Comparison of different protocols for demineralization of cortical bone

Abstract Bone is a biological composite material consisting of two main components: collagen and mineral. Collagen is the most abundant protein in vertebrates, which makes it of high clinical and scientific interest. In this paper, we compare the composition and structure of cortical bone deminerali...

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Autores principales:	Siyuan Pang, Frances Y. Su, Amesha Green, Justin Salim, Joanna McKittrick, Iwona Jasiuk
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d33f78803bd649c29f81f33bd63accfb
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spelling	oai:doaj.org-article:d33f78803bd649c29f81f33bd63accfb2021-12-02T13:26:24ZComparison of different protocols for demineralization of cortical bone10.1038/s41598-021-86257-42045-2322https://doaj.org/article/d33f78803bd649c29f81f33bd63accfb2021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-86257-4https://doaj.org/toc/2045-2322Abstract Bone is a biological composite material consisting of two main components: collagen and mineral. Collagen is the most abundant protein in vertebrates, which makes it of high clinical and scientific interest. In this paper, we compare the composition and structure of cortical bone demineralized using several protocols: ethylene-diamine-tetraacetic acid (EDTA), formic acid (CH2O2), hydrochloric acid (HCl), and HCl/EDTA mixture. The efficiencies of these four agents were investigated by assessing the remaining mineral quantities and collagen integrity with various experimental techniques. Raman spectroscopy results show that the bone demineralized by the CH2O2 agent has highest collagen quality parameter. The HCl/EDTA mixture removes the most mineral, but it affects the collagen secondary structure as amide II bands are shifted as observed by Fourier transform infrared spectroscopy. Thermogravimetric analysis reveals that HCl and EDTA are most effective in removing the mineral with bulk measurements. In summary, we conclude that HCl best demineralizes bone, leaving the well-preserved collagen structure in the shortest time. These findings guide on the best demineralization protocol to obtain high-quality collagen from bone for clinical and scientific applications.Siyuan PangFrances Y. SuAmesha GreenJustin SalimJoanna McKittrickIwona JasiukNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Siyuan Pang Frances Y. Su Amesha Green Justin Salim Joanna McKittrick Iwona Jasiuk Comparison of different protocols for demineralization of cortical bone
description	Abstract Bone is a biological composite material consisting of two main components: collagen and mineral. Collagen is the most abundant protein in vertebrates, which makes it of high clinical and scientific interest. In this paper, we compare the composition and structure of cortical bone demineralized using several protocols: ethylene-diamine-tetraacetic acid (EDTA), formic acid (CH2O2), hydrochloric acid (HCl), and HCl/EDTA mixture. The efficiencies of these four agents were investigated by assessing the remaining mineral quantities and collagen integrity with various experimental techniques. Raman spectroscopy results show that the bone demineralized by the CH2O2 agent has highest collagen quality parameter. The HCl/EDTA mixture removes the most mineral, but it affects the collagen secondary structure as amide II bands are shifted as observed by Fourier transform infrared spectroscopy. Thermogravimetric analysis reveals that HCl and EDTA are most effective in removing the mineral with bulk measurements. In summary, we conclude that HCl best demineralizes bone, leaving the well-preserved collagen structure in the shortest time. These findings guide on the best demineralization protocol to obtain high-quality collagen from bone for clinical and scientific applications.
format	article
author	Siyuan Pang Frances Y. Su Amesha Green Justin Salim Joanna McKittrick Iwona Jasiuk
author_facet	Siyuan Pang Frances Y. Su Amesha Green Justin Salim Joanna McKittrick Iwona Jasiuk
author_sort	Siyuan Pang
title	Comparison of different protocols for demineralization of cortical bone
title_short	Comparison of different protocols for demineralization of cortical bone
title_full	Comparison of different protocols for demineralization of cortical bone
title_fullStr	Comparison of different protocols for demineralization of cortical bone
title_full_unstemmed	Comparison of different protocols for demineralization of cortical bone
title_sort	comparison of different protocols for demineralization of cortical bone
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/d33f78803bd649c29f81f33bd63accfb
work_keys_str_mv	AT siyuanpang comparisonofdifferentprotocolsfordemineralizationofcorticalbone AT francesysu comparisonofdifferentprotocolsfordemineralizationofcorticalbone AT ameshagreen comparisonofdifferentprotocolsfordemineralizationofcorticalbone AT justinsalim comparisonofdifferentprotocolsfordemineralizationofcorticalbone AT joannamckittrick comparisonofdifferentprotocolsfordemineralizationofcorticalbone AT iwonajasiuk comparisonofdifferentprotocolsfordemineralizationofcorticalbone
_version_	1718393060927209472

Comparison of different protocols for demineralization of cortical bone

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