Comparative transcriptome profiling of dairy goat microRNAs from dry period and peak lactation mammary gland tissues.

<h4>Background</h4>MicroRNAs (miRNAs) are small noncoding RNA molecules that serve as important post-transcriptional gene expression regulators by targeting messenger RNAs for post-transcriptional endonucleolytic cleavage or translational inhibition. miRNAs play important roles in many b...

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Autores principales:	Zhuanjian Li, Xianyong Lan, Wenjiao Guo, Jiajie Sun, Yongzhen Huang, Jing Wang, Tinghua Huang, Chuozhao Lei, Xingtang Fang, Hong Chen
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d367d7d243ed405c9d9cdb5f9ad50643
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spelling	oai:doaj.org-article:d367d7d243ed405c9d9cdb5f9ad506432021-11-18T08:03:43ZComparative transcriptome profiling of dairy goat microRNAs from dry period and peak lactation mammary gland tissues.1932-620310.1371/journal.pone.0052388https://doaj.org/article/d367d7d243ed405c9d9cdb5f9ad506432012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23300659/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>MicroRNAs (miRNAs) are small noncoding RNA molecules that serve as important post-transcriptional gene expression regulators by targeting messenger RNAs for post-transcriptional endonucleolytic cleavage or translational inhibition. miRNAs play important roles in many biological processes. Extensive high-throughput sequencing studies of miRNAs have been performed in several animal models. However, little is known about the diversity of these regulatory RNAs in goat (Capra hircus), which is one of the most important agricultural animals and the oldest domesticated species raised worldwide. Goats have long been used for their milk, meat, hair (including cashmere), and skins throughout much of the world.<h4>Results</h4>In this study, two small RNA libraries were constructed based on dry period and peak lactation dairy goat mammary gland tissues and sequenced using the Illumina-Solexa high-throughput sequencing technology. A total of 346 conserved and 95 novel miRNAs were identified in the dairy goat. miRNAs expression was confirmed by qRT-PCR in nine tissues and in the mammary gland during different stages of lactation. In addition, several candidate miRNAs that may be involved in mammary gland development and lactation were found by comparing the miRNA expression profiles in different tissues and developmental stages of the mammary gland.<h4>Conclusions</h4>This study reveals the first miRNAs profile related to the biology of the mammary gland in the dairy goat. The characterization of these miRNAs could contribute to a better understanding of the molecular mechanisms of lactation physiology and mammary gland development in the dairy goat.Zhuanjian LiXianyong LanWenjiao GuoJiajie SunYongzhen HuangJing WangTinghua HuangChuozhao LeiXingtang FangHong ChenPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 12, p e52388 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Zhuanjian Li Xianyong Lan Wenjiao Guo Jiajie Sun Yongzhen Huang Jing Wang Tinghua Huang Chuozhao Lei Xingtang Fang Hong Chen Comparative transcriptome profiling of dairy goat microRNAs from dry period and peak lactation mammary gland tissues.
description	<h4>Background</h4>MicroRNAs (miRNAs) are small noncoding RNA molecules that serve as important post-transcriptional gene expression regulators by targeting messenger RNAs for post-transcriptional endonucleolytic cleavage or translational inhibition. miRNAs play important roles in many biological processes. Extensive high-throughput sequencing studies of miRNAs have been performed in several animal models. However, little is known about the diversity of these regulatory RNAs in goat (Capra hircus), which is one of the most important agricultural animals and the oldest domesticated species raised worldwide. Goats have long been used for their milk, meat, hair (including cashmere), and skins throughout much of the world.<h4>Results</h4>In this study, two small RNA libraries were constructed based on dry period and peak lactation dairy goat mammary gland tissues and sequenced using the Illumina-Solexa high-throughput sequencing technology. A total of 346 conserved and 95 novel miRNAs were identified in the dairy goat. miRNAs expression was confirmed by qRT-PCR in nine tissues and in the mammary gland during different stages of lactation. In addition, several candidate miRNAs that may be involved in mammary gland development and lactation were found by comparing the miRNA expression profiles in different tissues and developmental stages of the mammary gland.<h4>Conclusions</h4>This study reveals the first miRNAs profile related to the biology of the mammary gland in the dairy goat. The characterization of these miRNAs could contribute to a better understanding of the molecular mechanisms of lactation physiology and mammary gland development in the dairy goat.
format	article
author	Zhuanjian Li Xianyong Lan Wenjiao Guo Jiajie Sun Yongzhen Huang Jing Wang Tinghua Huang Chuozhao Lei Xingtang Fang Hong Chen
author_facet	Zhuanjian Li Xianyong Lan Wenjiao Guo Jiajie Sun Yongzhen Huang Jing Wang Tinghua Huang Chuozhao Lei Xingtang Fang Hong Chen
author_sort	Zhuanjian Li
title	Comparative transcriptome profiling of dairy goat microRNAs from dry period and peak lactation mammary gland tissues.
title_short	Comparative transcriptome profiling of dairy goat microRNAs from dry period and peak lactation mammary gland tissues.
title_full	Comparative transcriptome profiling of dairy goat microRNAs from dry period and peak lactation mammary gland tissues.
title_fullStr	Comparative transcriptome profiling of dairy goat microRNAs from dry period and peak lactation mammary gland tissues.
title_full_unstemmed	Comparative transcriptome profiling of dairy goat microRNAs from dry period and peak lactation mammary gland tissues.
title_sort	comparative transcriptome profiling of dairy goat micrornas from dry period and peak lactation mammary gland tissues.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/d367d7d243ed405c9d9cdb5f9ad50643
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_version_	1718422450055675904

Comparative transcriptome profiling of dairy goat microRNAs from dry period and peak lactation mammary gland tissues.

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