The use of quartz crystal microbalance with dissipation (QCM-D) for studying nanoparticle-induced platelet aggregation

Maria Jose Santos-Martinez1&ndash;3, Iwona Inkielewicz-Stepniak1,4, Carlos Medina1, Kamil Rahme5,6, Deirdre M D&#39;Arcy1, Daniel Fox3, Justin D Holmes3,5, Hongzhou Zhang3, Marek Witold Radomski3,51School of Pharmacy and Pharmaceutical Sciences, 2School of Medicine, 3Center for Resea...

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Autores principales:	Santos-Martinez MJ, Inkielewicz-Stepniak I, Medina C, Rahme K, D&#39;Arcy DM, Fox D, Holmes JD, Zhang H, Radomski MW
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2012
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/d364fe18616c4df38d919a4ceb57c66b
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spelling	oai:doaj.org-article:d364fe18616c4df38d919a4ceb57c66b2021-12-02T05:55:48ZThe use of quartz crystal microbalance with dissipation (QCM-D) for studying nanoparticle-induced platelet aggregation1176-91141178-2013https://doaj.org/article/d364fe18616c4df38d919a4ceb57c66b2012-01-01T00:00:00Zhttp://www.dovepress.com/the-use-of-quartz-crystal-microbalance-with-dissipation-qcm-d-for-stud-a9057https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Maria Jose Santos-Martinez1&ndash;3, Iwona Inkielewicz-Stepniak1,4, Carlos Medina1, Kamil Rahme5,6, Deirdre M D&#39;Arcy1, Daniel Fox3, Justin D Holmes3,5, Hongzhou Zhang3, Marek Witold Radomski3,51School of Pharmacy and Pharmaceutical Sciences, 2School of Medicine, 3Center for Research on Adaptive Nanostructures and Nanodevices, Trinity College Dublin, Dublin, Ireland; 4Department of Medicinal Chemistry, Medical University of Gdansk, Gdansk, Poland; 5Materials and Supercritical Fluids Group, Department of Chemistry and the Tyndall National Institute, University College Cork, Cork, Ireland; 6Department of Sciences, Faculty of Natural and Applied Science, Notre Dame University, Zouk Mosbeh, LebanonAbstract: Interactions between blood platelets and nanoparticles have both pharmacological and toxicological significance and may lead to platelet activation and aggregation. Platelet aggregation is usually studied using light aggregometer that neither mimics the conditions found in human microvasculature nor detects microaggregates. A new method for the measurement of platelet microaggregation under flow conditions using a commercially available quartz crystal microbalance with dissipation (QCM-D) has recently been developed. The aim of the current study was to investigate if QCM-D could be used for the measurement of nanoparticle-platelet interactions. Silica, polystyrene, and gold nanoparticles were tested. The interactions were also studied using light aggregometry and flow cytometry, which measured surface abundance of platelet receptors. Platelet activation was imaged using phase contrast and scanning helium ion microscopy. QCM-D was able to measure nanoparticle-induced platelet microaggregation for all nanoparticles tested at concentrations that were undetectable by light aggregometry and flow cytometry. Microaggregates were measured by changes in frequency and dissipation, and the presence of platelets on the sensor surface was confirmed and imaged by phase contrast and scanning helium ion microscopy.Keywords: platelet aggregation, nanoparticles, light aggregometer, quartz crystal microbalance with dissipation, scanning helium ion microscopySantos-Martinez MJInkielewicz-Stepniak IMedina CRahme KD&#39;Arcy DMFox DHolmes JDZhang HRadomski MWDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2012, Iss default, Pp 243-255 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Santos-Martinez MJ Inkielewicz-Stepniak I Medina C Rahme K D&#39;Arcy DM Fox D Holmes JD Zhang H Radomski MW The use of quartz crystal microbalance with dissipation (QCM-D) for studying nanoparticle-induced platelet aggregation
description	Maria Jose Santos-Martinez1&ndash;3, Iwona Inkielewicz-Stepniak1,4, Carlos Medina1, Kamil Rahme5,6, Deirdre M D&#39;Arcy1, Daniel Fox3, Justin D Holmes3,5, Hongzhou Zhang3, Marek Witold Radomski3,51School of Pharmacy and Pharmaceutical Sciences, 2School of Medicine, 3Center for Research on Adaptive Nanostructures and Nanodevices, Trinity College Dublin, Dublin, Ireland; 4Department of Medicinal Chemistry, Medical University of Gdansk, Gdansk, Poland; 5Materials and Supercritical Fluids Group, Department of Chemistry and the Tyndall National Institute, University College Cork, Cork, Ireland; 6Department of Sciences, Faculty of Natural and Applied Science, Notre Dame University, Zouk Mosbeh, LebanonAbstract: Interactions between blood platelets and nanoparticles have both pharmacological and toxicological significance and may lead to platelet activation and aggregation. Platelet aggregation is usually studied using light aggregometer that neither mimics the conditions found in human microvasculature nor detects microaggregates. A new method for the measurement of platelet microaggregation under flow conditions using a commercially available quartz crystal microbalance with dissipation (QCM-D) has recently been developed. The aim of the current study was to investigate if QCM-D could be used for the measurement of nanoparticle-platelet interactions. Silica, polystyrene, and gold nanoparticles were tested. The interactions were also studied using light aggregometry and flow cytometry, which measured surface abundance of platelet receptors. Platelet activation was imaged using phase contrast and scanning helium ion microscopy. QCM-D was able to measure nanoparticle-induced platelet microaggregation for all nanoparticles tested at concentrations that were undetectable by light aggregometry and flow cytometry. Microaggregates were measured by changes in frequency and dissipation, and the presence of platelets on the sensor surface was confirmed and imaged by phase contrast and scanning helium ion microscopy.Keywords: platelet aggregation, nanoparticles, light aggregometer, quartz crystal microbalance with dissipation, scanning helium ion microscopy
format	article
author	Santos-Martinez MJ Inkielewicz-Stepniak I Medina C Rahme K D&#39;Arcy DM Fox D Holmes JD Zhang H Radomski MW
author_facet	Santos-Martinez MJ Inkielewicz-Stepniak I Medina C Rahme K D&#39;Arcy DM Fox D Holmes JD Zhang H Radomski MW
author_sort	Santos-Martinez MJ
title	The use of quartz crystal microbalance with dissipation (QCM-D) for studying nanoparticle-induced platelet aggregation
title_short	The use of quartz crystal microbalance with dissipation (QCM-D) for studying nanoparticle-induced platelet aggregation
title_full	The use of quartz crystal microbalance with dissipation (QCM-D) for studying nanoparticle-induced platelet aggregation
title_fullStr	The use of quartz crystal microbalance with dissipation (QCM-D) for studying nanoparticle-induced platelet aggregation
title_full_unstemmed	The use of quartz crystal microbalance with dissipation (QCM-D) for studying nanoparticle-induced platelet aggregation
title_sort	use of quartz crystal microbalance with dissipation (qcm-d) for studying nanoparticle-induced platelet aggregation
publisher	Dove Medical Press
publishDate	2012
url	https://doaj.org/article/d364fe18616c4df38d919a4ceb57c66b
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The use of quartz crystal microbalance with dissipation (QCM-D) for studying nanoparticle-induced platelet aggregation

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