.).

Seed coat color is an important agronomic trait in sesame, as it is associated with seed biochemical properties, antioxidant content and activity and even disease resistance of sesame. Here, using a high-density linkage map, we analyzed genetic segregation and quantitative trait loci (QTL) for sesam...

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Autores principales:	Haiyang Zhang, Hongmei Miao, Libin Wei, Chun Li, Ruihong Zhao, Cuiying Wang
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d33d086a6dbe4a04ab194b6d41bf2b96
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spelling	oai:doaj.org-article:d33d086a6dbe4a04ab194b6d41bf2b962021-11-18T07:44:56ZGenetic analysis and QTL mapping of seed coat color in sesame (Sesamum indicum L.).1932-620310.1371/journal.pone.0063898https://doaj.org/article/d33d086a6dbe4a04ab194b6d41bf2b962013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23704951/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Seed coat color is an important agronomic trait in sesame, as it is associated with seed biochemical properties, antioxidant content and activity and even disease resistance of sesame. Here, using a high-density linkage map, we analyzed genetic segregation and quantitative trait loci (QTL) for sesame seed coat color in six generations (P1, P2, F1, BC1, BC2 and F2). Results showed that two major genes with additive-dominant-epistatic effects and polygenes with additive-dominant-epistatic effects were responsible for controlling the seed coat color trait. Average heritability of the major genes in the BC1, BC2 and F2 populations was 89.30%, 24.00%, and 91.11% respectively, while the heritability of polygenes was low in the BC1 (5.43%), in BC2 (0.00%) and in F2 (0.89%) populations. A high-density map was constructed using 724 polymorphic markers. 653 SSR, AFLP and RSAMPL loci were anchored in 14 linkage groups (LG) spanning a total of 1,216.00 cM. The average length of each LG was 86.86 cM and the marker density was 1.86 cM per marker interval. Four QTLs for seed coat color, QTL1-1, QTL11-1, QTL11-2 and QTL13-1, whose heritability ranged from 59.33%-69.89%, were detected in F3 populations using CIM and MCIM methods. Alleles at all QTLs from the black-seeded parent tended to increase the seed coat color. Results from QTLs mapping and classical genetic analysis among the P1, P2, F1, BC1, BC2 and F2 populations were comparatively consistent. This first QTL analysis and high-density genetic linkage map for sesame provided a good foundation for further research on sesame genetics and molecular marker-assisted selection (MAS).Haiyang ZhangHongmei MiaoLibin WeiChun LiRuihong ZhaoCuiying WangPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 5, p e63898 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Haiyang Zhang Hongmei Miao Libin Wei Chun Li Ruihong Zhao Cuiying Wang Genetic analysis and QTL mapping of seed coat color in sesame (Sesamum indicum L.).
description	Seed coat color is an important agronomic trait in sesame, as it is associated with seed biochemical properties, antioxidant content and activity and even disease resistance of sesame. Here, using a high-density linkage map, we analyzed genetic segregation and quantitative trait loci (QTL) for sesame seed coat color in six generations (P1, P2, F1, BC1, BC2 and F2). Results showed that two major genes with additive-dominant-epistatic effects and polygenes with additive-dominant-epistatic effects were responsible for controlling the seed coat color trait. Average heritability of the major genes in the BC1, BC2 and F2 populations was 89.30%, 24.00%, and 91.11% respectively, while the heritability of polygenes was low in the BC1 (5.43%), in BC2 (0.00%) and in F2 (0.89%) populations. A high-density map was constructed using 724 polymorphic markers. 653 SSR, AFLP and RSAMPL loci were anchored in 14 linkage groups (LG) spanning a total of 1,216.00 cM. The average length of each LG was 86.86 cM and the marker density was 1.86 cM per marker interval. Four QTLs for seed coat color, QTL1-1, QTL11-1, QTL11-2 and QTL13-1, whose heritability ranged from 59.33%-69.89%, were detected in F3 populations using CIM and MCIM methods. Alleles at all QTLs from the black-seeded parent tended to increase the seed coat color. Results from QTLs mapping and classical genetic analysis among the P1, P2, F1, BC1, BC2 and F2 populations were comparatively consistent. This first QTL analysis and high-density genetic linkage map for sesame provided a good foundation for further research on sesame genetics and molecular marker-assisted selection (MAS).
format	article
author	Haiyang Zhang Hongmei Miao Libin Wei Chun Li Ruihong Zhao Cuiying Wang
author_facet	Haiyang Zhang Hongmei Miao Libin Wei Chun Li Ruihong Zhao Cuiying Wang
author_sort	Haiyang Zhang
title	Genetic analysis and QTL mapping of seed coat color in sesame (Sesamum indicum L.).
title_short	Genetic analysis and QTL mapping of seed coat color in sesame (Sesamum indicum L.).
title_full	Genetic analysis and QTL mapping of seed coat color in sesame (Sesamum indicum L.).
title_fullStr	Genetic analysis and QTL mapping of seed coat color in sesame (Sesamum indicum L.).
title_full_unstemmed	Genetic analysis and QTL mapping of seed coat color in sesame (Sesamum indicum L.).
title_sort	genetic analysis and qtl mapping of seed coat color in sesame (sesamum indicum l.).
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/d33d086a6dbe4a04ab194b6d41bf2b96
work_keys_str_mv	AT haiyangzhang geneticanalysisandqtlmappingofseedcoatcolorinsesamesesamumindicuml AT hongmeimiao geneticanalysisandqtlmappingofseedcoatcolorinsesamesesamumindicuml AT libinwei geneticanalysisandqtlmappingofseedcoatcolorinsesamesesamumindicuml AT chunli geneticanalysisandqtlmappingofseedcoatcolorinsesamesesamumindicuml AT ruihongzhao geneticanalysisandqtlmappingofseedcoatcolorinsesamesesamumindicuml AT cuiyingwang geneticanalysisandqtlmappingofseedcoatcolorinsesamesesamumindicuml
_version_	1718423065153503232

Genetic analysis and QTL mapping of seed coat color in sesame (Sesamum indicum L.).

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