Protein nanocoatings on synthetic polymeric nanofibrous membranes designed as carriers for skin cells

Marketa Bacakova,1,2 Julia Pajorova,1,2 Denisa Stranska,3 Daniel Hadraba,1,4 Frantisek Lopot,4 Tomas Riedel,5 Eduard Brynda,5 Margit Zaloudkova,6 Lucie Bacakova1 1Department of Biomaterials and Tissue Engineering, Institute of Physiology, Czech Academy of Sciences, 2Second Faculty of Medicine,&...

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Autores principales:	Bacakova M, Pajorova J, Stranska D, Hadraba D, Lopot F, Riedel T, Brynda E, Zaloudkova M, Bacakova L
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	skin tissue engineering nanocoating nanofibers skin cells fibrin collagen Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/d36f6a24e18046f9963de21a89d3de88
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spelling	oai:doaj.org-article:d36f6a24e18046f9963de21a89d3de882021-12-02T00:10:21ZProtein nanocoatings on synthetic polymeric nanofibrous membranes designed as carriers for skin cells1178-2013https://doaj.org/article/d36f6a24e18046f9963de21a89d3de882017-02-01T00:00:00Zhttps://www.dovepress.com/protein-nanocoatings-on-synthetic-polymeric-nanofibrous-membranes-desi-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Marketa Bacakova,1,2 Julia Pajorova,1,2 Denisa Stranska,3 Daniel Hadraba,1,4 Frantisek Lopot,4 Tomas Riedel,5 Eduard Brynda,5 Margit Zaloudkova,6 Lucie Bacakova1 1Department of Biomaterials and Tissue Engineering, Institute of Physiology, Czech Academy of Sciences, 2Second Faculty of Medicine, Charles University, Prague, 3InStar Technologies, Liberec, 4Department of Anatomy and Biomechanics, Faculty of Physical Education and Sport, Charles University, 5Department of Chemistry and Physics of Surfaces and Biointerfaces, Institute of Macromolecular Chemistry, 6Department of Composites and Carbon Materials, Institute of Rock Structure and Mechanics, Czech Academy of Sciences, Prague, Czech Republic Abstract: Protein-coated resorbable synthetic polymeric nanofibrous membranes are promising for the fabrication of advanced skin substitutes. We fabricated electrospun polylactic acid and poly(lactide-co-glycolic acid) nanofibrous membranes and coated them with fibrin or collagen I. Fibronectin was attached to a fibrin or collagen nanocoating, in order further to enhance the cell adhesion and spreading. Fibrin regularly formed a coating around individual nanofibers in the membranes, and also formed a thin noncontinuous nanofibrous mesh on top of the membranes. Collagen also coated most of the fibers of the membrane and randomly created a soft gel on the membrane surface. Fibronectin predominantly adsorbed onto a thin fibrin mesh or a collagen gel, and formed a thin nanofibrous structure. Fibrin nanocoating greatly improved the attachment, spreading, and proliferation of human dermal fibroblasts, whereas collagen nanocoating had a positive influence on the behavior of human HaCaT keratinocytes. In addition, fibrin stimulated the fibroblasts to synthesize fibronectin and to deposit it as an extracellular matrix. Fibrin coating also showed a tendency to improve the ultimate tensile strength of the nanofibrous membranes. Fibronectin attached to fibrin or to a collagen coating further enhanced the adhesion, spreading, and proliferation of both cell types. Keywords: skin-tissue engineering, nanocoating, nanofibers, skin cells, fibrin, collagenBacakova MPajorova JStranska DHadraba DLopot FRiedel TBrynda EZaloudkova MBacakova LDove Medical Pressarticleskin tissue engineeringnanocoatingnanofibersskin cellsfibrincollagenMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 1143-1160 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	skin tissue engineering nanocoating nanofibers skin cells fibrin collagen Medicine (General) R5-920
spellingShingle	skin tissue engineering nanocoating nanofibers skin cells fibrin collagen Medicine (General) R5-920 Bacakova M Pajorova J Stranska D Hadraba D Lopot F Riedel T Brynda E Zaloudkova M Bacakova L Protein nanocoatings on synthetic polymeric nanofibrous membranes designed as carriers for skin cells
description	Marketa Bacakova,1,2 Julia Pajorova,1,2 Denisa Stranska,3 Daniel Hadraba,1,4 Frantisek Lopot,4 Tomas Riedel,5 Eduard Brynda,5 Margit Zaloudkova,6 Lucie Bacakova1 1Department of Biomaterials and Tissue Engineering, Institute of Physiology, Czech Academy of Sciences, 2Second Faculty of Medicine, Charles University, Prague, 3InStar Technologies, Liberec, 4Department of Anatomy and Biomechanics, Faculty of Physical Education and Sport, Charles University, 5Department of Chemistry and Physics of Surfaces and Biointerfaces, Institute of Macromolecular Chemistry, 6Department of Composites and Carbon Materials, Institute of Rock Structure and Mechanics, Czech Academy of Sciences, Prague, Czech Republic Abstract: Protein-coated resorbable synthetic polymeric nanofibrous membranes are promising for the fabrication of advanced skin substitutes. We fabricated electrospun polylactic acid and poly(lactide-co-glycolic acid) nanofibrous membranes and coated them with fibrin or collagen I. Fibronectin was attached to a fibrin or collagen nanocoating, in order further to enhance the cell adhesion and spreading. Fibrin regularly formed a coating around individual nanofibers in the membranes, and also formed a thin noncontinuous nanofibrous mesh on top of the membranes. Collagen also coated most of the fibers of the membrane and randomly created a soft gel on the membrane surface. Fibronectin predominantly adsorbed onto a thin fibrin mesh or a collagen gel, and formed a thin nanofibrous structure. Fibrin nanocoating greatly improved the attachment, spreading, and proliferation of human dermal fibroblasts, whereas collagen nanocoating had a positive influence on the behavior of human HaCaT keratinocytes. In addition, fibrin stimulated the fibroblasts to synthesize fibronectin and to deposit it as an extracellular matrix. Fibrin coating also showed a tendency to improve the ultimate tensile strength of the nanofibrous membranes. Fibronectin attached to fibrin or to a collagen coating further enhanced the adhesion, spreading, and proliferation of both cell types. Keywords: skin-tissue engineering, nanocoating, nanofibers, skin cells, fibrin, collagen
format	article
author	Bacakova M Pajorova J Stranska D Hadraba D Lopot F Riedel T Brynda E Zaloudkova M Bacakova L
author_facet	Bacakova M Pajorova J Stranska D Hadraba D Lopot F Riedel T Brynda E Zaloudkova M Bacakova L
author_sort	Bacakova M
title	Protein nanocoatings on synthetic polymeric nanofibrous membranes designed as carriers for skin cells
title_short	Protein nanocoatings on synthetic polymeric nanofibrous membranes designed as carriers for skin cells
title_full	Protein nanocoatings on synthetic polymeric nanofibrous membranes designed as carriers for skin cells
title_fullStr	Protein nanocoatings on synthetic polymeric nanofibrous membranes designed as carriers for skin cells
title_full_unstemmed	Protein nanocoatings on synthetic polymeric nanofibrous membranes designed as carriers for skin cells
title_sort	protein nanocoatings on synthetic polymeric nanofibrous membranes designed as carriers for skin cells
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/d36f6a24e18046f9963de21a89d3de88
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_version_	1718403892521205760

Protein nanocoatings on synthetic polymeric nanofibrous membranes designed as carriers for skin cells

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