Effect of thermal spring water on human dendritic cell inflammatory response

Yoan Eliasse1,2, Marie-Florence Galliano,3 Daniel Redoules,4 Eric Espinosa1,21INSERM U1037, Centre de Recherche en Cancérologie de Toulouse (CRCT), Toulouse F-31037, France; 2Université De Toulouse, Université Paul Sabatier, Toulouse F-31062, France; 3Research and De...

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Autores principales:	Eliasse Y, Galliano MF, Redoules D, Espinosa E
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	dendritic cells hydrotherapy inflammation naive CD4+ T cells dexamethasone Pathology RB1-214 Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/d342fb8394d74e1b87c3519c8223ac38
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spelling	oai:doaj.org-article:d342fb8394d74e1b87c3519c8223ac382021-12-02T02:03:03ZEffect of thermal spring water on human dendritic cell inflammatory response1178-7031https://doaj.org/article/d342fb8394d74e1b87c3519c8223ac382019-07-01T00:00:00Zhttps://www.dovepress.com/effect-of-thermal-spring-water-on-human-dendritic-cell-inflammatory-re-peer-reviewed-article-JIRhttps://doaj.org/toc/1178-7031Yoan Eliasse1,2, Marie-Florence Galliano,3 Daniel Redoules,4 Eric Espinosa1,21INSERM U1037, Centre de Recherche en Cancérologie de Toulouse (CRCT), Toulouse F-31037, France; 2Université De Toulouse, Université Paul Sabatier, Toulouse F-31062, France; 3Research and Development, Pierre Fabre Dermo-Cosmétique, Toulouse, France; 4Global Medical Direction, Laboratoire Dermatologique Avène, Pierre Fabre Dermo-Cosmétique, Toulouse, FranceBackground: Hydrotherapy appears as a valuable therapeutic tool in the management of patients suffering from chronic skin inflammatory diseases. Nevertheless, the underlying immune mechanisms of these beneficial effects remain poorly understood. To better understand the biological effects of thermal spring water on the immune system, we investigated the effects of Avène thermal spring water (ASW) on dendritic cells as key cells participating in the control of the immune response.Methods: Dendritic cells (DCs) were generated from human monocytes and matured with LPS in ASW-based culture medium or in dexamethasone supplemented culture medium as an anti-inflammatory treatment. The phenotypes and abilities of these DCs to produce cytokines and induce allogeneic T cell response was next assessed.Results: We showed that ASW modulated the differentiation of monocytes into DCs and impacted the DC maturation upon LPS priming. We observed a reduction of the CD83, CD86, CD1a and HLA-DR molecule expression and a decrease of IL-12 and IL-23 production whereas IL-10 production was increased. LPS-primed DCs generated in presence of ASW exhibited a reduced capacity to induce naive CD4+, T cell proliferation and IFN-γ and IL-17 production.Conclusion: Our study showed that ASW is endowed with an immunomodulatory potential. ASW limits the DC stimulatory capacity of Th1 and Th17 cell responses by impairing their maturation, IL-12 and IL-23 production and accessory cell function.Keywords: dendritic cells, hydrotherapy, inflammation, naive CD4+, T cells, dexamethasoneEliasse YGalliano MFRedoules DEspinosa EDove Medical Pressarticledendritic cellshydrotherapyinflammationnaive CD4+ T cellsdexamethasonePathologyRB1-214Therapeutics. PharmacologyRM1-950ENJournal of Inflammation Research, Vol Volume 12, Pp 181-194 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	dendritic cells hydrotherapy inflammation naive CD4+ T cells dexamethasone Pathology RB1-214 Therapeutics. Pharmacology RM1-950
spellingShingle	dendritic cells hydrotherapy inflammation naive CD4+ T cells dexamethasone Pathology RB1-214 Therapeutics. Pharmacology RM1-950 Eliasse Y Galliano MF Redoules D Espinosa E Effect of thermal spring water on human dendritic cell inflammatory response
description	Yoan Eliasse1,2, Marie-Florence Galliano,3 Daniel Redoules,4 Eric Espinosa1,21INSERM U1037, Centre de Recherche en Cancérologie de Toulouse (CRCT), Toulouse F-31037, France; 2Université De Toulouse, Université Paul Sabatier, Toulouse F-31062, France; 3Research and Development, Pierre Fabre Dermo-Cosmétique, Toulouse, France; 4Global Medical Direction, Laboratoire Dermatologique Avène, Pierre Fabre Dermo-Cosmétique, Toulouse, FranceBackground: Hydrotherapy appears as a valuable therapeutic tool in the management of patients suffering from chronic skin inflammatory diseases. Nevertheless, the underlying immune mechanisms of these beneficial effects remain poorly understood. To better understand the biological effects of thermal spring water on the immune system, we investigated the effects of Avène thermal spring water (ASW) on dendritic cells as key cells participating in the control of the immune response.Methods: Dendritic cells (DCs) were generated from human monocytes and matured with LPS in ASW-based culture medium or in dexamethasone supplemented culture medium as an anti-inflammatory treatment. The phenotypes and abilities of these DCs to produce cytokines and induce allogeneic T cell response was next assessed.Results: We showed that ASW modulated the differentiation of monocytes into DCs and impacted the DC maturation upon LPS priming. We observed a reduction of the CD83, CD86, CD1a and HLA-DR molecule expression and a decrease of IL-12 and IL-23 production whereas IL-10 production was increased. LPS-primed DCs generated in presence of ASW exhibited a reduced capacity to induce naive CD4+, T cell proliferation and IFN-γ and IL-17 production.Conclusion: Our study showed that ASW is endowed with an immunomodulatory potential. ASW limits the DC stimulatory capacity of Th1 and Th17 cell responses by impairing their maturation, IL-12 and IL-23 production and accessory cell function.Keywords: dendritic cells, hydrotherapy, inflammation, naive CD4+, T cells, dexamethasone
format	article
author	Eliasse Y Galliano MF Redoules D Espinosa E
author_facet	Eliasse Y Galliano MF Redoules D Espinosa E
author_sort	Eliasse Y
title	Effect of thermal spring water on human dendritic cell inflammatory response
title_short	Effect of thermal spring water on human dendritic cell inflammatory response
title_full	Effect of thermal spring water on human dendritic cell inflammatory response
title_fullStr	Effect of thermal spring water on human dendritic cell inflammatory response
title_full_unstemmed	Effect of thermal spring water on human dendritic cell inflammatory response
title_sort	effect of thermal spring water on human dendritic cell inflammatory response
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/d342fb8394d74e1b87c3519c8223ac38
work_keys_str_mv	AT eliassey effectofthermalspringwateronhumandendriticcellinflammatoryresponse AT gallianomf effectofthermalspringwateronhumandendriticcellinflammatoryresponse AT redoulesd effectofthermalspringwateronhumandendriticcellinflammatoryresponse AT espinosae effectofthermalspringwateronhumandendriticcellinflammatoryresponse
_version_	1718402738239307776

Effect of thermal spring water on human dendritic cell inflammatory response

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