MicroRNA-activated hydrogel scaffold generated by 3D printing accelerates bone regeneration

Bone defects remain a major threat to human health and bone tissue regeneration has become a prominent clinical demand worldwide. The combination of microRNA (miRNA) therapy with 3D printed scaffolds has always posed a challenge. It can mimic physiological bone healing processes, in which a biodegra...

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Autores principales:	Ting Pan, Wenjing Song, Hongbao Xin, Haiyue Yu, He Wang, Dandan Ma, Xiaodong Cao, Yingjun Wang
Formato:	article
Lenguaje:	EN
Publicado:	KeAi Communications Co., Ltd. 2022
Materias:	Bone repair microRNA therapy Scaffold Osteogenesis Materials of engineering and construction. Mechanics of materials TA401-492 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/d340fa5162124e2c9999154db302cd63
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spelling	oai:doaj.org-article:d340fa5162124e2c9999154db302cd632021-11-26T04:37:02ZMicroRNA-activated hydrogel scaffold generated by 3D printing accelerates bone regeneration2452-199X10.1016/j.bioactmat.2021.08.034https://doaj.org/article/d340fa5162124e2c9999154db302cd632022-04-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2452199X21004102https://doaj.org/toc/2452-199XBone defects remain a major threat to human health and bone tissue regeneration has become a prominent clinical demand worldwide. The combination of microRNA (miRNA) therapy with 3D printed scaffolds has always posed a challenge. It can mimic physiological bone healing processes, in which a biodegradable scaffold is gradually replaced by neo-tissue, and the sustained release of miRNA plays a vital role in creating an optimal osteogenic microenvironment, thus achieving promising bone repair outcomes. However, the balance between two key factors - scaffold degradation behavior and miRNA release profile - on osteogenesis and bone formation is still poorly understood. Herein, we construct a series of miRNA-activated hydrogel scaffolds (MAHSs) generated by 3D printing with different crosslinking degree to screened the interplay between scaffold degradation and miRNA release in the osteoinduction activity both in vitro and in vivo. Although MAHSs with a lower crosslinking degree (MAHS-0 and MAHS-0.25) released a higher amount of miR-29b in a sustained release profile, they degraded too fast to provide prolonged support for cell and tissue ingrowth. On the contrary, although the slow degradation of MAHSs with a higher crosslinking degree (MAHS-1 and MAHS-2.5) led to insufficient release of miR-29b, their adaptable degradation rate endowed them with more efficient osteoinductive behavior over the long term. MAHS-1 gave the most well-matched degradation rate and miR-29b release characteristics and was identified as the preferred MAHSs for accelerated bone regeneration. This study suggests that the bio-adaptable balance between scaffold degradation behavior and bioactive factors release profile plays a critical role in bone regeneration. These findings will provide a valuable reference about designing miRNAs as well as other bioactive molecules activated scaffold for tissue regeneration.Ting PanWenjing SongHongbao XinHaiyue YuHe WangDandan MaXiaodong CaoYingjun WangKeAi Communications Co., Ltd.articleBone repairmicroRNA therapyScaffoldOsteogenesisMaterials of engineering and construction. Mechanics of materialsTA401-492Biology (General)QH301-705.5ENBioactive Materials, Vol 10, Iss , Pp 1-14 (2022)
institution	DOAJ
collection	DOAJ
language	EN
topic	Bone repair microRNA therapy Scaffold Osteogenesis Materials of engineering and construction. Mechanics of materials TA401-492 Biology (General) QH301-705.5
spellingShingle	Bone repair microRNA therapy Scaffold Osteogenesis Materials of engineering and construction. Mechanics of materials TA401-492 Biology (General) QH301-705.5 Ting Pan Wenjing Song Hongbao Xin Haiyue Yu He Wang Dandan Ma Xiaodong Cao Yingjun Wang MicroRNA-activated hydrogel scaffold generated by 3D printing accelerates bone regeneration
description	Bone defects remain a major threat to human health and bone tissue regeneration has become a prominent clinical demand worldwide. The combination of microRNA (miRNA) therapy with 3D printed scaffolds has always posed a challenge. It can mimic physiological bone healing processes, in which a biodegradable scaffold is gradually replaced by neo-tissue, and the sustained release of miRNA plays a vital role in creating an optimal osteogenic microenvironment, thus achieving promising bone repair outcomes. However, the balance between two key factors - scaffold degradation behavior and miRNA release profile - on osteogenesis and bone formation is still poorly understood. Herein, we construct a series of miRNA-activated hydrogel scaffolds (MAHSs) generated by 3D printing with different crosslinking degree to screened the interplay between scaffold degradation and miRNA release in the osteoinduction activity both in vitro and in vivo. Although MAHSs with a lower crosslinking degree (MAHS-0 and MAHS-0.25) released a higher amount of miR-29b in a sustained release profile, they degraded too fast to provide prolonged support for cell and tissue ingrowth. On the contrary, although the slow degradation of MAHSs with a higher crosslinking degree (MAHS-1 and MAHS-2.5) led to insufficient release of miR-29b, their adaptable degradation rate endowed them with more efficient osteoinductive behavior over the long term. MAHS-1 gave the most well-matched degradation rate and miR-29b release characteristics and was identified as the preferred MAHSs for accelerated bone regeneration. This study suggests that the bio-adaptable balance between scaffold degradation behavior and bioactive factors release profile plays a critical role in bone regeneration. These findings will provide a valuable reference about designing miRNAs as well as other bioactive molecules activated scaffold for tissue regeneration.
format	article
author	Ting Pan Wenjing Song Hongbao Xin Haiyue Yu He Wang Dandan Ma Xiaodong Cao Yingjun Wang
author_facet	Ting Pan Wenjing Song Hongbao Xin Haiyue Yu He Wang Dandan Ma Xiaodong Cao Yingjun Wang
author_sort	Ting Pan
title	MicroRNA-activated hydrogel scaffold generated by 3D printing accelerates bone regeneration
title_short	MicroRNA-activated hydrogel scaffold generated by 3D printing accelerates bone regeneration
title_full	MicroRNA-activated hydrogel scaffold generated by 3D printing accelerates bone regeneration
title_fullStr	MicroRNA-activated hydrogel scaffold generated by 3D printing accelerates bone regeneration
title_full_unstemmed	MicroRNA-activated hydrogel scaffold generated by 3D printing accelerates bone regeneration
title_sort	microrna-activated hydrogel scaffold generated by 3d printing accelerates bone regeneration
publisher	KeAi Communications Co., Ltd.
publishDate	2022
url	https://doaj.org/article/d340fa5162124e2c9999154db302cd63
work_keys_str_mv	AT tingpan micrornaactivatedhydrogelscaffoldgeneratedby3dprintingacceleratesboneregeneration AT wenjingsong micrornaactivatedhydrogelscaffoldgeneratedby3dprintingacceleratesboneregeneration AT hongbaoxin micrornaactivatedhydrogelscaffoldgeneratedby3dprintingacceleratesboneregeneration AT haiyueyu micrornaactivatedhydrogelscaffoldgeneratedby3dprintingacceleratesboneregeneration AT hewang micrornaactivatedhydrogelscaffoldgeneratedby3dprintingacceleratesboneregeneration AT dandanma micrornaactivatedhydrogelscaffoldgeneratedby3dprintingacceleratesboneregeneration AT xiaodongcao micrornaactivatedhydrogelscaffoldgeneratedby3dprintingacceleratesboneregeneration AT yingjunwang micrornaactivatedhydrogelscaffoldgeneratedby3dprintingacceleratesboneregeneration
_version_	1718409869661306880

MicroRNA-activated hydrogel scaffold generated by 3D printing accelerates bone regeneration

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