MMP proteolytic activity regulates cancer invasiveness by modulating integrins

Abstract Cancer invasion through dense extracellular matrices (ECMs) is mediated by matrix metalloproteinases (MMPs) which degrade the ECM thereby creating paths for migration. However, how this degradation influences the phenotype of cancer cells is not fully clear. Here we address this question by...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Alakesh Das, Melissa Monteiro, Amlan Barai, Sandeep Kumar, Shamik Sen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d3662dc961f54566b67919e8a216311e
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:d3662dc961f54566b67919e8a216311e
record_format	dspace
spelling	oai:doaj.org-article:d3662dc961f54566b67919e8a216311e2021-12-02T15:05:09ZMMP proteolytic activity regulates cancer invasiveness by modulating integrins10.1038/s41598-017-14340-w2045-2322https://doaj.org/article/d3662dc961f54566b67919e8a216311e2017-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-14340-whttps://doaj.org/toc/2045-2322Abstract Cancer invasion through dense extracellular matrices (ECMs) is mediated by matrix metalloproteinases (MMPs) which degrade the ECM thereby creating paths for migration. However, how this degradation influences the phenotype of cancer cells is not fully clear. Here we address this question by probing the function of MMPs in regulating biophysical properties of cancer cells relevant to invasion. We show that MMP catalytic activity regulates cell spreading, motility, contractility and cortical stiffness by stabilizing integrins at the membrane and activating focal adhesion kinase. Interestingly, cell rounding and cell softening on stiff gels induced by MMP inhibition is attenuated on MMP pre-conditioned surfaces. Together, our results suggest that MMP catalytic activity regulates invasiveness of cancer cells by modulating integrins.Alakesh DasMelissa MonteiroAmlan BaraiSandeep KumarShamik SenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Alakesh Das Melissa Monteiro Amlan Barai Sandeep Kumar Shamik Sen MMP proteolytic activity regulates cancer invasiveness by modulating integrins
description	Abstract Cancer invasion through dense extracellular matrices (ECMs) is mediated by matrix metalloproteinases (MMPs) which degrade the ECM thereby creating paths for migration. However, how this degradation influences the phenotype of cancer cells is not fully clear. Here we address this question by probing the function of MMPs in regulating biophysical properties of cancer cells relevant to invasion. We show that MMP catalytic activity regulates cell spreading, motility, contractility and cortical stiffness by stabilizing integrins at the membrane and activating focal adhesion kinase. Interestingly, cell rounding and cell softening on stiff gels induced by MMP inhibition is attenuated on MMP pre-conditioned surfaces. Together, our results suggest that MMP catalytic activity regulates invasiveness of cancer cells by modulating integrins.
format	article
author	Alakesh Das Melissa Monteiro Amlan Barai Sandeep Kumar Shamik Sen
author_facet	Alakesh Das Melissa Monteiro Amlan Barai Sandeep Kumar Shamik Sen
author_sort	Alakesh Das
title	MMP proteolytic activity regulates cancer invasiveness by modulating integrins
title_short	MMP proteolytic activity regulates cancer invasiveness by modulating integrins
title_full	MMP proteolytic activity regulates cancer invasiveness by modulating integrins
title_fullStr	MMP proteolytic activity regulates cancer invasiveness by modulating integrins
title_full_unstemmed	MMP proteolytic activity regulates cancer invasiveness by modulating integrins
title_sort	mmp proteolytic activity regulates cancer invasiveness by modulating integrins
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/d3662dc961f54566b67919e8a216311e
work_keys_str_mv	AT alakeshdas mmpproteolyticactivityregulatescancerinvasivenessbymodulatingintegrins AT melissamonteiro mmpproteolyticactivityregulatescancerinvasivenessbymodulatingintegrins AT amlanbarai mmpproteolyticactivityregulatescancerinvasivenessbymodulatingintegrins AT sandeepkumar mmpproteolyticactivityregulatescancerinvasivenessbymodulatingintegrins AT shamiksen mmpproteolyticactivityregulatescancerinvasivenessbymodulatingintegrins
_version_	1718388924285452288

MMP proteolytic activity regulates cancer invasiveness by modulating integrins

Ejemplares similares