Natural circularly permuted group II introns in bacteria produce RNA circles

Summary: Group II self-splicing introns are large structured RNAs that remove themselves from transcripts while simultaneously sealing the resulting gaps. Some representatives can subsequently reverse splice into DNA, accounting for their pervasive distribution in bacteria. The catalytically active...

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Autores principales:	Adam Roth, Zasha Weinberg, Koen Vanderschuren, Mitchell H. Murdock, Ronald R. Breaker
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Biological sciences Molecular biology Structural biology Bioinformatics Science Q
Acceso en línea:	https://doaj.org/article/d3591d9044474834b69ab4d1ca953928
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spelling	oai:doaj.org-article:d3591d9044474834b69ab4d1ca9539282021-11-26T04:37:57ZNatural circularly permuted group II introns in bacteria produce RNA circles2589-004210.1016/j.isci.2021.103431https://doaj.org/article/d3591d9044474834b69ab4d1ca9539282021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2589004221014024https://doaj.org/toc/2589-0042Summary: Group II self-splicing introns are large structured RNAs that remove themselves from transcripts while simultaneously sealing the resulting gaps. Some representatives can subsequently reverse splice into DNA, accounting for their pervasive distribution in bacteria. The catalytically active tertiary structure of each group II intron is assembled from six domains that are arranged in a conserved order. Here, we report structural isomers of group II introns, called CP group II ribozymes, wherein the characteristic order of domains has been altered. Domains five and six, which normally reside at the 3′ end of group II introns, instead occupy the 5′ end to form circularly permuted variants. These unusual group II intron derivatives are catalytically active and generate large linear branched and small circular RNAs, reaction products that are markedly different from those generated by canonical group II introns. The biological role of CP group II ribozymes is currently unknown.Adam RothZasha WeinbergKoen VanderschurenMitchell H. MurdockRonald R. BreakerElsevierarticleBiological sciencesMolecular biologyStructural biologyBioinformaticsScienceQENiScience, Vol 24, Iss 12, Pp 103431- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biological sciences Molecular biology Structural biology Bioinformatics Science Q
spellingShingle	Biological sciences Molecular biology Structural biology Bioinformatics Science Q Adam Roth Zasha Weinberg Koen Vanderschuren Mitchell H. Murdock Ronald R. Breaker Natural circularly permuted group II introns in bacteria produce RNA circles
description	Summary: Group II self-splicing introns are large structured RNAs that remove themselves from transcripts while simultaneously sealing the resulting gaps. Some representatives can subsequently reverse splice into DNA, accounting for their pervasive distribution in bacteria. The catalytically active tertiary structure of each group II intron is assembled from six domains that are arranged in a conserved order. Here, we report structural isomers of group II introns, called CP group II ribozymes, wherein the characteristic order of domains has been altered. Domains five and six, which normally reside at the 3′ end of group II introns, instead occupy the 5′ end to form circularly permuted variants. These unusual group II intron derivatives are catalytically active and generate large linear branched and small circular RNAs, reaction products that are markedly different from those generated by canonical group II introns. The biological role of CP group II ribozymes is currently unknown.
format	article
author	Adam Roth Zasha Weinberg Koen Vanderschuren Mitchell H. Murdock Ronald R. Breaker
author_facet	Adam Roth Zasha Weinberg Koen Vanderschuren Mitchell H. Murdock Ronald R. Breaker
author_sort	Adam Roth
title	Natural circularly permuted group II introns in bacteria produce RNA circles
title_short	Natural circularly permuted group II introns in bacteria produce RNA circles
title_full	Natural circularly permuted group II introns in bacteria produce RNA circles
title_fullStr	Natural circularly permuted group II introns in bacteria produce RNA circles
title_full_unstemmed	Natural circularly permuted group II introns in bacteria produce RNA circles
title_sort	natural circularly permuted group ii introns in bacteria produce rna circles
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/d3591d9044474834b69ab4d1ca953928
work_keys_str_mv	AT adamroth naturalcircularlypermutedgroupiiintronsinbacteriaproducernacircles AT zashaweinberg naturalcircularlypermutedgroupiiintronsinbacteriaproducernacircles AT koenvanderschuren naturalcircularlypermutedgroupiiintronsinbacteriaproducernacircles AT mitchellhmurdock naturalcircularlypermutedgroupiiintronsinbacteriaproducernacircles AT ronaldrbreaker naturalcircularlypermutedgroupiiintronsinbacteriaproducernacircles
_version_	1718409869836419072

Natural circularly permuted group II introns in bacteria produce RNA circles

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