Isolation and Toxin Typing of Clostridium Perfringens From Sheep, Goats, and Cattle in Fars Province, Iran

Background: Clostridium perfringens is an important anaerobic bacterium found in the intestine of some livestock. It is concerned with the etiology of some diseases including enterotoxaemia. Various diseases are caused by different types of C. perfringens. Nonetheless, there is no published research...

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Autores principales:	Masoumeh Hayati, Mehrdad Shamseddini, Yahya Tahamtan, Safar Sadeghzadeh, Mohsen Manavian, Davood Nikoo
Formato:	article
Lenguaje:	EN
Publicado:	Alborz University of Medical Sciences 2020
Materias:	clostridium perfringens pcr toxin type Diseases of the digestive system. Gastroenterology RC799-869
Acceso en línea:	https://doaj.org/article/d34bcf6706e64ae998dd7e8115dd22e3
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spelling	oai:doaj.org-article:d34bcf6706e64ae998dd7e8115dd22e32021-11-18T06:56:46ZIsolation and Toxin Typing of Clostridium Perfringens From Sheep, Goats, and Cattle in Fars Province, Iran2345-33622322-586610.34172/ijep.2020.20https://doaj.org/article/d34bcf6706e64ae998dd7e8115dd22e32020-08-01T00:00:00Zhttp://enterpathog.abzums.ac.ir/PDF/ijep-4357https://doaj.org/toc/2345-3362https://doaj.org/toc/2322-5866Background: Clostridium perfringens is an important anaerobic bacterium found in the intestine of some livestock. It is concerned with the etiology of some diseases including enterotoxaemia. Various diseases are caused by different types of C. perfringens. Nonetheless, there is no published research on molecular typing and distribution of this pathogenic microorganism in Fars province. Objectives: Accordingly, our study focused on the isolation and toxin typing of C. perfringens from sheep, cattle, and goats in different parts of Fars province by the culture and the polymerase chain reaction (PCR) method. Materials and Methods: Approximately 459 fecal samples were collected and cultured on defined media for the isolation of C. perfringens. The confirmed isolates were genotyped by the PCR method using specific primers. Results: C. perfringens was isolated from 30.93% of the total samples. The results of toxin typing showed a total of 76 (54%), 13 (9%), 30 (21%), and 23 (16%) isolates as types A, B, C, and D, respectively. Conclusion: Our results indicated that C. perfringens type A was the most common type in sheep, cattle, and goats while the lowest number of isolates belonged to type B. Finally, the isolation of C. perfringens and toxin typing increase our knowledge of the epidemiology of these diseases and can help in the vaccine industry and better controlling related diseases.Masoumeh HayatiMehrdad ShamseddiniYahya TahamtanSafar SadeghzadehMohsen ManavianDavood NikooAlborz University of Medical Sciencesarticleclostridium perfringens pcr toxin type Diseases of the digestive system. GastroenterologyRC799-869ENInternational Journal of Enteric Pathogens, Vol 8, Iss 3, Pp 89-93 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	clostridium perfringens pcr toxin type Diseases of the digestive system. Gastroenterology RC799-869
spellingShingle	clostridium perfringens pcr toxin type Diseases of the digestive system. Gastroenterology RC799-869 Masoumeh Hayati Mehrdad Shamseddini Yahya Tahamtan Safar Sadeghzadeh Mohsen Manavian Davood Nikoo Isolation and Toxin Typing of Clostridium Perfringens From Sheep, Goats, and Cattle in Fars Province, Iran
description	Background: Clostridium perfringens is an important anaerobic bacterium found in the intestine of some livestock. It is concerned with the etiology of some diseases including enterotoxaemia. Various diseases are caused by different types of C. perfringens. Nonetheless, there is no published research on molecular typing and distribution of this pathogenic microorganism in Fars province. Objectives: Accordingly, our study focused on the isolation and toxin typing of C. perfringens from sheep, cattle, and goats in different parts of Fars province by the culture and the polymerase chain reaction (PCR) method. Materials and Methods: Approximately 459 fecal samples were collected and cultured on defined media for the isolation of C. perfringens. The confirmed isolates were genotyped by the PCR method using specific primers. Results: C. perfringens was isolated from 30.93% of the total samples. The results of toxin typing showed a total of 76 (54%), 13 (9%), 30 (21%), and 23 (16%) isolates as types A, B, C, and D, respectively. Conclusion: Our results indicated that C. perfringens type A was the most common type in sheep, cattle, and goats while the lowest number of isolates belonged to type B. Finally, the isolation of C. perfringens and toxin typing increase our knowledge of the epidemiology of these diseases and can help in the vaccine industry and better controlling related diseases.
format	article
author	Masoumeh Hayati Mehrdad Shamseddini Yahya Tahamtan Safar Sadeghzadeh Mohsen Manavian Davood Nikoo
author_facet	Masoumeh Hayati Mehrdad Shamseddini Yahya Tahamtan Safar Sadeghzadeh Mohsen Manavian Davood Nikoo
author_sort	Masoumeh Hayati
title	Isolation and Toxin Typing of Clostridium Perfringens From Sheep, Goats, and Cattle in Fars Province, Iran
title_short	Isolation and Toxin Typing of Clostridium Perfringens From Sheep, Goats, and Cattle in Fars Province, Iran
title_full	Isolation and Toxin Typing of Clostridium Perfringens From Sheep, Goats, and Cattle in Fars Province, Iran
title_fullStr	Isolation and Toxin Typing of Clostridium Perfringens From Sheep, Goats, and Cattle in Fars Province, Iran
title_full_unstemmed	Isolation and Toxin Typing of Clostridium Perfringens From Sheep, Goats, and Cattle in Fars Province, Iran
title_sort	isolation and toxin typing of clostridium perfringens from sheep, goats, and cattle in fars province, iran
publisher	Alborz University of Medical Sciences
publishDate	2020
url	https://doaj.org/article/d34bcf6706e64ae998dd7e8115dd22e3
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Isolation and Toxin Typing of Clostridium Perfringens From Sheep, Goats, and Cattle in Fars Province, Iran

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