Grhl3 promotes retention of epidermal cells under endocytic stress to maintain epidermal architecture in zebrafish.

Epithelia such as epidermis cover large surfaces and are crucial for survival. Maintenance of tissue homeostasis by balancing cell proliferation, cell size, and cell extrusion ensures epidermal integrity. Although the mechanisms of cell extrusion are better understood, how epithelial cells that roun...

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Autores principales:	Mandar Phatak, Shruti Kulkarni, Lee B Miles, Nazma Anjum, Sebastian Dworkin, Mahendra Sonawane
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Genetics QH426-470
Acceso en línea:	https://doaj.org/article/d365d2b7f34f4820b4ee432036ecec68
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spelling	oai:doaj.org-article:d365d2b7f34f4820b4ee432036ecec682021-12-02T20:03:18ZGrhl3 promotes retention of epidermal cells under endocytic stress to maintain epidermal architecture in zebrafish.1553-73901553-740410.1371/journal.pgen.1009823https://doaj.org/article/d365d2b7f34f4820b4ee432036ecec682021-09-01T00:00:00Zhttps://doi.org/10.1371/journal.pgen.1009823https://doaj.org/toc/1553-7390https://doaj.org/toc/1553-7404Epithelia such as epidermis cover large surfaces and are crucial for survival. Maintenance of tissue homeostasis by balancing cell proliferation, cell size, and cell extrusion ensures epidermal integrity. Although the mechanisms of cell extrusion are better understood, how epithelial cells that round up under developmental or perturbed genetic conditions are reintegrated in the epithelium to maintain homeostasis remains unclear. Here, we performed live imaging in zebrafish embryos to show that epidermal cells that round up due to membrane homeostasis defects in the absence of goosepimples/myosinVb (myoVb) function, are reintegrated into the epithelium. Transcriptome analysis and genetic interaction studies suggest that the transcription factor Grainyhead-like 3 (Grhl3) induces the retention of rounded cells by regulating E-cadherin levels. Moreover, Grhl3 facilitates the survival of MyoVb deficient embryos by regulating cell adhesion, cell retention, and epidermal architecture. Our analyses have unraveled a mechanism of retention of rounded cells and its importance in epithelial homeostasis.Mandar PhatakShruti KulkarniLee B MilesNazma AnjumSebastian DworkinMahendra SonawanePublic Library of Science (PLoS)articleGeneticsQH426-470ENPLoS Genetics, Vol 17, Iss 9, p e1009823 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Genetics QH426-470
spellingShingle	Genetics QH426-470 Mandar Phatak Shruti Kulkarni Lee B Miles Nazma Anjum Sebastian Dworkin Mahendra Sonawane Grhl3 promotes retention of epidermal cells under endocytic stress to maintain epidermal architecture in zebrafish.
description	Epithelia such as epidermis cover large surfaces and are crucial for survival. Maintenance of tissue homeostasis by balancing cell proliferation, cell size, and cell extrusion ensures epidermal integrity. Although the mechanisms of cell extrusion are better understood, how epithelial cells that round up under developmental or perturbed genetic conditions are reintegrated in the epithelium to maintain homeostasis remains unclear. Here, we performed live imaging in zebrafish embryos to show that epidermal cells that round up due to membrane homeostasis defects in the absence of goosepimples/myosinVb (myoVb) function, are reintegrated into the epithelium. Transcriptome analysis and genetic interaction studies suggest that the transcription factor Grainyhead-like 3 (Grhl3) induces the retention of rounded cells by regulating E-cadherin levels. Moreover, Grhl3 facilitates the survival of MyoVb deficient embryos by regulating cell adhesion, cell retention, and epidermal architecture. Our analyses have unraveled a mechanism of retention of rounded cells and its importance in epithelial homeostasis.
format	article
author	Mandar Phatak Shruti Kulkarni Lee B Miles Nazma Anjum Sebastian Dworkin Mahendra Sonawane
author_facet	Mandar Phatak Shruti Kulkarni Lee B Miles Nazma Anjum Sebastian Dworkin Mahendra Sonawane
author_sort	Mandar Phatak
title	Grhl3 promotes retention of epidermal cells under endocytic stress to maintain epidermal architecture in zebrafish.
title_short	Grhl3 promotes retention of epidermal cells under endocytic stress to maintain epidermal architecture in zebrafish.
title_full	Grhl3 promotes retention of epidermal cells under endocytic stress to maintain epidermal architecture in zebrafish.
title_fullStr	Grhl3 promotes retention of epidermal cells under endocytic stress to maintain epidermal architecture in zebrafish.
title_full_unstemmed	Grhl3 promotes retention of epidermal cells under endocytic stress to maintain epidermal architecture in zebrafish.
title_sort	grhl3 promotes retention of epidermal cells under endocytic stress to maintain epidermal architecture in zebrafish.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/d365d2b7f34f4820b4ee432036ecec68
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Grhl3 promotes retention of epidermal cells under endocytic stress to maintain epidermal architecture in zebrafish.

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