Microtubules as platforms for assaying actin polymerization in vivo.
The actin cytoskeleton is continuously remodeled through cycles of actin filament assembly and disassembly. Filaments are born through nucleation and shaped into supramolecular structures with various essential functions. These range from contractile and protrusive assemblies in muscle and non-muscl...
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Public Library of Science (PLoS)
2011
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oai:doaj.org-article:d34b14bc521442dea6a348502f3dc5432021-11-18T06:53:56ZMicrotubules as platforms for assaying actin polymerization in vivo.1932-620310.1371/journal.pone.0019931https://doaj.org/article/d34b14bc521442dea6a348502f3dc5432011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21603613/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203The actin cytoskeleton is continuously remodeled through cycles of actin filament assembly and disassembly. Filaments are born through nucleation and shaped into supramolecular structures with various essential functions. These range from contractile and protrusive assemblies in muscle and non-muscle cells to actin filament comets propelling vesicles or pathogens through the cytosol. Although nucleation has been extensively studied using purified proteins in vitro, dissection of the process in cells is complicated by the abundance and molecular complexity of actin filament arrays. We here describe the ectopic nucleation of actin filaments on the surface of microtubules, free of endogenous actin and interfering membrane or lipid. All major mechanisms of actin filament nucleation were recapitulated, including filament assembly induced by Arp2/3 complex, formin and Spir. This novel approach allows systematic dissection of actin nucleation in the cytosol of live cells, its genetic re-engineering as well as screening for new modifiers of the process.J Margit OelkersMarlene VinzenzMaria NemethovaSonja JacobFrank P L LaiJennifer BlockMalgorzata SzczodrakEugen KerkhoffSteffen BackertKai SchlüterTheresia E B StradalJ Victor SmallStefan A KoestlerKlemens RottnerPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 5, p e19931 (2011) |
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DOAJ |
| collection |
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| language |
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| topic |
Medicine R Science Q |
| spellingShingle |
Medicine R Science Q J Margit Oelkers Marlene Vinzenz Maria Nemethova Sonja Jacob Frank P L Lai Jennifer Block Malgorzata Szczodrak Eugen Kerkhoff Steffen Backert Kai Schlüter Theresia E B Stradal J Victor Small Stefan A Koestler Klemens Rottner Microtubules as platforms for assaying actin polymerization in vivo. |
| description |
The actin cytoskeleton is continuously remodeled through cycles of actin filament assembly and disassembly. Filaments are born through nucleation and shaped into supramolecular structures with various essential functions. These range from contractile and protrusive assemblies in muscle and non-muscle cells to actin filament comets propelling vesicles or pathogens through the cytosol. Although nucleation has been extensively studied using purified proteins in vitro, dissection of the process in cells is complicated by the abundance and molecular complexity of actin filament arrays. We here describe the ectopic nucleation of actin filaments on the surface of microtubules, free of endogenous actin and interfering membrane or lipid. All major mechanisms of actin filament nucleation were recapitulated, including filament assembly induced by Arp2/3 complex, formin and Spir. This novel approach allows systematic dissection of actin nucleation in the cytosol of live cells, its genetic re-engineering as well as screening for new modifiers of the process. |
| format |
article |
| author |
J Margit Oelkers Marlene Vinzenz Maria Nemethova Sonja Jacob Frank P L Lai Jennifer Block Malgorzata Szczodrak Eugen Kerkhoff Steffen Backert Kai Schlüter Theresia E B Stradal J Victor Small Stefan A Koestler Klemens Rottner |
| author_facet |
J Margit Oelkers Marlene Vinzenz Maria Nemethova Sonja Jacob Frank P L Lai Jennifer Block Malgorzata Szczodrak Eugen Kerkhoff Steffen Backert Kai Schlüter Theresia E B Stradal J Victor Small Stefan A Koestler Klemens Rottner |
| author_sort |
J Margit Oelkers |
| title |
Microtubules as platforms for assaying actin polymerization in vivo. |
| title_short |
Microtubules as platforms for assaying actin polymerization in vivo. |
| title_full |
Microtubules as platforms for assaying actin polymerization in vivo. |
| title_fullStr |
Microtubules as platforms for assaying actin polymerization in vivo. |
| title_full_unstemmed |
Microtubules as platforms for assaying actin polymerization in vivo. |
| title_sort |
microtubules as platforms for assaying actin polymerization in vivo. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2011 |
| url |
https://doaj.org/article/d34b14bc521442dea6a348502f3dc543 |
| work_keys_str_mv |
AT jmargitoelkers microtubulesasplatformsforassayingactinpolymerizationinvivo AT marlenevinzenz microtubulesasplatformsforassayingactinpolymerizationinvivo AT marianemethova microtubulesasplatformsforassayingactinpolymerizationinvivo AT sonjajacob microtubulesasplatformsforassayingactinpolymerizationinvivo AT frankpllai microtubulesasplatformsforassayingactinpolymerizationinvivo AT jenniferblock microtubulesasplatformsforassayingactinpolymerizationinvivo AT malgorzataszczodrak microtubulesasplatformsforassayingactinpolymerizationinvivo AT eugenkerkhoff microtubulesasplatformsforassayingactinpolymerizationinvivo AT steffenbackert microtubulesasplatformsforassayingactinpolymerizationinvivo AT kaischluter microtubulesasplatformsforassayingactinpolymerizationinvivo AT theresiaebstradal microtubulesasplatformsforassayingactinpolymerizationinvivo AT jvictorsmall microtubulesasplatformsforassayingactinpolymerizationinvivo AT stefanakoestler microtubulesasplatformsforassayingactinpolymerizationinvivo AT klemensrottner microtubulesasplatformsforassayingactinpolymerizationinvivo |
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1718424258952036352 |